Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis
ABSTRACT: Stevia rebaudiana (Asteraceae) is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To s...
- Autores:
-
Naranjo Gómez, Esther Julia
Fernández Betin, Osman Dario
Urrea Trujillo, Aura Inés
Callejas Posada, Ricardo
Atehortúa Garcés, Lucía
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/20382
- Acceso en línea:
- http://hdl.handle.net/10495/20382
- Palabra clave:
- Ácido 2,4-Diclorofenoxiacético
2,4-Dichlorophenoxyacetic Acid
Edulcorantes naturales
Natural sweeteners
Propagación - in vitro
Propagation in vitro
Embrión somático
Somatic embryos
http://aims.fao.org/aos/agrovoc/c_27607
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis |
| dc.title.translated.spa.fl_str_mv |
Efecto del genotipo sobre la regeneración in vitro de Stevia rebaudiana a través de embriogénesis somática |
| title |
Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis |
| spellingShingle |
Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis Ácido 2,4-Diclorofenoxiacético 2,4-Dichlorophenoxyacetic Acid Edulcorantes naturales Natural sweeteners Propagación - in vitro Propagation in vitro Embrión somático Somatic embryos http://aims.fao.org/aos/agrovoc/c_27607 |
| title_short |
Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis |
| title_full |
Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis |
| title_fullStr |
Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis |
| title_full_unstemmed |
Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis |
| title_sort |
Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis |
| dc.creator.fl_str_mv |
Naranjo Gómez, Esther Julia Fernández Betin, Osman Dario Urrea Trujillo, Aura Inés Callejas Posada, Ricardo Atehortúa Garcés, Lucía |
| dc.contributor.author.none.fl_str_mv |
Naranjo Gómez, Esther Julia Fernández Betin, Osman Dario Urrea Trujillo, Aura Inés Callejas Posada, Ricardo Atehortúa Garcés, Lucía |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biotecnología |
| dc.subject.decs.none.fl_str_mv |
Ácido 2,4-Diclorofenoxiacético 2,4-Dichlorophenoxyacetic Acid |
| topic |
Ácido 2,4-Diclorofenoxiacético 2,4-Dichlorophenoxyacetic Acid Edulcorantes naturales Natural sweeteners Propagación - in vitro Propagation in vitro Embrión somático Somatic embryos http://aims.fao.org/aos/agrovoc/c_27607 |
| dc.subject.lemb.none.fl_str_mv |
Edulcorantes naturales Natural sweeteners Propagación - in vitro Propagation in vitro |
| dc.subject.agrovoc.none.fl_str_mv |
Embrión somático Somatic embryos http://aims.fao.org/aos/agrovoc/c_27607 |
| description |
ABSTRACT: Stevia rebaudiana (Asteraceae) is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To standardize an efficient and effective propagation method for the different Stevia genotypes grown in Colombia, this study evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 6-(gamma, gamma-dimethylallylamino) purine (2iP) and Zeatin on the induction and development of somatic embryos. Adenine and coconut water were also evaluated as supplements in the basal culture medium Murashige and Skoog Basal Salt Mixture (MS) with glutamine. The combination of 2,4-D (18.09 μM) and 2iP (7.38 μM) produced the highest number of somatic embryos per explant, which had well-defined characteristics. The genotype showed a significant effect on the embryogenic response. In the “SRQ-93” genotype, the formation and development of somatic embryos was achieved, whereas the genotypes “Bertoni” and “Morita II” only yielded embryogenic and non-embryogenic calli, respectively. The conversion to seedlings was achieved on the regeneration medium containing gibberellic acid (GA3) (0.29 μM) and activated charcoal. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2021-06-25T13:32:17Z |
| dc.date.available.none.fl_str_mv |
2021-06-25T13:32:17Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Naranjo EJ, Fernandez Betin O, Urrea Trujillo AI, Callejas Posada R, Atehortúa Garcés L. Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis. Acta biol. Colomb. 2016;21(1):87-98. doi: http://dx.doi.org/10.15446/abc.v21n1.47382 |
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0120-548X |
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http://hdl.handle.net/10495/20382 |
| dc.identifier.doi.none.fl_str_mv |
10.15446/abc.v21n1.47382 |
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1900-1649 |
| identifier_str_mv |
Naranjo EJ, Fernandez Betin O, Urrea Trujillo AI, Callejas Posada R, Atehortúa Garcés L. Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis. Acta biol. Colomb. 2016;21(1):87-98. doi: http://dx.doi.org/10.15446/abc.v21n1.47382 0120-548X 10.15446/abc.v21n1.47382 1900-1649 |
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http://hdl.handle.net/10495/20382 |
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eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Acta biol. Colomb. |
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98 |
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1 |
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87 |
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21 |
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Acta Biológica Colombiana |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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info:eu-repo/semantics/openAccess |
