Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain
ABSTRACT: Candida tropicalis is one of the most pathogenic species within the genus. Increased antifungal resistance has been reported, which is in part due to the organism's ability to form biofilms. In natural products derived from plants, such as essential oils (EOs) or their major component...
- Autores:
-
Zapata Zapata, Carolina
Rojas López, Mauricio
Mesa Arango, Ana Cecilia
Luque, Daniel
Terrón, María C.
Quintero, Wendy
García, Liliana T.
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/43142
- Acceso en línea:
- https://hdl.handle.net/10495/43142
- Palabra clave:
- Candida tropicalis
Aceites Volátiles
Oils, Volatile
Antifúngicos
Antifungal Agents
Sinergismo Farmacológico
Drug Synergism
Lippia origanoides
https://id.nlm.nih.gov/mesh/D041022
https://id.nlm.nih.gov/mesh/D009822
https://id.nlm.nih.gov/mesh/D000935
https://id.nlm.nih.gov/mesh/D004357
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by/4.0/
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| dc.title.spa.fl_str_mv |
Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain |
| title |
Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain |
| spellingShingle |
Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain Candida tropicalis Aceites Volátiles Oils, Volatile Antifúngicos Antifungal Agents Sinergismo Farmacológico Drug Synergism Lippia origanoides https://id.nlm.nih.gov/mesh/D041022 https://id.nlm.nih.gov/mesh/D009822 https://id.nlm.nih.gov/mesh/D000935 https://id.nlm.nih.gov/mesh/D004357 |
| title_short |
Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain |
| title_full |
Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain |
| title_fullStr |
Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain |
| title_full_unstemmed |
Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain |
| title_sort |
Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain |
| dc.creator.fl_str_mv |
Zapata Zapata, Carolina Rojas López, Mauricio Mesa Arango, Ana Cecilia Luque, Daniel Terrón, María C. Quintero, Wendy García, Liliana T. |
| dc.contributor.author.none.fl_str_mv |
Zapata Zapata, Carolina Rojas López, Mauricio Mesa Arango, Ana Cecilia Luque, Daniel Terrón, María C. Quintero, Wendy García, Liliana T. |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo Académico de Epidemiología Clínica Grupo de Inmunología Celular e Inmunogenética |
| dc.subject.decs.none.fl_str_mv |
Candida tropicalis Aceites Volátiles Oils, Volatile Antifúngicos Antifungal Agents Sinergismo Farmacológico Drug Synergism |
| topic |
Candida tropicalis Aceites Volátiles Oils, Volatile Antifúngicos Antifungal Agents Sinergismo Farmacológico Drug Synergism Lippia origanoides https://id.nlm.nih.gov/mesh/D041022 https://id.nlm.nih.gov/mesh/D009822 https://id.nlm.nih.gov/mesh/D000935 https://id.nlm.nih.gov/mesh/D004357 |
| dc.subject.proposal.spa.fl_str_mv |
Lippia origanoides |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D041022 https://id.nlm.nih.gov/mesh/D009822 https://id.nlm.nih.gov/mesh/D000935 https://id.nlm.nih.gov/mesh/D004357 |
| description |
ABSTRACT: Candida tropicalis is one of the most pathogenic species within the genus. Increased antifungal resistance has been reported, which is in part due to the organism's ability to form biofilms. In natural products derived from plants, such as essential oils (EOs) or their major components, there is significant potential to develop new antifungals or to both enhance the efficacy and reduce the toxicity of conventional antifungals. This study aimed to evaluate the effect of combining an EO of Lippia origanoides or thymol with fluconazole on an azole-resistant C. tropicalis strain. Synergism was observed in the combination of fluconazole with the EO and with thymol, and minimal inhibitory concentrations for fluconazole decreased at least 32-fold. As a consequence of the synergistic interactions, mitochondrial membrane potential was reduced, and mitochondrial superoxide production increased. Alteration in nuclear morphology, cell surface, and ultrastructure was also observed. In conclusion, the synergistic interaction between L. origanoides EO or thymol with fluconazole reverted the azole-resistant C. tropicalis phenotype. These findings suggest that L. origanoides EO or thymol alone, or in combination with fluconazole, have the potential for development as antifungal therapies for this yeast, including resistant strains. |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-11-04T19:55:28Z |
