Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum

50 Páginas.

Autores:

Tipo de recurso:

Fecha de publicación:: 2016

Institución:: Universidad de la Sabana

Repositorio:: Repositorio Universidad de la Sabana

Idioma:: spa

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oai_identifier_str	oai:intellectum.unisabana.edu.co:10818/27994
network_acronym_str	REPOUSABAN
network_name_str	Repositorio Universidad de la Sabana
repository_id_str
dc.title.none.fl_str_mv	Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum
title	Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum
spellingShingle	Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum Espuma Liofilización Soluciones (Química) Electroforesis
title_short	Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum
title_full	Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum
title_fullStr	Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum
title_full_unstemmed	Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum
title_sort	Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum
dc.contributor.none.fl_str_mv	Jiménez Junca, Carlos Alberto Prieto Correa, Rosa Erlide
dc.subject.none.fl_str_mv	Espuma Liofilización Soluciones (Química) Electroforesis
topic	Espuma Liofilización Soluciones (Química) Electroforesis
description	50 Páginas.
publishDate	2016
dc.date.none.fl_str_mv	2016-11-04T13:33:46Z 2016-11-04T13:33:46Z 2016 2016
dc.type.none.fl_str_mv	Tesis/Trabajo de grado - Pregrado http://purl.org/coar/resource_type/c_7a1f http://purl.org/coar/version/c_970fb48d4fbd8a85 Texto info:eu-repo/semantics/bachelorThesis http://purl.org/redcol/resource_type/TP
dc.identifier.none.fl_str_mv	Atanasova, L. (2014). Ecophysiology of Trichoderma in Genomic Perspective. In Biotechnology and biology of trichoderma (pp. 25¿28). Basheva, E. S., Kralchevsky, P. a., Christov, N. C., Danov, K. D., Stoyanov, S. D., Blijdenstein, T. B. J., ¿ Lips, A. (2011). Unique properties of bubbles and foam films stabilized by HFBII hydrophobin. Brown, D. E. (1975). The Effect of Acid pH on the Growth Kinetics of Trichoderma viride, XVII, 1199¿1210. Bureiko, A., Trybala, A., Kovalchuk, N., & Starov, V. (2015). Current applications of foams formed from mixed surfactant-polymer solutions. Advances in Colloid and Interface Science, 222, 670¿677. Burghoff, B. (2012). Foam fractionation applications. Journal of Biotechnology, 161(2), 126¿137 Calonje, M., Bernardo, D., Novaes-Ledieu, M., & García Mendoza, C. (2002). Properties of a hydrophobin isolated from the mycoparasitic fungus Verticillium fungicola. Canadian Journal of Microbiology, 48(11), 1030¿ 1034. Cepero de García, M. C., Restrepo R., S., Franco-Molano, A. E., Cárdenas T., M., & Vargas E., N. (2012). Biologia de Hongos (Primera Ed). Bogotá: Universidad de los Andes. Cicatiello, P., Gravagnuolo, A. M., Gnavi, G., Varese, G. C., & Giardina, P. (2016). Marine fungi as source of new hydrophobins. International Journal of Biological Macromolecules, 92, 1229¿1233. Cox, A. R., Aldred, D. L., & Russell, A. B. (2009). Exceptional stability of food foams using class II hydrophobin HFBII. Food Hydrocolloids, 23, 366¿376. Cox, A. R., Cagnol, F., Russell, A. B., & Izzard, M. J. (2007). Surface properties of class ii hydrophobins from Trichoderma reesei and influence on bubble stability. Langmuir¿: The ACS Journal of Surfaces and Colloids, 23(15), 7995¿8002. Dimitrova, L. M., Petkov, P. V., Kralchevsky, P. A., Stoyanov, S. D., & Pelan, E. G. (2016). Production and characterization of stable foams with fine bubbles from solutions of hydrophobin HFBII and its mixtures with other proteins. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 1¿13 EFE. (2014, December 10). Biotecnología en Colombia. El Tiempo. Fameau, A.-L., & Salonen, A. (2014). Effect of particles and aggregated structures on the foam stability and aging. Comptes Rendus Physique, 15, 748¿760. Gil, J. A., Vargas M., L. J., Florez A., Ó. A., & Tobón Z., G. E. (2009). THE EFFECT OF THE SOLVENT RECRYSTALLIZATION AND PARTICLE SIZE ON WETTABILITY OF IBUPROFEN. Vitae, Revista de La Facultad de Química Farmacéutica, 16, 49¿54. Green, A. J., Littlejohn, K. a., Hooley, P., & Cox, P. W. (2013). Formation and stability of food foams and aerated emulsions: Hydrophobins as novel functional ingredients. Current Opinion in Colloid and Interface Science, 18(4), 292¿301. Haas Jimoh Akanbi, M., Post, E., Meter-Arkema, A., Rink, R., Robillard, G. T., Wang, X., ¿ Scholtmeijer, K. (2010). Use of hydrophobins in formulation of water insoluble drugs for oral administration. Colloids and Surfaces. B, Biointerfaces, 75(2), 526¿31. Hakala, T. J., Metsäjoki, J., Granqvist, N., Milani, R., Szilvay, G. R., Elomaa, O., ¿ Li, F. (2015). Adsorption and lubricating properties of HFBII hydrophobins and diblock copolymer poly(methyl methacrylate-b-sodium acrylate) additives in water-lubricated copper vs. a-C:H contacts. Tribology International, 90, 60¿66. Hektor, H. J., & Scholtmeijer, K. (2005). Hydrophobins: proteins with potential. Current Opinion in Biotechnology, 16(4), 434¿439. Huang, Y., Mijiti, G., Wang, Z., Yu, W., Fan, H., Zhang, R., & Liu, Z. (2015). Functional analysis of the class II hydrophobin gene HFB2-6 from the biocontrol agent Trichoderma asperellum ACCC30536. Microbiological Research, 171, 8¿20. Israelichvili, J. (1991). Thermodynamic Principles of Self-Assembly - Chp. 19. Intermolecular & Surface Forces Jackson, A. M., Whipps, J. M., & Lynch, J. M. (1991). Effects of temperature, pH and water potential on growth of four fungi with disease biocontrol potential. World Journal of Microbiology & Biotechnology, 7(4), 494¿501. Jimenez-Junca, C., Sher, A., Gumy, J.-C., & Niranjan, K. (2015). Production of milk foams by steam injection: The effects of steam pressure and nozzle design. Journal of Food Engineering, 166, 247¿254. Kang, J., Hua, X., Yang, R., Chen, Y., & Yang, H. (2015). Characterization of natural low-methoxyl pectin from sunflower head extracted by sodium citrate and purified by ultrafiltration. Food Chemistry, 180, 98¿105. Khalesi, M., Gebruers, K., & Derdelinckx, G. (2015). Recent Advances in Fungal Hydrophobin Towards Using in Industry. The Protein Journal, 34(4), 243¿255. Khalesi, M., Venken, T., Deckers, S., Winterburn, J., Shokribousjein, Z., Gebruers, K., ¿ Derdelinckx, G. (2013). A novel method for hydrophobin extraction using CO2 foam fractionation system. Industrial Crops and Products, 43, 372¿377. Khondee, N., Tathong, S., Pinyakong, O., Müller, R., Soonglerdsongpha, S., Ruangchainikom, C., ¿ Luepromchai, E. (2015). Lipopeptide biosurfactant production by chitosan-immobilized Bacillus sp. GY19 and their recovery by foam fractionation. Biochemical Engineering Journal, 93, 47¿54. Lee, S., Røn, T., Pakkanen, K. I., & Linder, M. (2015). Hydrophobins as aqueous lubricant additive for a soft sliding contact. Colloids and Surfaces. B, Biointerfaces, 125, 264¿9. Li, X., & Stevenson, P. (2012). Foam fractionation. In Foam engineering (Primera, pp. 307¿330). United Kingdom: John Wiley & Sons. https://hdl.handle.net/10818/27994 262795 TE08639