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Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología |
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Bogotá, Colombia |
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Universidad de Antioquia |
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Naranjo Gómez, Esther JuliaFernández Betin, Osman DarioUrrea Trujillo, Aura InésCallejas Posada, RicardoAtehortúa Garcés, LucíaBiotecnología2021-06-25T13:32:17Z2021-06-25T13:32:17Z2016Naranjo EJ, Fernandez Betin O, Urrea Trujillo AI, Callejas Posada R, Atehortúa Garcés L. Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis. Acta biol. Colomb. 2016;21(1):87-98. doi: http://dx.doi.org/10.15446/abc.v21n1.473820120-548Xhttp://hdl.handle.net/10495/2038210.15446/abc.v21n1.473821900-1649ABSTRACT: Stevia rebaudiana (Asteraceae) is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To standardize an efficient and effective propagation method for the different Stevia genotypes grown in Colombia, this study evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 6-(gamma, gamma-dimethylallylamino) purine (2iP) and Zeatin on the induction and development of somatic embryos. Adenine and coconut water were also evaluated as supplements in the basal culture medium Murashige and Skoog Basal Salt Mixture (MS) with glutamine. The combination of 2,4-D (18.09 μM) and 2iP (7.38 μM) produced the highest number of somatic embryos per explant, which had well-defined characteristics. The genotype showed a significant effect on the embryogenic response. In the “SRQ-93” genotype, the formation and development of somatic embryos was achieved, whereas the genotypes “Bertoni” and “Morita II” only yielded embryogenic and non-embryogenic calli, respectively. The conversion to seedlings was achieved on the regeneration medium containing gibberellic acid (GA3) (0.29 μM) and activated charcoal.RESUMEN: Stevia rebaudiana (Asteraceae), es una planta de gran importancia económica debido a sus propiedades medicinales y a la presencia de compuestos endulzantes en sus hojas, los cuales pueden sustituir la sacarosa en gran variedad de productos utilizados por personas con problemas de diabetes y obesidad. Con el propósito de estandarizar un método de propagación eficiente y efectivo para diferentes genotipos de Stevia cultivados en Colombia, en la presente investigación se evaluó el efecto sobre la inducción y desarrollo de embriones somáticos de diferentes combinaciones de los reguladores de crecimiento vegetal 2,4-D, IAA, IBA, 2iP y Zeatina, además de los suplementos adenina y agua de coco en el medio de cultivo basal Murashige y Skoog (1962), adicionado con glutamina. Con la combinación 2,4-D (18.09 μM) y 2iP (7.38 μM) se obtuvo el mayor número embriones somáticos por explante con características bien definidas. El genotipo tuvo un efecto significativo en la repuesta embriogénica, en el genotipo “SRQ-93” se logró la formación y el desarrollo de embriones somáticos, mientras que en los genotipos “Bertoni” y “Morita II”, solo se obtuvo callo embriogénico y no embriogénico respectivamente. La conversión a plántulas se alcanzó en el medio de regeneración conteniendo GA3 (0.29 μM) y carbón activado.COL000108412application/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias, Departamento de BiologíaBogotá, Colombiahttp://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesisEfecto del genotipo sobre la regeneración in vitro de Stevia rebaudiana a través de embriogénesis somáticaArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionÁcido 2,4-Diclorofenoxiacético2,4-Dichlorophenoxyacetic AcidEdulcorantes naturalesNatural sweetenersPropagación - in vitroPropagation in vitroEmbrión somáticoSomatic embryoshttp://aims.fao.org/aos/agrovoc/c_27607Acta biol. Colomb.9818721Acta Biológica ColombianaPublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/f9f4b46e-00dd-45af-a1ae-4ae271eeb709/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADORIGINALNaranjoEsther_2016_EffectsRegenerationStevia.pdfNaranjoEsther_2016_EffectsRegenerationStevia.pdfArtículo de investigaciónapplication/pdf461030https://bibliotecadigital.udea.edu.co/bitstreams/2fb7aefe-33ee-47ad-9c83-eef76659d967/download115310631ef05fe349559bced8389c0aMD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051https://bibliotecadigital.udea.edu.co/bitstreams/4c65db6b-1003-44a4-8532-14cb704a2041/downloade2060682c9c70d4d30c83c51448f4eedMD52falseAnonymousREADTEXTNaranjoEsther_2016_EffectsRegenerationStevia.pdf.txtNaranjoEsther_2016_EffectsRegenerationStevia.pdf.txtExtracted texttext/plain51432https://bibliotecadigital.udea.edu.co/bitstreams/e9a54d5b-c5d7-4666-b332-87f2c6e37c0e/download22ca53cfc2561c7a4a8b63a04eedd293MD54falseAnonymousREADTHUMBNAILNaranjoEsther_2016_EffectsRegenerationStevia.pdf.jpgNaranjoEsther_2016_EffectsRegenerationStevia.pdf.jpgGenerated Thumbnailimage/jpeg16962https://bibliotecadigital.udea.edu.co/bitstreams/6bf40069-a7ee-422e-a5ff-ac6ad96c819c/downloadada05eaf1d115ae4b4e3c7a27fb9641dMD55falseAnonymousREAD10495/20382oai:bibliotecadigital.udea.edu.co:10495/203822025-03-26 22:47:32.249http://creativecommons.org/licenses/by-nc-sa/2.5/co/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |