Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria

This work aimed to identify the influence of pH, molarity, w/v fraction, extraction time, agitation, and either a sodium (Na2HPO4 ·7H2O-NaH2PO4 ·H2O) or potassium buffer (K2HPO4 -KH2PO4 ) used in the extraction of C-phycoerythrin (C-PE) from a thermotolerant strain of Potamosiphon sp. An experimental design (Minimum Run Resolution V Factorial Design) and a Central Composite Design (CCD) were used. According to the statistical results of the first design, the K-PO4 buffer, pH, molarity, and w/v fraction are vital factors that enhance the extractability of C-PE. The construction of a CCD design of the experiments suggests that the potassium phosphate buffer at pH 5.8, longer extraction times (50 min), and minimal extraction speed (1000 rpm) are ideal for maximizing C-PE concentration, while purity is unaffected by the design conditions. This optimization improves extraction yields and maintains the desired bright purple color of the phycobiliprotein

• Autores: Vergel-Suarez, Ariadna H.; García-Martinez, Janet; López Barrera, German Luciano; Urbina-Suarez, Nestor Andres; Barajas Solano, andres F
• Tipo de recurso: Article of journal
• Fecha de publicación: 2024
• Institución: Universidad Francisco de Paula Santander
• Repositorio: Repositorio Digital UFPS
• Idioma: eng
• OAI Identifier: oai:repositorio.ufps.edu.co:ufps/9149
• Acceso en línea: https://repositorio.ufps.edu.co/handle/ufps/9149
• Palabra clave: cyanobacteria; phycobiliproteins; Natural colorants; biomass dehydration
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/