| dc.date.available.none.fl_str_mv |
2024-11-04T19:55:28Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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Zapata-Zapata C, Rojas-López M, García LT, Quintero W, Terrón MC, Luque D, Mesa-Arango AC. Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain. J Fungi (Basel). 2023 Aug 30;9(9):888. doi: 10.3390/jof9090888. |
| dc.identifier.issn.none.fl_str_mv |
2309-608X |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/43142 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/jof9090888 |
| identifier_str_mv |
Zapata-Zapata C, Rojas-López M, García LT, Quintero W, Terrón MC, Luque D, Mesa-Arango AC. Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain. J Fungi (Basel). 2023 Aug 30;9(9):888. doi: 10.3390/jof9090888. 2309-608X 10.3390/jof9090888 |
| url |
https://hdl.handle.net/10495/43142 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
J. Fungi. |
| dc.relation.citationendpage.spa.fl_str_mv |
17 |
| dc.relation.citationissue.spa.fl_str_mv |
9 |
| dc.relation.citationstartpage.spa.fl_str_mv |
1 |
| dc.relation.citationvolume.spa.fl_str_mv |
9 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Journal of Fungi |
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https://creativecommons.org/licenses/by/4.0/ |
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17 páginas |
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Zapata Zapata, CarolinaRojas López, MauricioMesa Arango, Ana CeciliaLuque, DanielTerrón, María C.Quintero, WendyGarcía, Liliana T.Grupo Académico de Epidemiología ClínicaGrupo de Inmunología Celular e Inmunogenética2024-11-04T19:55:28Z2024-11-04T19:55:28Z2023Zapata-Zapata C, Rojas-López M, García LT, Quintero W, Terrón MC, Luque D, Mesa-Arango AC. Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain. J Fungi (Basel). 2023 Aug 30;9(9):888. doi: 10.3390/jof9090888.2309-608Xhttps://hdl.handle.net/10495/4314210.3390/jof9090888ABSTRACT: Candida tropicalis is one of the most pathogenic species within the genus. Increased antifungal resistance has been reported, which is in part due to the organism's ability to form biofilms. In natural products derived from plants, such as essential oils (EOs) or their major components, there is significant potential to develop new antifungals or to both enhance the efficacy and reduce the toxicity of conventional antifungals. This study aimed to evaluate the effect of combining an EO of Lippia origanoides or thymol with fluconazole on an azole-resistant C. tropicalis strain. Synergism was observed in the combination of fluconazole with the EO and with thymol, and minimal inhibitory concentrations for fluconazole decreased at least 32-fold. As a consequence of the synergistic interactions, mitochondrial membrane potential was reduced, and mitochondrial superoxide production increased. Alteration in nuclear morphology, cell surface, and ultrastructure was also observed. In conclusion, the synergistic interaction between L. origanoides EO or thymol with fluconazole reverted the azole-resistant C. tropicalis phenotype. These findings suggest that L. origanoides EO or thymol alone, or in combination with fluconazole, have the potential for development as antifungal therapies for this yeast, including resistant strains.Colombia. Ministerio de Ciencia, Tecnología e Innovación - MinCienciasPrograma Ecosistema Científico-Colombia Científica del Fondo Francisco José de CaldasICETEX (Instituto Colombiano de Crédito Educativo y Estudios Técnicos en el Exterior)Colombia. Ministerio de Educación Nacional - MinEducaciónColombia. Ministerio de Comercio, Industria y Turismo - MinCITCOL0008639COL000712117 páginasapplication/pdfengMDPIBasilea, Suizahttps://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis StrainArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionCandida tropicalisAceites VolátilesOils, VolatileAntifúngicosAntifungal AgentsSinergismo FarmacológicoDrug SynergismLippia origanoideshttps://id.nlm.nih.gov/mesh/D041022https://id.nlm.nih.gov/mesh/D009822https://id.nlm.nih.gov/mesh/D000935https://id.nlm.nih.gov/mesh/D004357J. 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