Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia)
ABSTRACT: Canine brucellosis is a zoonotic disease caused by Brucella canis. The establishment of intracellular replicative niches of B. canis is mediated by proteins secreted by the type IV secretion system, which is encoded by the virB operon. The characterization of such genes has been conducted...
- Autores:
-
de la Cuesta Zuluaga, Juan Jacobo
Guarín Moreno, Natalia Andrea
Sánchez Jiménez, Miryan Margot
Olivera Ángel, Martha
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2013
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- spa
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/11064
- Acceso en línea:
- http://hdl.handle.net/10495/11064
- Palabra clave:
- Genes bacterianos
Genes, Bacterial
Factores de virulencia
Virulence Factors
Brucella canis
Brucelosis
Brucellosis
http://aims.fao.org/aos/agrovoc/c_34488
http://aims.fao.org/aos/agrovoc/c_1122
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by/4.0/
| id |
UDEA2_1bd7df9573859a1cfafceb420e01f7d6 |
|---|---|
| oai_identifier_str |
oai:bibliotecadigital.udea.edu.co:10495/11064 |
| network_acronym_str |
UDEA2 |
| network_name_str |
Repositorio UdeA |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia) |
| dc.title.translated.spa.fl_str_mv |
In silico analysis of virB operon genes in Brucella canis isolated from canine kennels in the metropolitan area of the Aburrá Valley (Antioquia, Colombia) |
| title |
Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia) |
| spellingShingle |
Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia) Genes bacterianos Genes, Bacterial Factores de virulencia Virulence Factors Brucella canis Brucelosis Brucellosis http://aims.fao.org/aos/agrovoc/c_34488 http://aims.fao.org/aos/agrovoc/c_1122 |
| title_short |
Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia) |
| title_full |
Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia) |
| title_fullStr |
Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia) |
| title_full_unstemmed |
Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia) |
| title_sort |
Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia) |
| dc.creator.fl_str_mv |
de la Cuesta Zuluaga, Juan Jacobo Guarín Moreno, Natalia Andrea Sánchez Jiménez, Miryan Margot Olivera Ángel, Martha |
| dc.contributor.author.none.fl_str_mv |
de la Cuesta Zuluaga, Juan Jacobo Guarín Moreno, Natalia Andrea Sánchez Jiménez, Miryan Margot Olivera Ángel, Martha |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo Vericel |
| dc.subject.decs.none.fl_str_mv |
Genes bacterianos Genes, Bacterial Factores de virulencia Virulence Factors |
| topic |
Genes bacterianos Genes, Bacterial Factores de virulencia Virulence Factors Brucella canis Brucelosis Brucellosis http://aims.fao.org/aos/agrovoc/c_34488 http://aims.fao.org/aos/agrovoc/c_1122 |
| dc.subject.agrovoc.none.fl_str_mv |
Brucella canis Brucelosis Brucellosis |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_34488 http://aims.fao.org/aos/agrovoc/c_1122 |
| description |
ABSTRACT: Canine brucellosis is a zoonotic disease caused by Brucella canis. The establishment of intracellular replicative niches of B. canis is mediated by proteins secreted by the type IV secretion system, which is encoded by the virB operon. The characterization of such genes has been conducted in other species of the genus, but few in B. canis. We report the evaluation of the nucleotide sequences of the virB genes in seven isolates obtained from kennels in the metropolitan area of Aburrá Valley in Antioquia, Colombia. Sequences were obtained from a previous study and were compared with publicly available sequences from other Brucella strains using multiple alignment analysis and phylogenetic methods. All sequences of the native isolates tested were different from one another, even those belonging to the same kennel, which may indicate the presence of multiple bacterial clones circulating in the studied area or variations in the bacteria intra host. Key words: Brucella canis, canine brucellosis, virulence factors, bacterial genes, zoonosis. |
| publishDate |
2013 |
| dc.date.issued.none.fl_str_mv |
2013 |
| dc.date.accessioned.none.fl_str_mv |
2019-05-17T20:37:01Z |
| dc.date.available.none.fl_str_mv |
2019-05-17T20:37:01Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| status_str |
publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
de la Cuesta-Zuluaga JJ, Guarín-Moreno NA, Sánchez-Jiménez MM, Olivera-Angel M. Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia). Hechos Microbiol. 2013;4(2);84-90. |
| dc.identifier.issn.none.fl_str_mv |
2145-8898 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/11064 |
| identifier_str_mv |
de la Cuesta-Zuluaga JJ, Guarín-Moreno NA, Sánchez-Jiménez MM, Olivera-Angel M. Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia). Hechos Microbiol. 2013;4(2);84-90. 2145-8898 |
| url |
http://hdl.handle.net/10495/11064 |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Hechos Microbiol. |
| dc.relation.citationendpage.spa.fl_str_mv |
90 |
| dc.relation.citationissue.spa.fl_str_mv |
2 |
| dc.relation.citationstartpage.spa.fl_str_mv |
84 |
| dc.relation.citationvolume.spa.fl_str_mv |
4 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Hechos Microbiológicos |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
| dc.rights.uri.*.fl_str_mv |
https://creativecommons.org/licenses/by/2.5/co/ |
| dc.rights.accessrights.*.fl_str_mv |
Atribución 2.5 Colombia (CC BY 2.5 CO) |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/2.5/co/ Atribución 2.5 Colombia (CC BY 2.5 CO) http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad de Antioquia, Escuela de Microbiología |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
| institution |
Universidad de Antioquia |
| bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstreams/8da0b024-2d7d-4207-aeaf-65be03b21bdb/download https://bibliotecadigital.udea.edu.co/bitstreams/f8cd95a6-f617-47af-a942-06dfbc340a39/download https://bibliotecadigital.udea.edu.co/bitstreams/2f978d30-52ea-4b5f-b7a1-9635e50d6c3b/download https://bibliotecadigital.udea.edu.co/bitstreams/97d8a698-f542-4610-9087-36098c9a89b2/download https://bibliotecadigital.udea.edu.co/bitstreams/4801d374-94cb-420d-bbaf-344541394228/download https://bibliotecadigital.udea.edu.co/bitstreams/de52ffa8-d974-4b2b-b8d1-8282fb3bbbe0/download https://bibliotecadigital.udea.edu.co/bitstreams/b6901452-619e-480b-acd3-638b5eadb2db/download |
| bitstream.checksum.fl_str_mv |
5eba5a339fbeeeb629b2787579a5db19 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e 8a4605be74aa9ea9d79846c1fba20a33 c0a3d0d3d9372eb7f3cf9bb43878018e 45f4317341f4636c44d3f09777fa42ad |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional de la Universidad de Antioquia |
| repository.mail.fl_str_mv |
aplicacionbibliotecadigitalbiblioteca@udea.edu.co |
| _version_ |
1851052439071883264 |
| spelling |
de la Cuesta Zuluaga, Juan JacoboGuarín Moreno, Natalia AndreaSánchez Jiménez, Miryan MargotOlivera Ángel, MarthaGrupo Vericel2019-05-17T20:37:01Z2019-05-17T20:37:01Z2013de la Cuesta-Zuluaga JJ, Guarín-Moreno NA, Sánchez-Jiménez MM, Olivera-Angel M. Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia). Hechos Microbiol. 2013;4(2);84-90.2145-8898http://hdl.handle.net/10495/11064ABSTRACT: Canine brucellosis is a zoonotic disease caused by Brucella canis. The establishment of intracellular replicative niches of B. canis is mediated by proteins secreted by the type IV secretion system, which is encoded by the virB operon. The characterization of such genes has been conducted in other species of the genus, but few in B. canis. We report the evaluation of the nucleotide sequences of the virB genes in seven isolates obtained from kennels in the metropolitan area of Aburrá Valley in Antioquia, Colombia. Sequences were obtained from a previous study and were compared with publicly available sequences from other Brucella strains using multiple alignment analysis and phylogenetic methods. All sequences of the native isolates tested were different from one another, even those belonging to the same kennel, which may