Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota

395 páginas

Autores:

Tipo de recurso:

Fecha de publicación:: 2022

Institución:: Universidad de la Sabana

Repositorio:: Repositorio Universidad de la Sabana

Idioma:: eng

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spelling	Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiotaPisciculturaProbióticosMicrobioma gastrointestinalBiotecnología acuícolaLactococcus lactis395 páginas409 Stress conditions in intensive aquaculture are generating serious economic loss due to 410 disease outbreaks. For that reason, antibiotics have been used to reduce infection, increase 411 growth rates or even prevent diseases; nevertheless, the application of repeated doses or low 412 concentrations of antibiotics increases the risk of the apparition of antibiotic-resistant bacteria, 413 creating thus the need for alternative biotechnological methods such as probiotic bacteria. 414 Additionally, previous research suggests that bacteria consortia and autochthonous bacteria 415 improve fish health with benefits beyond antibiotics application. These reasons are why this 416 project proposed to determine the probiotic potential of a continuous-flow competitive 417 exclusion culture developed from intestinal microbiota collected from tilapia (O. niloticus) 418 intestinal content, the major cultured fish species in Colombia. The project was divided into 419 three phases; The first phase involved establishing a competitive exclusion culture with 420 antibacterial activity against S. agalactiae. From the last, it was possible to identify that after 421 33 days of incubation of the CFCEC, the more abundant bacteria were from the phyla 422 firmicutes, Lactococcus being the most abundant genus, showing the displacement of 423 opportunistic pathogenic bacteria. The second stage included the in vitro characterization of 424 bacteria isolated from a competitive exclusion culture to identify the probiotic potential; also, a 425 probiotic mixture was designed, incorporated into fish feed, and stabilized in a vacuum package 426 at 4°C. From that, three bacteria were isolated and identified: Lactococcus lactis A12, Priestia 427 megaterium M4, and Priestia sp. M10, a mixture design of the three bacteria, was established 428 for in vivo use. The third phase was the in vivo validation of the fish production.Universidad de La SabanaVillamil Diaz, Luisa MarcelaRuiz Pardo, Ruth YolandaMelo Bolívar, Javier Fernando2022-11-23T14:42:26Z2022-11-23T14:42:26Z2022-10-25Tesis/Trabajo de grado – DoctoradoTesis/Trabajo de grado – Doctoradoinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06info:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfhttps://hdl.handle.net/10818/52744290522TE12112Universidad de La SabanaIntellectum Repositorio Universidad de La SabanaengAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_16ecoai:intellectum.unisabana.edu.co:10818/527442025-10-15T22:11:09Z
dc.title.none.fl_str_mv	Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota
title	Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota
spellingShingle	Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota Piscicultura Probióticos Microbioma gastrointestinal Biotecnología acuícola Lactococcus lactis
title_short	Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota
title_full	Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota
title_fullStr	Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota
title_full_unstemmed	Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota
title_sort	Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota
dc.contributor.none.fl_str_mv	Villamil Diaz, Luisa Marcela Ruiz Pardo, Ruth Yolanda
dc.subject.none.fl_str_mv	Piscicultura Probióticos Microbioma gastrointestinal Biotecnología acuícola Lactococcus lactis
topic	Piscicultura Probióticos Microbioma gastrointestinal Biotecnología acuícola Lactococcus lactis
description	395 páginas
publishDate	2022
dc.date.none.fl_str_mv	2022-11-23T14:42:26Z 2022-11-23T14:42:26Z 2022-10-25
dc.type.none.fl_str_mv	Tesis/Trabajo de grado – Doctorado Tesis/Trabajo de grado – Doctorado info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_db06 info:eu-repo/semantics/doctoralThesis
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/10818/52744 290522 TE12112
url	https://hdl.handle.net/10818/52744
identifier_str_mv	290522 TE12112
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_16ec
rights_invalid_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_16ec
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Universidad de La Sabana
publisher.none.fl_str_mv	Universidad de La Sabana
dc.source.none.fl_str_mv	Universidad de La Sabana Intellectum Repositorio Universidad de La Sabana
institution	Universidad de la Sabana
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Evaluation of the probiotic potential of a competitive exclusion culture from 2 tilapia (Oreochromis niloticus) intestinal microbiota

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