Extracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidante
ABSTRACT: Ethanolic extracts from P. peruviana showed cytotoxic activity against different cancer cell lines. Objective: To evaluate the antiproliferative activity of aqueous extract of the fruit golden berry in colon cancer cells. Materials and Methods: We analyzed cytotoxicity and selectivity inde...
- Autores:
-
Areiza Mazo, Natalia
Maldonado Celis, María Elena
Rojano, Benjamín
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2013
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- spa
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/11616
- Acceso en línea:
- http://hdl.handle.net/10495/11616
- Palabra clave:
- Physalis peruviana
Cáncer colorrectal
Colorectal cancer
Ciclo celular
Cellular cycle
Apoptosis
Antioxidantes
Antioxidants
Anticancerígenos
Anti-cancer
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
| id |
UDEA2_0e35be8677b7f85c102f5ffd47370b7e |
|---|---|
| oai_identifier_str |
oai:bibliotecadigital.udea.edu.co:10495/11616 |
| network_acronym_str |
UDEA2 |
| network_name_str |
Repositorio UdeA |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Extracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidante |
| dc.title.translated.spa.fl_str_mv |
Aqueous extract of golden berry (Physalis peruviana) : antiproliferative, apoptotic and antioxidant activities |
| title |
Extracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidante |
| spellingShingle |
Extracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidante Physalis peruviana Cáncer colorrectal Colorectal cancer Ciclo celular Cellular cycle Apoptosis Antioxidantes Antioxidants Anticancerígenos Anti-cancer |
| title_short |
Extracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidante |
| title_full |
Extracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidante |
| title_fullStr |
Extracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidante |
| title_full_unstemmed |
Extracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidante |
| title_sort |
Extracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidante |
| dc.creator.fl_str_mv |
Areiza Mazo, Natalia Maldonado Celis, María Elena Rojano, Benjamín |
| dc.contributor.author.none.fl_str_mv |
Areiza Mazo, Natalia Maldonado Celis, María Elena Rojano, Benjamín |
| dc.subject.none.fl_str_mv |
Physalis peruviana Cáncer colorrectal Colorectal cancer Ciclo celular Cellular cycle Apoptosis Antioxidantes Antioxidants Anticancerígenos Anti-cancer |
| topic |
Physalis peruviana Cáncer colorrectal Colorectal cancer Ciclo celular Cellular cycle Apoptosis Antioxidantes Antioxidants Anticancerígenos Anti-cancer |
| description |
ABSTRACT: Ethanolic extracts from P. peruviana showed cytotoxic activity against different cancer cell lines. Objective: To evaluate the antiproliferative activity of aqueous extract of the fruit golden berry in colon cancer cells. Materials and Methods: We analyzed cytotoxicity and selectivity index (SI) (MTT), antiproliferation (sulforhodamine-B), apoptosis (cell cycle, Annexin-V, receptors TRAIL-DR4/-DR5 and caspase-3), and antioxidant activity (content of total carotenoids and flavonoids). Results: The aqueous extract showed cytotoxic and antiproliferative effect on SW480 (IC50 = 44.2 µg/ml, SI = 11.6) and SW620 (IC50 = 85.1 µg/ml, SI = 6.0). The hypodiploid SW480 and SW620 cells increased 13% and 12% respectively as well as apoptotic SW480 and SW620 cells (19% and 21 %, respectively), expression of receptors TRAIL-DR4 /-DR5 (SW480: +59% / +53%; SW620: +67% / +65%) and activation of caspase-3. The aqueous extract scavenged OH- radicals, showed low oxygen radical capacity and low scavenging capacity for reactive oxygen and nitrogen species. The total flavonoids and carotenoids contente was 487.1 mg catechin y 0.9 mg β-carotene by 100 g powder, respectively. Conclusions: These findings suggest that golden berry may be a promising source of bioactive compounds with chemopreventive activity against human colon cancer. |
| publishDate |
2013 |
| dc.date.issued.none.fl_str_mv |
2013 |
| dc.date.accessioned.none.fl_str_mv |
2019-08-06T18:39:00Z |
| dc.date.available.none.fl_str_mv |
2019-08-06T18:39:00Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| status_str |
publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Areiza-Mazo N, Maldonado ME, Rojano B. Extracto acuoso de uchuva (Physalis peruviana): actividades antiproliferativa, apoptótica y antioxidante. Perspect. Nutr. Humana.. 2013;15: 41-55. |
| dc.identifier.issn.none.fl_str_mv |
0124-4108 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/11616 |
| dc.identifier.eissn.none.fl_str_mv |
2248-454X |
| identifier_str_mv |
Areiza-Mazo N, Maldonado ME, Rojano B. Extracto acuoso de uchuva (Physalis peruviana): actividades antiproliferativa, apoptótica y antioxidante. Perspect. Nutr. Humana.. 2013;15: 41-55. 0124-4108 2248-454X |
| url |
http://hdl.handle.net/10495/11616 |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Perspect. Nutr. Humana. |
| dc.relation.citationendpage.spa.fl_str_mv |
55 |
| dc.relation.citationissue.spa.fl_str_mv |
1 |
| dc.relation.citationstartpage.spa.fl_str_mv |
41 |
| dc.relation.citationvolume.spa.fl_str_mv |
15 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Perspectivas en Nutrición Humana |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| dc.rights.uri.*.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
| dc.rights.accessrights.*.fl_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ https://creativecommons.org/licenses/by-nc-sa/2.5/co/ Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad de Antioquia, Escuela de Nutrición y Dietética |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
| institution |
Universidad de Antioquia |
| bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstreams/04848629-3aa9-4f3e-84d8-c3359353200f/download https://bibliotecadigital.udea.edu.co/bitstreams/8a68e4be-3bce-43f6-ae31-4b077ebd46b8/download https://bibliotecadigital.udea.edu.co/bitstreams/95ba7163-6113-4065-a82e-7da84c43bdbf/download https://bibliotecadigital.udea.edu.co/bitstreams/54c83de7-3ede-4e84-b8a5-7ee99b06efff/download https://bibliotecadigital.udea.edu.co/bitstreams/47b38a17-78ce-45e9-8fa0-a81ddf73658f/download https://bibliotecadigital.udea.edu.co/bitstreams/f3dcbdcc-b5e6-4983-b86d-800e300388ee/download https://bibliotecadigital.udea.edu.co/bitstreams/39815fb1-4954-4aa4-9918-384a4405ace5/download |
| bitstream.checksum.fl_str_mv |
f454df89561b13f9582cae2c56d63fe3 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e 8a4605be74aa9ea9d79846c1fba20a33 77efa66b410b49c9065fcc8f06076596 c24dd81e7b5acce11034c871108e963b |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional de la Universidad de Antioquia |
| repository.mail.fl_str_mv |
aplicacionbibliotecadigitalbiblioteca@udea.edu.co |
| _version_ |
1851052542147952640 |
| spelling |
Areiza Mazo, NataliaMaldonado Celis, María ElenaRojano, Benjamín2019-08-06T18:39:00Z2019-08-06T18:39:00Z2013Areiza-Mazo N, Maldonado ME, Rojano B. Extracto acuoso de uchuva (Physalis peruviana): actividades antiproliferativa, apoptótica y antioxidante. Perspect. Nutr. Humana.. 2013;15: 41-55.0124-4108http://hdl.handle.net/10495/116162248-454XABSTRACT: Ethanolic extracts from P. peruviana showed cytotoxic activity against different cancer cell lines. Objective: To evaluate the antiproliferative activity of aqueous extract of the fruit golden berry in colon cancer cells. Materials and Methods: We analyzed cytotoxicity and selectivity index (SI) (MTT), antiproliferation (sulforhodamine-B), apoptosis (cell cycle, Annexin-V, receptors TRAIL-DR4/-DR5 and caspase-3), and antioxidant activity (content of total carotenoids and flavonoids). Results: The aqueous extract showed cytotoxic and antiproliferative effect on SW480 (IC50 = 44.2 µg/ml, SI = 11.6) and SW620 (IC50 = 85.1 µg/ml, SI = 6.0). The hypodiploid SW480 and SW620 cells increased 13% and 12% respectively as well as apoptotic SW480 and SW620 cells (19% and 21 %, respectively), expression of receptors TRAIL-DR4 /-DR5 (SW480: +59% / +53%; SW620: +67% / +65%) and activation of caspase-3. The aqueous extract scavenged OH- radicals, showed low oxygen radical capacity and low scavenging capacity for reactive oxygen and nitrogen species. The total flavonoids and carotenoids