identifier_str_mv	Atanasova, L. (2014). Ecophysiology of Trichoderma in Genomic Perspective. In Biotechnology and biology of trichoderma (pp. 25¿28). Basheva, E. S., Kralchevsky, P. a., Christov, N. C., Danov, K. D., Stoyanov, S. D., Blijdenstein, T. B. J., ¿ Lips, A. (2011). Unique properties of bubbles and foam films stabilized by HFBII hydrophobin. Brown, D. E. (1975). The Effect of Acid pH on the Growth Kinetics of Trichoderma viride, XVII, 1199¿1210. Bureiko, A., Trybala, A., Kovalchuk, N., & Starov, V. (2015). Current applications of foams formed from mixed surfactant-polymer solutions. Advances in Colloid and Interface Science, 222, 670¿677. Burghoff, B. (2012). Foam fractionation applications. Journal of Biotechnology, 161(2), 126¿137 Calonje, M., Bernardo, D., Novaes-Ledieu, M., & García Mendoza, C. (2002). Properties of a hydrophobin isolated from the mycoparasitic fungus Verticillium fungicola. Canadian Journal of Microbiology, 48(11), 1030¿ 1034. Cepero de García, M. C., Restrepo R., S., Franco-Molano, A. E., Cárdenas T., M., & Vargas E., N. (2012). Biologia de Hongos (Primera Ed). Bogotá: Universidad de los Andes. Cicatiello, P., Gravagnuolo, A. M., Gnavi, G., Varese, G. C., & Giardina, P. (2016). Marine fungi as source of new hydrophobins. International Journal of Biological Macromolecules, 92, 1229¿1233. Cox, A. R., Aldred, D. L., & Russell, A. B. (2009). Exceptional stability of food foams using class II hydrophobin HFBII. Food Hydrocolloids, 23, 366¿376. Cox, A. R., Cagnol, F., Russell, A. B., & Izzard, M. J. (2007). Surface properties of class ii hydrophobins from Trichoderma reesei and influence on bubble stability. Langmuir¿: The ACS Journal of Surfaces and Colloids, 23(15), 7995¿8002. Dimitrova, L. M., Petkov, P. V., Kralchevsky, P. A., Stoyanov, S. D., & Pelan, E. G. (2016). Production and characterization of stable foams with fine bubbles from solutions of hydrophobin HFBII and its mixtures with other proteins. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 1¿13 EFE. (2014, December 10). Biotecnología en Colombia. El Tiempo. Fameau, A.-L., & Salonen, A. (2014). Effect of particles and aggregated structures on the foam stability and aging. Comptes Rendus Physique, 15, 748¿760. Gil, J. A., Vargas M., L. J., Florez A., Ó. A., & Tobón Z., G. E. (2009). THE EFFECT OF THE SOLVENT RECRYSTALLIZATION AND PARTICLE SIZE ON WETTABILITY OF IBUPROFEN. Vitae, Revista de La Facultad de Química Farmacéutica, 16, 49¿54. Green, A. J., Littlejohn, K. a., Hooley, P., & Cox, P. W. (2013). Formation and stability of food foams and aerated emulsions: Hydrophobins as novel functional ingredients. Current Opinion in Colloid and Interface Science, 18(4), 292¿301. Haas Jimoh Akanbi, M., Post, E., Meter-Arkema, A., Rink, R., Robillard, G. T., Wang, X., ¿ Scholtmeijer, K. (2010). Use of hydrophobins in formulation of water insoluble drugs for oral administration. Colloids and Surfaces. B, Biointerfaces, 75(2), 526¿31. Hakala, T. J., Metsäjoki, J., Granqvist, N., Milani, R., Szilvay, G. R., Elomaa, O., ¿ Li, F. (2015). Adsorption and lubricating properties of HFBII hydrophobins and diblock copolymer poly(methyl methacrylate-b-sodium acrylate) additives in water-lubricated copper vs. a-C:H contacts. Tribology International, 90, 60¿66. Hektor, H. J., & Scholtmeijer, K. (2005). Hydrophobins: proteins with potential. Current Opinion in Biotechnology, 16(4), 434¿439. Huang, Y., Mijiti, G., Wang, Z., Yu, W., Fan, H., Zhang, R., & Liu, Z. (2015). Functional analysis of the class II hydrophobin gene HFB2-6 from the biocontrol agent Trichoderma asperellum ACCC30536. Microbiological Research, 171, 8¿20. Israelichvili, J. (1991). Thermodynamic Principles of Self-Assembly - Chp. 19. Intermolecular & Surface Forces Jackson, A. M., Whipps, J. M., & Lynch, J. M. (1991). Effects of temperature, pH and water potential on growth of four fungi with disease biocontrol potential. World Journal of Microbiology & Biotechnology, 7(4), 494¿501. Jimenez-Junca, C., Sher, A., Gumy, J.