Determinación de péptidos bioactivos del chachafruto (Erythrina edulis)

En el contexto actual de crecimiento poblacional y preocupación por la seguridad alimentaria y el impacto ambiental, se ha intensificado la investigación sobre fuentes de proteínas sostenibles y nutritivas. Erythrina edulis (chachafruto) es una leguminosa endémica de América del Sur, conocida por su...

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Autores:: Correa Caleño, Jessica Leonor

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2025

Institución:: Universidad de Antioquia

Repositorio:: Repositorio UdeA

Idioma:: spa

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network_acronym_str	UDEA2
network_name_str	Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv	Determinación de péptidos bioactivos del chachafruto (Erythrina edulis)
title	Determinación de péptidos bioactivos del chachafruto (Erythrina edulis)
spellingShingle	Determinación de péptidos bioactivos del chachafruto (Erythrina edulis) Erythrina edulis Alimento funcional Functional foods Hidrolizado de proteínas Protein hydrolysates Propiedad antioxidante Antioxidant properties Factor inmunológico Immunological factors Hidrólisis enzimática Enzymatic hydrolysis Seguridad alimentaria Food security Péptidos bioactivos http://aims.fao.org/aos/agrovoc/c_2f007684 http://aims.fao.org/aos/agrovoc/c_3732 http://aims.fao.org/aos/agrovoc/c_11805097 http://aims.fao.org/aos/agrovoc/c_29621 http://aims.fao.org/aos/agrovoc/c_27512 http://aims.fao.org/aos/agrovoc/c_10967 http://id.loc.gov/authorities/subjects/sh90001362 ODS 2: Hambre cero. Poner fin al hambre, lograr la seguridad alimentaria y la mejora de la nutrición y promover la agricultura sostenible ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades
title_short	Determinación de péptidos bioactivos del chachafruto (Erythrina edulis)
title_full	Determinación de péptidos bioactivos del chachafruto (Erythrina edulis)
title_fullStr	Determinación de péptidos bioactivos del chachafruto (Erythrina edulis)
title_full_unstemmed	Determinación de péptidos bioactivos del chachafruto (Erythrina edulis)
title_sort	Determinación de péptidos bioactivos del chachafruto (Erythrina edulis)
dc.creator.fl_str_mv	Correa Caleño, Jessica Leonor
dc.contributor.advisor.none.fl_str_mv	Zapata Montoya, José Edgar
dc.contributor.author.none.fl_str_mv	Correa Caleño, Jessica Leonor
dc.contributor.researchgroup.none.fl_str_mv	Grupo de Nutrición y Tecnología de Alimentos
dc.contributor.jury.none.fl_str_mv	Hernández Ledesma, Blanca Jiménez Aliaga, Karim Lizeth Martínez Villaluenga, Cristina
dc.subject.lcsh.none.fl_str_mv	Erythrina edulis
topic	Erythrina edulis Alimento funcional Functional foods Hidrolizado de proteínas Protein hydrolysates Propiedad antioxidante Antioxidant properties Factor inmunológico Immunological factors Hidrólisis enzimática Enzymatic hydrolysis Seguridad alimentaria Food security Péptidos bioactivos http://aims.fao.org/aos/agrovoc/c_2f007684 http://aims.fao.org/aos/agrovoc/c_3732 http://aims.fao.org/aos/agrovoc/c_11805097 http://aims.fao.org/aos/agrovoc/c_29621 http://aims.fao.org/aos/agrovoc/c_27512 http://aims.fao.org/aos/agrovoc/c_10967 http://id.loc.gov/authorities/subjects/sh90001362 ODS 2: Hambre cero. Poner fin al hambre, lograr la seguridad alimentaria y la mejora de la nutrición y promover la agricultura sostenible ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades
dc.subject.agrovoc.none.fl_str_mv	Alimento funcional Functional foods Hidrolizado de proteínas Protein hydrolysates Propiedad antioxidante Antioxidant properties Factor inmunológico Immunological factors Hidrólisis enzimática Enzymatic hydrolysis Seguridad alimentaria Food security
dc.subject.proposal.spa.fl_str_mv	Péptidos bioactivos
dc.subject.agrovocuri.none.fl_str_mv	http://aims.fao.org/aos/agrovoc/c_2f007684 http://aims.fao.org/aos/agrovoc/c_3732 http://aims.fao.org/aos/agrovoc/c_11805097 http://aims.fao.org/aos/agrovoc/c_29621 http://aims.fao.org/aos/agrovoc/c_27512 http://aims.fao.org/aos/agrovoc/c_10967
dc.subject.lcshuri.none.fl_str_mv	http://id.loc.gov/authorities/subjects/sh90001362
dc.subject.ods.none.fl_str_mv	ODS 2: Hambre cero. Poner fin al hambre, lograr la seguridad alimentaria y la mejora de la nutrición y promover la agricultura sostenible ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades
description	En el contexto actual de crecimiento poblacional y preocupación por la seguridad alimentaria y el impacto ambiental, se ha intensificado la investigación sobre fuentes de proteínas sostenibles y nutritivas. Erythrina edulis (chachafruto) es una leguminosa endémica de América del Sur, conocida por su alto contenido proteico y calidad nutricional. Este estudio investigó los péptidos bioactivos derivados de E. edulis, enfocándose en su potencial antioxidante y efectos inmunomoduladores. El objetivo principal fue evaluar los péptidos obtenidos mediante hidrólisis enzimática de las proteínas de esta leguminosa. La metodología incluyó la extracción de proteínas de semillas y vainas, seguida de su hidrólisis con Alcalasa y estudios de digestión gastrointestinal simulada. Los resultados mostraron que tanto las semillas como las vainas son fuentes ricas en proteínas de alta calidad. La hidrólisis enzimática mejoró significativamente la capacidad antioxidante, especialmente en las fracciones de bajo peso molecular (<3 kDa). Los estudios de digestión gastrointestinal indican que los péptidos bioactivos liberados mantienen o mejoran su actividad antioxidante y presentan efectos inmunomoduladores. En conclusión, E. edulis es una fuente prometedora de péptidos bioactivos con aplicaciones potenciales en alimentos funcionales y nutracéuticos. La investigación destaca el valor de los subproductos de esta leguminosa, promoviendo su uso integral y contribuyendo a la sostenibilidad y reducción de residuos. Los péptidos obtenidos no solo tienen propiedades antioxidantes, sino también potenciales efectos antidiabéticos, antihipertensivos y antiinflamatorios, lo que los convierte en candidatos prometedores para el desarrollo de productos nutracéuticos y farmacéuticos.
publishDate	2025
dc.date.accessioned.none.fl_str_mv	2025-06-11T20:19:28Z
dc.date.issued.none.fl_str_mv	2025
dc.date.available.none.fl_str_mv	2027-06-11
dc.type.none.fl_str_mv	Trabajo de grado - Doctorado
dc.type.coar.none.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.redcol.none.fl_str_mv	http://purl.org/redcol/resource_type/TD
dc.type.content.none.fl_str_mv	Text
dc.type.coarversion.none.fl_str_mv	http://purl.org/coar/version/c_b1a7d7d4d402bcce
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.none.fl_str_mv	info:eu-repo/semantics/draft
format	http://purl.org/coar/resource_type/c_db06
status_str	draft
dc.identifier.citation.none.fl_str_mv	Correa Caleño, J. (2025). Determinación de péptidos bioactivos del chachafruto (Erythrina edulis). [Tesis doctoral]. Universidad de Antioquia, Medellín, Colombia.
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/10495/46332
identifier_str_mv	Correa Caleño, J. (2025). Determinación de péptidos bioactivos del chachafruto (Erythrina edulis). [Tesis doctoral]. Universidad de Antioquia, Medellín, Colombia.
url	https://hdl.handle.net/10495/46332
dc.language.iso.none.fl_str_mv	spa
language	spa
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spelling	Zapata Montoya, José EdgarCorrea Caleño, Jessica LeonorGrupo de Nutrición y Tecnología de AlimentosHernández Ledesma, BlancaJiménez Aliaga, Karim LizethMartínez Villaluenga, Cristina2025-06-11T20:19:28Z2027-06-112025Correa Caleño, J. (2025). Determinación de péptidos bioactivos del chachafruto (Erythrina edulis). [Tesis doctoral]. Universidad de Antioquia, Medellín, Colombia.https://hdl.handle.net/10495/46332En el contexto actual de crecimiento poblacional y preocupación por la seguridad alimentaria y el impacto ambiental, se ha intensificado la investigación sobre fuentes de proteínas sostenibles y nutritivas. Erythrina edulis (chachafruto) es una leguminosa endémica de América del Sur, conocida por su alto contenido proteico y calidad nutricional. Este estudio investigó los péptidos bioactivos derivados de E. edulis, enfocándose en su potencial antioxidante y efectos inmunomoduladores. El objetivo principal fue evaluar los péptidos obtenidos mediante hidrólisis enzimática de las proteínas de esta leguminosa. La metodología incluyó la extracción de proteínas de semillas y vainas, seguida de su hidrólisis con Alcalasa y estudios de digestión gastrointestinal simulada. Los resultados mostraron que tanto las semillas como las vainas son fuentes ricas en proteínas de alta calidad. La hidrólisis enzimática mejoró significativamente la capacidad antioxidante, especialmente en las fracciones de bajo peso molecular (<3 kDa). Los estudios de digestión gastrointestinal indican que los péptidos bioactivos liberados mantienen o mejoran su actividad antioxidante y presentan efectos inmunomoduladores. En conclusión, E. edulis es una fuente prometedora de péptidos bioactivos con aplicaciones potenciales en alimentos funcionales y nutracéuticos. La investigación destaca el valor de los subproductos de esta leguminosa, promoviendo su uso integral y contribuyendo a la sostenibilidad y reducción de residuos. Los péptidos obtenidos no solo tienen propiedades antioxidantes, sino también potenciales efectos antidiabéticos, antihipertensivos y antiinflamatorios, lo que los convierte en candidatos prometedores para el desarrollo de productos nutracéuticos y farmacéuticos.In the current context of population growth and concerns about food security and environmental impact, research on sustainable and nutritious protein sources has intensified. Erythrina edulis (chachafruto) is a legume endemic to South America, known for its high protein content and nutritional quality. This study investigated the bioactive peptides derived from E. edulis, focusing on their antioxidant potential and immunomodulatory effects. The main objective was to evaluate the peptides obtained through enzymatic hydrolysis of the proteins from this legume. The methodology included the extraction of proteins from seeds and pods, followed by hydrolysis with Alcalase and simulated gastrointestinal digestion studies. The results showed that both seeds and pods are rich sources of high-quality proteins. Enzymatic hydrolysis significantly enhanced the antioxidant capacity, especially in low molecular weight fractions (<3 kDa). Gastrointestinal digestion studies indicated that