Long-term cryostorage of yamú Brycon amazonicus sperm and commercial scale trials

Objective. Determine the effect of cryogenic storage of yamú (Brycon amazonicus) sperm for one year on seminal and biochemical quality variables, as well as, on fertility and hatching under commercial farm conditions. Materials and methods. Four (n=4) mature males of Brycon amazonicus were used to obtain semen, and the fresh sperm (FS) was processed individually using a diluent composed of 5.5% glucose, 12% egg yolk and 10% dimethyl sulfoxide, packed in 5.0 ml macrotubes and cryopreserved in liquid nitrogen vapors. Semen evaluation was performed after cryo-storage for 1 and 12 m. Fertility and hatching tests were carried out on commercial farms using 12 m cryostored semen, FS was used as a control. Results. Semen quality variables such as duration of motility and viability did not show significant differences (p>0.05) between cryostorage times or when compared with FS. The concentration of ATP in FS spermatozoa was the highest, being only significantly different with the cryo-storage time of 1 month. The Total Antioxidant Capacity showed significant differences between treatments (p<0.05), being the highest for FS. Fertility and hatching using semen cryostored for 12 months showed very acceptable values (64.16±5.06% and 50.42±8.10%, respectively). Conclusions. The present work demonstrated the possibility of long-term cryo-storage of Brycon amazonicus sperm, being effective for use under commercial farm conditions.

• Autores: Medina Robles, Victor Mauricio; Barros Barrios, Owens Jose; Guaje, Diana; Suárez-Martínez, Roger Oswaldo; Mira López, Tatiana María; Ramírez Merlano, Juan Antonio
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2025
• Institución: Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
• Repositorio: Repositorio Institucional UDCA
• Idioma: eng
• OAI Identifier: oai:repository.udca.edu.co:11158/6762
• Acceso en línea: https://repository.udca.edu.co/handle/11158/6762; https://doi.org/10.21897/rmvz.3601; https://repository.udca.edu.co/
• Palabra clave: 630 - Agricultura y tecnologías relacionadas::639 - Caza, pesca, conservación, tecnologías relacionadas; Peces; Yamú Brycon amazonicus; Motilidad espermática; Criopreservación; Fertilidad; Especie endémica; Pez de agua dulce
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.es