contente was 487.1 mg catechin y 0.9 mg β-carotene by 100 g powder, respectively. Conclusions: These findings suggest that golden berry may be a promising source of bioactive compounds with chemopreventive activity against human colon cancer.RESUMEN: Extractos etanólicos de P. peruviana han mostrado actividad citotóxica contra diferentes células cancerosas. Objetivo: estudiar la actividad anticancerígena de un extracto acuoso del fruto uchuva en células de cáncer de colon SW480 y SW620. Materiales y métodos: se analizaron citotoxicidad e índice de selectividad (SI) (MTT), antiproliferación (sulforodamina-B), apoptosis (ciclo celular, anexina-V, receptores TRAIL-DR4/-DR5 y caspasa-3), actividad antioxidante (contenido de flavonoides y carotenoides totales). Resultados: el extracto acuoso de uchuva mostró efecto citotóxico y antiproliferativo en células SW480 (IC50=44,2 µg/mL, SI=11,6) y SW620 (IC50=85,1µg/mL, SI=6,0). Las células hipodiploides SW480 y SW620 aumentaron 13% y 12%, respectivamente. Las células apoptóticas incrementaron 19% y 21% en SW480 y SW620, respectivamente, con incremento en la expresión de receptores TRAIL-DR4/-DR5 en SW480 (+59%/+53%) y SW620 (+67%/+65%), y activación de caspasa-3 en ambas líneas celulares. El extracto neutralizó radicales OH-, presentó baja capacidad reductora y atrapadora de especies reactivas del oxígeno y nitrógeno. El contenido de flavonoides y carotenoides fue 487,1 mg catequina y 0,9 mg β-caroteno por 100 g de liofilizado, respectivamente. Conclusiones: estos hallazgos sugieren que la uchuva puede ser una fuente prometedora de compuestos bioactivos con actividad quimiopreventiva en cáncer de colon humano.application/pdfspaUniversidad de Antioquia, Escuela de Nutrición y DietéticaMedellín, Colombiahttps://creativecommons.org/licenses/by-nc-sa/4.0/https://creativecommons.org/licenses/by-nc-sa/2.5/co/Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Physalis peruvianaCáncer colorrectalColorectal cancerCiclo celularCellular cycleApoptosisAntioxidantesAntioxidantsAnticancerígenosAnti-cancerExtracto acuoso de uchuva (Physalis peruviana) : actividades antiproliferativa, apoptótica y antioxidanteAqueous extract of golden berry (Physalis peruviana) : antiproliferative, apoptotic and antioxidant activitiesArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPerspect. Nutr. Humana.5514115Perspectivas en Nutrición HumanaPublicationORIGINALAreizaNatalia_2013_ExtractoAcuosoUchuva.pdf.pdfAreizaNatalia_2013_ExtractoAcuosoUchuva.pdf.pdfArtículo de investigaciónapplication/pdf1196279https://bibliotecadigital.udea.edu.co/bitstreams/04848629-3aa9-4f3e-84d8-c3359353200f/downloadf454df89561b13f9582cae2c56d63fe3MD51trueAnonymousREADCC-LICENSElicense_urllicense_urltext/plain; charset=utf-849https://bibliotecadigital.udea.edu.co/bitstreams/8a68e4be-3bce-43f6-ae31-4b077ebd46b8/download4afdbb8c545fd630ea7db775da747b2fMD52falseAnonymousREADlicense_textlicense_texttext/html; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstreams/95ba7163-6113-4065-a82e-7da84c43bdbf/downloadd41d8cd98f00b204e9800998ecf8427eMD53falseAnonymousREADlicense_rdflicense_rdfLicenciaapplication/rdf+xml; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstreams/54c83de7-3ede-4e84-b8a5-7ee99b06efff/downloadd41d8cd98f00b204e9800998ecf8427eMD54falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/47b38a17-78ce-45e9-8fa0-a81ddf73658f/download8a4605be74aa9ea9d79846c1fba20a33MD55falseAnonymousREADTEXTAreizaNatalia_2013_ExtractoAcuosoUchuva.pdf.pdf.txtAreizaNatalia_2013_ExtractoAcuosoUchuva.pdf.pdf.txtExtracted texttext/plain36169https://bibliotecadigital.udea.edu.co/bitstreams/f3dcbdcc-b5e6-4983-b86d-800e300388ee/download77efa66b410b49c9065fcc8f06076596MD56falseAnonymousREADTHUMBNAILAreizaNatalia_2013_ExtractoAcuosoUchuva.pdf.pdf.jpgAreizaNatalia_2013_ExtractoAcuosoUchuva.pdf.pdf.jpgGenerated Thumbnailimage/jpeg11124https://bibliotecadigital.udea.edu.co/bitstreams/39815fb1-4954-4aa4-9918-384a4405ace5/downloadc24dd81e7b5acce11034c871108e963bMD57falseAnonymousREAD10495/11616oai:bibliotecadigital.udea.edu.co:10495/116162025-03-27 00:04:01.814https://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |