Evaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLP

Los métodos de identificación bacteriana han representado una necesidad primaria transversal a diversas áreas del desarrollo humano como la medicina (diagnóstico hospitalario, enfermedades nosocomiales), Biología (filogenia y ecología microbiana), agricultura (Bio-fertilizantes, biopesticidas), inoc...

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Autores:
Quintero Ramirez, Nicolás
Tipo de recurso:
Fecha de publicación:
2025
Institución:
Universidad Libre
Repositorio:
RIU - Repositorio Institucional UniLibre
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OAI Identifier:
oai:repository.unilibre.edu.co:10901/30498
Acceso en línea:
https://hdl.handle.net/10901/30498
Palabra clave:
PCR-RFLP
Identificación bacteriana
ARN ribosomal 16S
Bacterias promotoras del crecimiento vegetal
PCR-RFLP
bacterial identification
16S ribosomal RNA
bacterial identification
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openAccess
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/
id RULIBRE2_41ba1bb32165237312b90c06931264af
oai_identifier_str oai:repository.unilibre.edu.co:10901/30498
network_acronym_str RULIBRE2
network_name_str RIU - Repositorio Institucional UniLibre
repository_id_str
dc.title.spa.fl_str_mv Evaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLP
dc.title.alternative.spa.fl_str_mv Standardization of a Protocol for the Identification of Plant Growth-Promoting Bacteria through 16S Ribosomal RNA Gene Analysis Using the PCR-RFLP Technique
title Evaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLP
spellingShingle Evaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLP
PCR-RFLP
Identificación bacteriana
ARN ribosomal 16S
Bacterias promotoras del crecimiento vegetal
PCR-RFLP
bacterial identification
16S ribosomal RNA
bacterial identification
title_short Evaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLP
title_full Evaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLP
title_fullStr Evaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLP
title_full_unstemmed Evaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLP
title_sort Evaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLP
dc.creator.fl_str_mv Quintero Ramirez, Nicolás
dc.contributor.advisor.none.fl_str_mv Siller López, Fernando
Gaviria Arias, Duverney
dc.contributor.author.none.fl_str_mv Quintero Ramirez, Nicolás
dc.subject.spa.fl_str_mv PCR-RFLP
Identificación bacteriana
ARN ribosomal 16S
Bacterias promotoras del crecimiento vegetal
topic PCR-RFLP
Identificación bacteriana
ARN ribosomal 16S
Bacterias promotoras del crecimiento vegetal
PCR-RFLP
bacterial identification
16S ribosomal RNA
bacterial identification
dc.subject.subjectenglish.spa.fl_str_mv PCR-RFLP
bacterial identification
16S ribosomal RNA
bacterial identification
description Los métodos de identificación bacteriana han representado una necesidad primaria transversal a diversas áreas del desarrollo humano como la medicina (diagnóstico hospitalario, enfermedades nosocomiales), Biología (filogenia y ecología microbiana), agricultura (Bio-fertilizantes, biopesticidas), inocuidad alimentaria (patógenos en los alimentos), farmacología (Producción de antibióticos y actividad antimicrobiana de fármacos), entre otros. Entre los métodos mayormente utilizados se encuentran la identificación bacteriana por métodos tradicionales, fenotípicos y genotípicos. Este último consta de diferentes técnicas que, muchas veces, utilizan tecnologías avanzadas de alto costo. No obstante, existen técnicas genotípicas más accesibles para los laboratorios, las cuales requieren de equipos básicos comúnmente encontrados en investigación. El análisis de los polimorfismos de longitud de fragmentos de restricción (PCR-RFLP) pertenece a los métodos basados en la genotipificación por restricción del ADN. Consta del análisis de un gen conservado (reloj molecular) mediante su escisión con endonucleasas, generando perfiles de digestión diferenciales. Normalmente, la técnica es utilizada para diagnosticar la presencia de bacterias de interés particular en una muestra ambiental, sin embargo, no está muy descrita su utilización para la identificación inferencial de bacterias ambientales. El presente estudio pretende evaluar la técnica PCR-RFLP para la identificación de bacterias promotoras del crecimiento vegetal por medio del análisis del ARN ribosomal 16S.
publishDate 2025
dc.date.accessioned.none.fl_str_mv 2025-01-27T17:32:05Z
dc.date.available.none.fl_str_mv 2025-01-27T17:32:05Z
dc.date.created.none.fl_str_mv 2025-01-26
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.local.spa.fl_str_mv Tesis de Pregrado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/bachelorThesis
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10901/30498
url https://hdl.handle.net/10901/30498
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spelling Siller López, FernandoGaviria Arias, DuverneyQuintero Ramirez, NicolásPereira2025-01-27T17:32:05Z2025-01-27T17:32:05Z2025-01-26https://hdl.handle.net/10901/30498Los métodos de identificación bacteriana han representado una necesidad primaria transversal a diversas áreas del desarrollo humano como la medicina (diagnóstico hospitalario, enfermedades nosocomiales), Biología (filogenia y ecología microbiana), agricultura (Bio-fertilizantes, biopesticidas), inocuidad alimentaria (patógenos en los alimentos), farmacología (Producción de antibióticos y actividad antimicrobiana de fármacos), entre otros. Entre los métodos mayormente utilizados se encuentran la identificación bacteriana por métodos tradicionales, fenotípicos y genotípicos. Este último consta de diferentes técnicas que, muchas veces, utilizan tecnologías avanzadas de alto costo. No obstante, existen técnicas genotípicas más accesibles para los laboratorios, las cuales requieren de equipos básicos comúnmente encontrados en investigación. El análisis de los polimorfismos de longitud de fragmentos de restricción (PCR-RFLP) pertenece a los métodos basados en la genotipificación por restricción del ADN. Consta del análisis de un gen conservado (reloj molecular) mediante su escisión con endonucleasas, generando perfiles de digestión diferenciales. Normalmente, la técnica es utilizada para diagnosticar la presencia de bacterias de interés particular en una muestra ambiental, sin embargo, no está muy descrita su utilización para la identificación inferencial de bacterias ambientales. El presente estudio pretende evaluar la técnica PCR-RFLP para la identificación de bacterias promotoras del crecimiento vegetal por medio del análisis del ARN ribosomal 16S.Universidad Libre Seccional Pereira -- Facultad de Ciencias de la Salud, Exactas y Naturales -- MicrobiologíaBacterial identification methods have represented a primary need across various areas of human development, such as medicine (hospital diagnosis, nosocomial diseases), biology (phylogeny and microbial ecology), agriculture (bio-fertilizers, biopesticides), food safety (pathogens in food), pharmacology (production of antibiotics and antimicrobial activity of drugs), among others. The most commonly used methods include bacterial identification through traditional, phenotypic, and genotypic methods. The latter consists of different techniques that often use advanced and costly technologies. However, there are more accessible genotypic techniques for laboratories, which require basic equipment commonly found in research. The analysis of restriction fragment length polymorphisms (PCR-RFLP) belongs to the methods based on DNA restriction genotyping. It involves the analysis of a conserved gene (molecular clock) by cleaving it with endonucleases, generating differential digestion profiles. Usually, the technique is used to diagnose the presence of specific bacteria of interest in an environmental sample. However, its use for inferential identification of environmental bacteria is not well described. This study aims to standardize the PCR-RFLP technique for the identification of plant growth-promoting bacteria through analysis of the 16S ribosomal RNA.PDFhttp://creativecommons.org/licenses/by-nc-nd/2.5/co/Atribución-NoComercial-SinDerivadas 2.5 Colombiainfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PCR-RFLPIdentificación bacterianaARN ribosomal 16SBacterias promotoras del crecimiento vegetalPCR-RFLPbacterial identification16S ribosomal RNAbacterial identificationEvaluación de protocolo para la identificación de bacterias promotoras del crecimiento vegetal mediante el análisis del gen ARN ribosomal 16S con la técnica PCR-RFLPStandardization of a Protocol for the Identification of Plant Growth-Promoting Bacteria through 16S Ribosomal RNA Gene Analysis Using the PCR-RFLP TechniqueTesis de Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1f[1] Paudyal G/ O; P, Paudyal SP, Gupta VNP. 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