Simulating of microbial growth scale up in a stirred tank bioreactor for aerobic processes using computational fluid dynamics

In this paper, the effects of some empirical scale up criteria (Reynold's number and Impeller power per bioreactor volume) on gas-liquid mass transfer and microbial growth rates are analyzed using computational fluid dynamics simulations in a stirred tank bioreactor. The effects of turbulence, fluid flow and bubbles dynamics were simulated by using a two equation turbulence model, a multiple reference frame and a population balance, respectively. The impeller power per bioreactor volume scale up criterion showed better microbial growth rates than Reynold's number scale up criterion. However, high cellular damage due to turbulence in animal cell culturing are still challenges in large scale up bioreactor prototyping.

• Autores: Niño López, Lilibeth; Gelves Zambrano, Germán Ricardo; Hernandez, S.
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2020
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/45899
• Acceso en línea: https://hdl.handle.net/10495/45899
• Palabra clave: Crecimiento microbiano; Microbial growth; Biorreactores - Dinámica de fluidos; Bioreactors - Fluid dynamics; Técnicas de Cultivo de Célula; Cell Culture Techniques; Número de Reynolds; Reynolds number; Transferencia de masa; Mass transfer; Dinámica de fluidos; Fluid dynamics; Turbulencia; Turbulence; http://id.loc.gov/authorities/subjects/sh85084761; http://id.loc.gov/authorities/subjects/sh88004258; https://id.nlm.nih.gov/mesh/D018929; Biorreactores - Dinámica de fluidos; ODS 9: Industria, innovación e infraestructura. Construir infraestructuras resilientes, promover la industrialización inclusiva y sostenible y fomentar la innovación; ODS 12: Producción y consumo responsables. Garantizar modalidades de consumo y producción sostenibles
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/4.0/