Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia

ABSTRACT: Introduction: In this study, we aimed to identify DENV-2 subtypes in Aedes aegypti pools collected between 2011 and 2017 in a rural area of Northern Cordoba, Colombia (“La Balsa”). Methods: RT-PCR was performed to analyze the capsid/pre-membrane region (C-PrM). Sequencing and phylogenetic bayesian inference using reference DENV-2 sequences were performed. Results: Twelve pools that tested positive for DENV-2 were characterized based on the C-PrM region and grouped under the Asian/ American clade. Conclusions: This study is the first to report the DENV-2 Asian-American subtype in a rural area of Cordoba region, which is associated with severe dengue and local epidemics.

• Autores: Gallego Gómez, Juan Carlos; Atencia Pineda, Maria Claudia; Hoyos López, Richard
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2019
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/45419
• Acceso en línea: https://hdl.handle.net/10495/45419
• Palabra clave: Aedes - virología; Aedes - virology; Teorema de Bayes; Bayes Theorem; Colombia; Dengue; Virus del Dengue; Dengue Virus; Mosquitos Vectores; Mosquito Vectors; Genotipo; Genotype; Filogenia; Phylogeny; Aedes aegypti; http://aims.fao.org/aos/agrovoc/c_30482; https://id.nlm.nih.gov/mesh/D000330; https://id.nlm.nih.gov/mesh/D001499; https://id.nlm.nih.gov/mesh/D003105; https://id.nlm.nih.gov/mesh/D003715; https://id.nlm.nih.gov/mesh/D003716; https://id.nlm.nih.gov/mesh/D000072138; https://id.nlm.nih.gov/mesh/D005838; https://id.nlm.nih.gov/mesh/D010802
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/