indicate the presence of multiple bacterial clones circulating in the studied area or variations in the bacteria intra host. Key words: Brucella canis, canine brucellosis, virulence factors, bacterial genes, zoonosis.RESUMEN: La brucelosis canina es una enfermedad zoonótica causada por Brucella canis. El establecimiento del nicho replicativo de la bacteria es mediado por proteínas secretadas mediante el sistema de secreción tipo IV, el cual es codificado por el operón virB. La caracterización de dichos genes ha sido evaluada en otras especies del género, pero pocos estudios han sido llevados a cabo en B. canis. Se reporta la evaluación de las secuencias nucleotídicas de los genes virB en siete aislamientos obtenidos de criaderos en el área metropolitana del Valle de Aburrá, en Antioquia, Colombia. Las secuencias fueron obtenidas de un estudio previo y fueron comparadas con secuencias de otras especies de Brucella disponibles públicamente, mediante análisis de alineamientos múltiples y métodos filogenéticos. Todas las secuencias de los aislamientos nativos evaluados fueron diferentes entre sí, incluso aquellas pertenecientes a un mismo criadero, lo que puede indicar la presencia de múltiples clones bacterianos circulando en el medio de estudio o variaciones de la bacteria intra hospedero. Palabras claves: Brucella canis, brucelosis canina, factores de virulencia, genes bacterianos, zoonosis.COL0078089application/pdfspaUniversidad de Antioquia, Escuela de MicrobiologíaMedellín, Colombiahttps://creativecommons.org/licenses/by/4.0/https://creativecommons.org/licenses/by/2.5/co/Atribución 2.5 Colombia (CC BY 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Análisis in silico de los genes del operón virB de Brucella canis aisladas de caninos de criaderos en el área metropolitana del Valle de Aburrá (Antioquia, Colombia)In silico analysis of virB operon genes in Brucella canis isolated from canine kennels in the metropolitan area of the Aburrá Valley (Antioquia, Colombia)Artículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionGenes bacterianosGenes, BacterialFactores de virulenciaVirulence FactorsBrucella canisBrucelosisBrucellosishttp://aims.fao.org/aos/agrovoc/c_34488http://aims.fao.org/aos/agrovoc/c_1122Hechos Microbiol.902844Hechos MicrobiológicosPublicationORIGINALDelaCuestaJuan_2013_AnalisisGenesCaninos.pdfDelaCuestaJuan_2013_AnalisisGenesCaninos.pdfArtículo de investigaciónapplication/pdf292865https://bibliotecadigital.udea.edu.co/bitstreams/8da0b024-2d7d-4207-aeaf-65be03b21bdb/download5eba5a339fbeeeb629b2787579a5db19MD51trueAnonymousREADCC-LICENSElicense_urllicense_urltext/plain; charset=utf-849https://bibliotecadigital.udea.edu.co/bitstreams/f8cd95a6-f617-47af-a942-06dfbc340a39/download4afdbb8c545fd630ea7db775da747b2fMD52falseAnonymousREADlicense_textlicense_texttext/html; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstreams/2f978d30-52ea-4b5f-b7a1-9635e50d6c3b/downloadd41d8cd98f00b204e9800998ecf8427eMD53falseAnonymousREADlicense_rdflicense_rdfLicenciaapplication/rdf+xml; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstreams/97d8a698-f542-4610-9087-36098c9a89b2/downloadd41d8cd98f00b204e9800998ecf8427eMD54falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/4801d374-94cb-420d-bbaf-344541394228/download8a4605be74aa9ea9d79846c1fba20a33MD55falseAnonymousREADTEXTDelaCuestaJuan_2013_AnalisisGenesCaninos.pdf.txtDelaCuestaJuan_2013_AnalisisGenesCaninos.pdf.txtExtracted texttext/plain40167https://bibliotecadigital.udea.edu.co/bitstreams/de52ffa8-d974-4b2b-b8d1-8282fb3bbbe0/downloadc0a3d0d3d9372eb7f3cf9bb43878018eMD56falseAnonymousREADTHUMBNAILDelaCuestaJuan_2013_AnalisisGenesCaninos.pdf.jpgDelaCuestaJuan_2013_AnalisisGenesCaninos.pdf.jpgGenerated Thumbnailimage/jpeg12007https://bibliotecadigital.udea.edu.co/bitstreams/b6901452-619e-480b-acd3-638b5eadb2db/download45f4317341f4636c44d3f09777fa42adMD57falseAnonymousREAD10495/11064oai:bibliotecadigital.udea.edu.co:10495/110642025-03-26 22:22:46.1https://creativecommons.org/licenses/by/4.0/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |
