Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines

ABSTRACT: Marine sponges are considered an important source of substances with pharmacological potential. They play a key role in the intensive investigation of promising new compounds to treat cancer and other diseases. Objective: To evaluate in CHOK1 and Jurkat cell lines, the cytotoxic, genotoxic...

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Autores:
Márquez Fernández, Diana Margarita
Martínez Martínez, Alejandro
De la Ossa, Mauricio
López Ortiz, Juan Bautista
Márquez Fernández, María Elena
Tipo de recurso:
Article of investigation
Fecha de publicación:
2016
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/13119
Acceso en línea:
http://hdl.handle.net/10495/13119
Palabra clave:
Celulas Jurkat
Jurkat Cells
Esponjas marinas
CHO­K1
Efectos citotóxicos
Genotóxicos
Antiproliferativos
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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dc.title.spa.fl_str_mv Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines
dc.title.translated.spa.fl_str_mv Actividad biológica de fracciones de la esponja marina Iotrochota birotulata en células de mamíferos
title Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines
spellingShingle Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines
Celulas Jurkat
Jurkat Cells
Esponjas marinas
CHO­K1
Efectos citotóxicos
Genotóxicos
Antiproliferativos
title_short Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines
title_full Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines
title_fullStr Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines
title_full_unstemmed Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines
title_sort Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines
dc.creator.fl_str_mv Márquez Fernández, Diana Margarita
Martínez Martínez, Alejandro
De la Ossa, Mauricio
López Ortiz, Juan Bautista
Márquez Fernández, María Elena
dc.contributor.author.none.fl_str_mv Márquez Fernández, Diana Margarita
Martínez Martínez, Alejandro
De la Ossa, Mauricio
López Ortiz, Juan Bautista
Márquez Fernández, María Elena
dc.contributor.researchgroup.spa.fl_str_mv Productos Naturales Marinos
dc.subject.decs.none.fl_str_mv Celulas Jurkat
Jurkat Cells
topic Celulas Jurkat
Jurkat Cells
Esponjas marinas
CHO­K1
Efectos citotóxicos
Genotóxicos
Antiproliferativos
dc.subject.proposal.spa.fl_str_mv Esponjas marinas
CHO­K1
Efectos citotóxicos
Genotóxicos
Antiproliferativos
description ABSTRACT: Marine sponges are considered an important source of substances with pharmacological potential. They play a key role in the intensive investigation of promising new compounds to treat cancer and other diseases. Objective: To evaluate in CHOK1 and Jurkat cell lines, the cytotoxic, genotoxic and antiproliferative effects of two fractions of I. birotulata sponge from Colombian Caribbean. Methods: The cell viability (cytotoxic effect) was determined by Trypan blue exclusion and MTT assays. Genotoxicity was assessed by single cell gel electrophoresis, the antiproliferative effect was monitored with clonogenic test, sister chromatid exchange proliferative kinetic, and accumulation function. Data was analyzed with lineal regression, oneway ANOVA, and Bonferroni tests. Results: Both cytotoxic assays showed a similar dose dependent effect for the CHOK1 and Jurkat cell lines treated with both fractions (F5 y F6) of I. birotulata. They also revealed an effect on the cell membrane and mitochondrial activity of both cell lines. Fraction F5 exhibited a greater genotoxic effect on both cell lines, which is consistent with the antiproliferation results obtained by the clonogenic assay. These results are also consistent with the inhibitory effect on the cell cycle, which was evaluated with SCE, proliferative kinetic, and the accumulation function tests. Consequently, the results showed differential sensitivity to the treatment of the Jurkat cells compared to the CHOK1 cell line. Conclusions: Together, the results show a differential effect of the two assessed fractions on cell membrane integrity, mitochondrial activity, and antiproliferative effect on both mammalian cell lines.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2020-01-14T02:58:37Z
dc.date.available.none.fl_str_mv 2020-01-14T02:58:37Z
dc.type.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv De la Ossa, M., López Ortiz, J. B., Márquez Fernández, D. M., Martínez Martínez, A., & Márquez Fernández, M. E. (2016). Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines. Revista Cubana de Farmacia, 50(4), 1-16.
dc.identifier.issn.none.fl_str_mv 0034-7515
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/13119
dc.identifier.eissn.none.fl_str_mv 1561-2988
identifier_str_mv De la Ossa, M., López Ortiz, J. B., Márquez Fernández, D. M., Martínez Martínez, A., & Márquez Fernández, M. E. (2016). Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines. Revista Cubana de Farmacia, 50(4), 1-16.
0034-7515
1561-2988
url http://hdl.handle.net/10495/13119
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationendpage.spa.fl_str_mv 16
dc.relation.citationissue.spa.fl_str_mv 4
dc.relation.citationstartpage.spa.fl_str_mv 1
dc.relation.citationvolume.spa.fl_str_mv 50
dc.relation.ispartofjournal.spa.fl_str_mv Revista Cubana de Farmacia
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dc.rights.accessrights.*.fl_str_mv Atribución-NoComercial-SinDerivadas 2.5 Colombia
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eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv Editorial Ciencias Médicas
dc.publisher.place.spa.fl_str_mv La Habana, Cuba
institution Universidad de Antioquia
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spelling Márquez Fernández, Diana MargaritaMartínez Martínez, AlejandroDe la Ossa, MauricioLópez Ortiz, Juan BautistaMárquez Fernández, María ElenaProductos Naturales Marinos2020-01-14T02:58:37Z2020-01-14T02:58:37Z2016De la Ossa, M., López Ortiz, J. B., Márquez Fernández, D. M., Martínez Martínez, A., & Márquez Fernández, M. E. (2016). Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines. Revista Cubana de Farmacia, 50(4), 1-16.0034-7515http://hdl.handle.net/10495/131191561-2988ABSTRACT: Marine sponges are considered an important source of substances with pharmacological potential. They play a key role in the intensive investigation of promising new compounds to treat cancer and other diseases. Objective: To evaluate in CHOK1 and Jurkat cell lines, the cytotoxic, genotoxic and antiproliferative effects of two fractions of I. birotulata sponge from Colombian Caribbean. Methods: The cell viability (cytotoxic effect) was determined by Trypan blue exclusion and MTT assays. Genotoxicity was assessed by single cell gel electrophoresis, the antiproliferative effect was monitored with clonogenic test, sister chromatid exchange proliferative kinetic, and accumulation function. Data was analyzed with lineal regression, oneway ANOVA, and Bonferroni tests. Results: Both cytotoxic assays showed a similar dose dependent effect for the CHOK1 and Jurkat cell lines treated with both fractions (F5 y F6) of I. birotulata. They also revealed an effect on the cell membrane and mitochondrial activity of both cell lines. Fraction F5 exhibited a greater genotoxic effect on both cell lines, which is consistent with the antiproliferation results obtained by the clonogenic assay. These results are also consistent with the inhibitory effect on the cell cycle, which was evaluated with SCE, proliferative kinetic, and the accumulation function tests. Consequently, the results showed differential sensitivity to the treatment of the Jurkat cells compared to the CHOK1 cell line. Conclusions: Together, the results show a differential effect of the two assessed fractions on cell membrane integrity, mitochondrial activity, and antiproliferative effect on both mammalian cell lines.RESUMEN: Las esponjas marinas son una fuente importante de compuestos con propiedades farmacológicas potenciales. Desempeñan una función esencial en la intensa en la búsqueda de nuevas y prometedores compuestos que brinden tratamiento para el cáncer y otras enfermedades. Objetivo: Evaluar los efectos citotóxico, genotóxico y antiproliferativo en las líneas celulares CHOK1 y Jurkat de dos fracciones de la esponja Iotrochota birotulata del Caribe colombiano. Métodos: La viabilidad celular (efecto citotóxico) se determinó mediante ensayos de azul de Tripano y MTT. La genotoxicidad se evaluó mediante la electroforesis en gel de células individuales y el efecto antiproliferativo se monitoreó con pruebas clonogénica, cinética proliferativa por intercambio de cromátides hermanas y la función de acumulación. Los datos se analizaron mediante regresión lineal, ANOVA de una vía, y la prueba de Bonferroni. Resultados: Ambas pruebas citotóxicas mostraron un efecto similar dependiente de la dosis en las líneas celulares CHOK1 y Jurkat tratadas con dos fracciones (F5 y F6) de I. birotulata. Las pruebas citotóxicas también revelaron efectos en la membrana celular y en la actividad mitocondrial en ambas líneas celulares. La fracción F5 mostró mayor efecto genotóxico en las dos líneas celulares, consistente con los resultados de antiproliferación del ensayo clonogénico y con el efecto inhibitorio en el ciclo celular evaluado por las pruebas de intercambio de cromátides hermanas, cinética proliferativa y función de acumulación. Por consiguiente, los resultados revelaron mayor sensibilidad diferencial al tratamiento en las células Jurkat comparada con la línea celular CHOK1. Conclusiones: Los datos obtenidos demuestran el efecto diferencial de las fracciones F5 y F6, en la integridad de la membrana celular, la actividad mitocondrial y el efecto antiproliferativo en las dos líneas celulares de mamíferos evaluadas.COL0015043application/pdfengEditorial Ciencias MédicasLa Habana, Cubahttp://creativecommons.org/licenses/by-nc-nd/2.5/co/https://creativecommons.org/licenses/by-nc-nd/4.0/Atribución-NoComercial-SinDerivadas 2.5 Colombiainfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell linesActividad biológica de fracciones de la esponja marina Iotrochota birotulata en células de mamíferosArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionCelulas JurkatJurkat CellsEsponjas marinasCHO­K1Efectos citotóxicosGenotóxicosAntiproliferativos164150Revista Cubana de Farmaciahttp://www.revfarmacia.sld.cu/index.php/far/rt/printerFriendly/63/67PublicationCC-LICENSElicense_textlicense_texttext/html; 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