the released bioactive peptides maintain or improve their antioxidant activity and exhibit immunomodulatory effects. In conclusion, E. edulis is a promising source of bioactive peptides with potential applications in functional foods and nutraceuticals. The research highlights the value of the by-products of this legume, promoting their integral use and contributing to sustainability and waste reduction. The obtained peptides not only have antioxidant properties but also potential antidiabetic, antihypertensive, and anti-inflammatory effects, making them promising candidates for the development of nutraceutical and pharmaceutical products.Resumen 15 Abstract 16 1. Introducción 17 1.1. Planteamiento del problema 20 1.2. Objetivos 21 1.2.1. Objetivo general 21 1.2.2. Objetivos específicos 21 1.4 Referencias 22 2. Marco teórico 26 2.1. Generalidades de las leguminosas 26 2.1.1. Leguminosas como fuente de proteína 26 2.2. Erythrina edulis 27 2.2.1. Distribución y hábitat 27 2.2.2. Descripción 27 2.2.3. Aplicaciones y usos de la proteína y otros compuestos de E. edulis 28 2.3. Extracción de proteínas vegetales 31 2.4. Hidrólisis enzimática de proteínas 32 2.4.1. Determinación del grado de hidrólisis 33 2.4.2. Aplicaciones de los hidrolizados proteicos 34 2.5. Péptidos bioactivos 37 2.5.1. Obtención de péptidos bioactivos 38 2.5.1.1. Péptidos obtenidos por hidrólisis enzimática. 38 2.5.1.2. Péptidos obtenidos por digestión in vitro 40 2.5.2. Tipos de péptidos bioactivos 41 2.5.2.1. Péptidos con actividad opioide 41 2.5.2.2. Péptidos inmunomoduladores 41 2.5.2.3. Péptidos antioxidantes 42 2.5.3. Péptidos Bioactivos derivados de alimentos 44 2.5.4. Péptidos en leguminosas 45 2.6. Referencias 47 3. HIDRÓLISIS ENZIMÁTICA DE CONCENTRADOS PROTEÍCOS DE SEMILLAS Y VAINAS DE CHACHAFRUTO 57 3.1. Introducción 57 3.2. Materiales y métodos 59 3.2.2. Obtención del concentrado proteico de semillas y vainas (CPC - CPV) 60 3.2.3. Caracterización fisicoquímica 60 3.2.3.1. Determinación de fracciones proteicas 61 3.2.3.2. Determinación de polifenoles totales 62 3.2.3.3. Flavonoides totales 62 3.2.3.4. Cuantificación de clorofila 63 3.2.4. Cuantificación de aminoácidos 63 3.2.5. Hidrólisis enzimática 64 3.2.6. Fraccionamiento por tamaño molecular y por hidrofobicidad del Hidrolizado enzimático de semilla (HES) y de vaina (HEV) 65 3.2.7. Capacidad Antioxidante 65 3.2.7.1. Ensayo de captación de radicales ABTS•+ 66 3.2.7.2. Actividad reductora de Fe+3 (Ferric Reducing/Antioxidant Power) FRAP 66 3.2.7.3. Capacidad de absorción de radicales de oxígeno (ORAC) 66 3.2.8. Secado por atomización 67 3.2.9. Análisis estadístico 67 3.3. Resultados y discusión 68 3.3.1. Caracterización fisicoquímica 68 3.3.2. Fraccionamiento de proteínas presentes en semillas y vainas de E. edulis. 70 3.3.3. Composición aminoacídica de semillas y vainas de E. edulis. 71 3.3.4. Hidrólisis enzimática 72 3.3.5. Capacidad antioxidante 74 3.4. Conclusiones 82 3.5. Referencias 82 3.6. Anexos 88 4. DIGESTIÓN GASTROINTESTINAL DEL CONCENTRADO PROTEICO DE SEMILLAS DE CHACHAFRUTO 91 4.1. Introducción 91 4.2. Materiales y métodos 92 4.2.1. Materiales 92 4.2.2. Análisis de la Harina de Semilla 93 4.2.3. Concentrado Proteico de la semilla de Chachafruto (CPC) 93 4.2.4. Digestión gastrointestinal simulada 94 4.2.5. Actividad antioxidante por ensayos bioquímicos 94 4.2.6. Efectos protectores en macrófagos RAW264.7 95 4.2.6.1. Cultivo celular 95 4.2.6.2. Efectos en la Viabilidad Celular 96 4.2.6.3. Efectos en la Generación