Extracción y purificación de la enzima monóxido de carbono deshidrogenasa a partir de la bacteria carboxidótrofa Oligotropha carboxidovorans
This paper reports the extraction and purification of the carbon monoxide dehydrogenase enzyme from the Oligotropha carboxidovorans bacteria, OM 5 strain. The bacteria grew autotrophically in a mineral medium, in carbon monoxide saturated atmosphere, at 30°C with constant agitation. In order to obta...
- Autores:
-
Cuervo, Raúl A.
Benítez, Neyla
Hurtado, Clara
Bravo, Enrique
Torres, Walter
Larmat, Fernando
- Tipo de recurso:
- Fecha de publicación:
- 2005
- Institución:
- Universidad de San Buenaventura
- Repositorio:
- Repositorio USB
- Idioma:
- spa
- OAI Identifier:
- oai:bibliotecadigital.usb.edu.co:10819/5202
- Acceso en línea:
- http://hdl.handle.net/10819/5202
- Palabra clave:
- Oligotropha carboxidovorans
Extractos enzimáticos
Ecteolla celulosa
Purificación
Oligotropha carboxidovorans
Enzymatic extracts
Ecteola celullose
Purification
Ingeniería agroindustrial
Monóxido de carbono
Bioquímica
- Rights
- License
- Atribución-NoComercial-SinDerivadas 2.5 Colombia
| Summary: | This paper reports the extraction and purification of the carbon monoxide dehydrogenase enzyme from the Oligotropha carboxidovorans bacteria, OM 5 strain. The bacteria grew autotrophically in a mineral medium, in carbon monoxide saturated atmosphere, at 30°C with constant agitation. In order to obtain the crude enzymatic extracts, the crops underwent a breakdown by sonication, solvolysis with non-ionic detergent and centrifugation to eliminate the cellular debris. During the purification process, the crude enzymatic extracts were passed through a molecular exclusion column as well as through an ion-exchange one. |
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