Evaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatis
Tuberculosis (TB) is characterized by a chronic and infectious disease caused by the bacillus Mycobacterium tuberculosis. These types of bacteria mostly infect the lungs, but can spread to bones and meninges; however, there is the possibility of its prevention and treatment. This type of bacteria be...
- Autores:
-
Bustamante Romero, Andrés Felipe
Vásquez Benítez, Sergio David
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/721
- Acceso en línea:
- https://repository.udca.edu.co/handle/11158/721
- Palabra clave:
- Fullereno
Fullerol
Tuberculosis
Hidroxilación
Microtiter
Fulleroles
Mycobacterium
- Rights
- closedAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
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Evaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatis |
| title |
Evaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatis |
| spellingShingle |
Evaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatis Fullereno Fullerol Tuberculosis Hidroxilación Microtiter Fulleroles Mycobacterium |
| title_short |
Evaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatis |
| title_full |
Evaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatis |
| title_fullStr |
Evaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatis |
| title_full_unstemmed |
Evaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatis |
| title_sort |
Evaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatis |
| dc.creator.fl_str_mv |
Bustamante Romero, Andrés Felipe Vásquez Benítez, Sergio David |
| dc.contributor.advisor.spa.fl_str_mv |
Duarte Ruiz, Alvaro, dir. Soto, Carlos Yesid, dir. |
| dc.contributor.author.spa.fl_str_mv |
Bustamante Romero, Andrés Felipe Vásquez Benítez, Sergio David |
| dc.subject.spa.fl_str_mv |
Fullereno Fullerol Tuberculosis Hidroxilación Microtiter |
| topic |
Fullereno Fullerol Tuberculosis Hidroxilación Microtiter Fulleroles Mycobacterium |
| dc.subject.lemb.spa.fl_str_mv |
Fulleroles Mycobacterium |
| description |
Tuberculosis (TB) is characterized by a chronic and infectious disease caused by the bacillus Mycobacterium tuberculosis. These types of bacteria mostly infect the lungs, but can spread to bones and meninges; however, there is the possibility of its prevention and treatment. This type of bacteria becomes resistant to almost all antituberculosis drugs, which makes it difficult to control the disease, which in turn is a motivation for the development of new drugs and treatments. In relation to antibiotic resistance, tuberculosis (TB) can be classified into one of the three main types: drug resistant tb (tb-ds), multidrug resistant tb (mdr-tb), or extensively drug resistant tb- Xdr). On the other hand, the polyhydroxylated derivatives of C60 fullerene have amphipathic and antimicrobial characteristics. Fullerols comprise a functionalized form of fullerene C60, from which biological properties have been determined as a new therapeutic alternative. In this work the antimicrobacterial activity of the polyhydroxylated fullerol derivative on Mycobacterium smegmatis, an environmental mycobacteria, was evaluated by the microdilution technique and microtiter well. The obtained fullerol has a growth inhibitory effect of M. smegmatis at concentrations of 0.5 mg/mL. The compound obtained is characterized by spectroscopic techniques (FT-IR, TGA and UV / VIS), elemental analysis and qualitative methods to dilute its structure. |
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2017 |
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2017-09-13T17:00:47Z |
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2017-09-13T17:00:47Z |
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2017 |
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Trabajo de grado - Pregrado |
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spa |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales https://creativecommons.org/licenses/by-nc/4.0/ Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) http://purl.org/coar/access_right/c_14cb |
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Química Farmacéutica |
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Duarte Ruiz, Alvaro, dir.Soto, Carlos Yesid, dir.Bustamante Romero, Andrés FelipeVásquez Benítez, Sergio David2017-09-13T17:00:47Z2017-09-13T17:00:47Z2017https://repository.udca.edu.co/handle/11158/721Tuberculosis (TB) is characterized by a chronic and infectious disease caused by the bacillus Mycobacterium tuberculosis. These types of bacteria mostly infect the lungs, but can spread to bones and meninges; however, there is the possibility of its prevention and treatment. This type of bacteria becomes resistant to almost all antituberculosis drugs, which makes it difficult to control the disease, which in turn is a motivation for the development of new drugs and treatments. In relation to antibiotic resistance, tuberculosis (TB) can be classified into one of the three main types: drug resistant tb (tb-ds), multidrug resistant tb (mdr-tb), or extensively drug resistant tb- Xdr). On the other hand, the polyhydroxylated derivatives of C60 fullerene have amphipathic and antimicrobial characteristics. Fullerols comprise a functionalized form of fullerene C60, from which biological properties have been determined as a new therapeutic alternative. In this work the antimicrobacterial activity of the polyhydroxylated fullerol derivative on Mycobacterium smegmatis, an environmental mycobacteria, was evaluated by the microdilution technique and microtiter well. The obtained fullerol has a growth inhibitory effect of M. smegmatis at concentrations of 0.5 mg/mL. The compound obtained is characterized by spectroscopic techniques (FT-IR, TGA and UV / VIS), elemental analysis and qualitative methods to dilute its structure.La tuberculosis (TB) se caracteriza por ser una enfermedad crónica e infecciosa causada por el bacilo Mycobacterium tuberculosis. Este tipo de bacterias infectan mayoritariamente los pulmones, pero pueden expandirse a huesos y meninges; sin embargo, existe la posibilidad de su prevención y tratamiento. Este tipo de bacterias se han vuelto resistentes a casi todos los fármacos antituberculosos, lo que dificulta el control de la enfermedad, lo que a su vez es una motivación para el desarrollo de nuevos medicamentos y tratamientos. En relación a la resistencia antibiótica, la tuberculosis (tb) puede clasificarse en uno de los tres tipos principales: tb resistente a fármacos (tb-ds), tb resistente a múltiples fármacos (mdr-tb) o tb extensamente resistente a fármacos (tb-xdr). Por otra parte, los derivados polihidroxilados de fullereno C60 poseen características anfipáticas y antimicrobianas. Los fulleroles comprenden una forma funcionalizada del fullereno C60, de la que se ha determinado propiedades biológicas como una nueva alternativa terapéutica. En este trabajo se evaluó la actividad antimicobacteriana del derivado polihidroxilado fullerol sobre Mycobacterium smegmatis, una micobacteria ambiental, por medio de la técnica de microdilución en pozo o microtiter. El fullerol obtenido exhibe un efecto inhibitorio del crecimiento de M. smegmatis en concentraciones desde 0.5 mg/mL. El compuesto obtenido se caracterizó por medio de técnicas espectroscópicas (FT-IR, TGA y UV/VIS), análisis elemental y métodos cualitativos que permiten dilucidar su estructuraPregradoQuímico(a) FarmacéuticopdfspaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientaleshttps://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/closedAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_14cbFullerenoFullerolTuberculosisHidroxilaciónMicrotiterFullerolesMycobacteriumEvaluación in vitro de la actividad antimicobacteriana de un fullerol C60 (OH) X en Mycobacterium smegmatisTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85Facultad de CienciasQuímica FarmacéuticaPublicationTEXTDOCUMENTO TESIS ANDRES BUSTAMANTE - SERGIO VASQUEZ.pdf.txtDOCUMENTO TESIS ANDRES BUSTAMANTE - SERGIO VASQUEZ.pdf.txtExtracted texttext/plain134446https://repository.udca.edu.co/bitstreams/ec435141-7641-459c-a8a7-5e0c7983123b/downloadd4dea9e09ac8b2dba6e40771cee3ce95MD53THUMBNAILDOCUMENTO TESIS ANDRES BUSTAMANTE - SERGIO VASQUEZ.pdf.jpgDOCUMENTO TESIS ANDRES BUSTAMANTE - SERGIO VASQUEZ.pdf.jpgGenerated Thumbnailimage/jpeg3504https://repository.udca.edu.co/bitstreams/9474c0f4-2cd3-422f-bb83-438b22ec1b6c/downloadf313a0802a20a4e642cbd019169bf354MD54ORIGINALDOCUMENTO TESIS ANDRES BUSTAMANTE - SERGIO VASQUEZ.pdfDOCUMENTO TESIS ANDRES BUSTAMANTE - SERGIO VASQUEZ.pdfapplication/pdf4019525https://repository.udca.edu.co/bitstreams/87c3fd99-2dfe-4444-b089-fb707b5a9d0a/download7c85854446ddf0b8502425c1f87bf75fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repository.udca.edu.co/bitstreams/703267ff-ac48-42aa-a2c8-91dc6071fe38/download8a4605be74aa9ea9d79846c1fba20a33MD5211158/721oai:repository.udca.edu.co:11158/7212024-05-09 14:41:36.395https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientalesopen.accesshttps://repository.udca.edu.coRepositorio - Universidad de Ciencias Aplicadas y Ambientales UDCA.bdigital@metabiblioteca.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 |