de EROS 96 4.2.6.4. Efectos en los Niveles de NO 96 4.2.7. Análisis estadístico 97 4.3. Resultados y discusión 97 4.3.1. Caracterización del concentrado de proteína de semilla de chachafruto (CPC): comportamiento bajo digestión gastrointestinal simulada 97 4.3.2. Impacto de la digestión gastrointestinal en la actividad antioxidante del concentrado de proteína de semilla de chachafruto (CPC) 99 4.3.3. Efecto del concentrado de proteina de chachafruto (CPC) en Macrófagos RAW264.7 bajo condiciones basales y estimuladas 101 4.4. Conclusiones 107 4.5. Referencias 107 5. DIGESTIÓN GASTROINTESTINAL DEL HIDROLIZADO DEL CONCENTRADO PROTEICO DE LA SEMILLA DE CHACHAFRUTO 111 5.1. Introducción 111 5.2. Materiales y métodos 112 5.2.1. Electroforesis (SDS-PAGE) del Hidrolizado de Proteína de Semilla de Chachafruto (HES) 112 5.2.2. Digestión Gastrointestinal Simulada 113 5.2.3. Distribución del peso molecular 114 5.2.4. Actividad antioxidante por ensayos bioquímicos 114 5.2.5. Efecto protector en células de macrófagos RAW264.7 115 5.2.5.1. Cultivo celular 115 5.2.5.2. Efectos sobre la viabilidad celular 115 5.2.5.3. Efectos sobre la generación de especies reactivas de oxígeno (EROS) 115 5.2.5.4. Efectos sobre los niveles de óxido nítrico (NO) 116 5.2.6. Análisis estadístico 116 5.3. Resultados y discusión 116 5.3.1. SDS-PAGE del Hidrolizado de Proteína de Semilla de Chachafruto (HES) 116 5.3.2. Comportamiento del Hidrolizado de Concentrado de Proteína de Chachafruto (HES) bajo Digestión Gastrointestinal Simulada 118 5.3.3. Efecto de la digestión gastrointestinal simulada en la actividad antioxidante del hidrolizado de proteína de chachafruto (HES) 119 5.3.4. Impacto de la digestión gastrointestinal simulada en los efectos moduladores del HES en un modelo de células inmunológicas 122 5.4. Conclusiones 127 5.5. Referencias 127 6. PEPTIDOS BIOACTIVOS DEL CHACHAFRUTO: UN ENFOQUE EX VIVO E IN SILICO 131 6.1. Introducción 131 6.2. Materiales y métodos 132 6.2.1. Ensayos antioxidantes ex vivo 132 6.2.1.1. Cultivo celular 133 6.2.1.2. Condiciones de tratamiento 133 6.2.1.3. Determinación de Especies Reactivas de Oxígeno (EROS) intracelulares 133 6.2.2. Identificación de las proteínas de E. edulis 134 6.2.2.1. Digestión de las proteínas en gel (Stacking gel) 134 6.2.3. Digestión gastrointestinal in silico de E. edulis 136 6.2.4. Predicción de la bioactividad in silico 136 6.2.5. Análisis estadístico 137 6.3. Resultados y discusión 137 6.3.1. Ensayos antioxidantes Ex vivo 137 6.3.1.1. Efectos del DGIHES y su fracción <3 kDa sobre la generación de EROS intracelular en células Caco-2 137 6.3.2. Identificación de las proteínas de E. edulis 139 6.3.2.1. Clasificación funcional de las proteínas identificadas 141 6.3.3. Digestión in silico del proteoma de E. edulis 145 6.3.3.1. Digestión con Alcalasa 145 6.3.3.1.1. Péptidos antioxidantes de E. edulis 147 6.3.3.1.2. Péptidos antimicrobianos de E. edulis 150 6.3.3.1.3. Péptidos antidiabéticos de E. edulis 152 6.3.3.1.4. Péptidos antihipertensivos de E. edulis 154 6.3.3.1.5. Péptidos de E. edulis con actividad antiinflamatoria 156 6.3.3.2. Digestión completa de las proteínas de E. edulis 159 6.3.3.2.1. Estudio ADMET de los péptidos multifuncionales de E. edulis 161 6.4. Conclusiones 165 6.5. Referencias 166 6.6. Anexos 173 7. CONCLUSIONES GENERALES 185 8. 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

Determinación de péptidos bioactivos del chachafruto (Erythrina edulis)

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