-C., & Niranjan, K. (2015). Production of milk foams by steam injection: The effects of steam pressure and nozzle design. Journal of Food Engineering, 166, 247¿254. Kang, J., Hua, X., Yang, R., Chen, Y., & Yang, H. (2015). Characterization of natural low-methoxyl pectin from sunflower head extracted by sodium citrate and purified by ultrafiltration. Food Chemistry, 180, 98¿105. Khalesi, M., Gebruers, K., & Derdelinckx, G. (2015). Recent Advances in Fungal Hydrophobin Towards Using in Industry. The Protein Journal, 34(4), 243¿255. Khalesi, M., Venken, T., Deckers, S., Winterburn, J., Shokribousjein, Z., Gebruers, K., ¿ Derdelinckx, G. (2013). A novel method for hydrophobin extraction using CO2 foam fractionation system. Industrial Crops and Products, 43, 372¿377. Khondee, N., Tathong, S., Pinyakong, O., Müller, R., Soonglerdsongpha, S., Ruangchainikom, C., ¿ Luepromchai, E. (2015). Lipopeptide biosurfactant production by chitosan-immobilized Bacillus sp. GY19 and their recovery by foam fractionation. Biochemical Engineering Journal, 93, 47¿54. Lee, S., Røn, T., Pakkanen, K. I., & Linder, M. (2015). Hydrophobins as aqueous lubricant additive for a soft sliding contact. Colloids and Surfaces. B, Biointerfaces, 125, 264¿9. Li, X., & Stevenson, P. (2012). Foam fractionation. In Foam engineering (Primera, pp. 307¿330). United Kingdom: John Wiley & Sons. 262795 TE08639
url	https://hdl.handle.net/10818/27994
dc.language.none.fl_str_mv	spa
language	spa
dc.rights.none.fl_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ restrictedAccess
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rights_invalid_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ restrictedAccess http://purl.org/coar/access_right/c_16ec
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Universidad de La Sabana Ingeniería Química Facultad de Ingeniería
publisher.none.fl_str_mv	Universidad de La Sabana Ingeniería Química Facultad de Ingeniería
dc.source.none.fl_str_mv	Universidad de La Sabana Intellectum Repositorio Universidad de la Sabana
institution	Universidad de la Sabana
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spelling	Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianumEspumaLiofilizaciónSoluciones (Química)Electroforesis50 Páginas.Las hidrofobinas son proteínas anfipáticas producidas por hongos filamentosos y actualmente se reportan como biosurfactantes de elevado potencial industrial para aplicaciones en emulsiones, espumas, biosensores, bioremediación y otras más. Estos son péptidos de bajo peso molecular secretados durante la liberación de propágulos, la adhesión a superficies, interacciones con el hospedero y, en general, en procesos biológicos del hongo que involucran interacciones con interfases. En este proyecto se buscó utilizar hidrofobinas producidas por la cepa Trichoderma harzianum para ser evaluadas como agentes espumantes. Primero, se ajustó el crecimiento del hongo a un modelo logístico variando la concentración de la fuente de carbono y el pH inicial del medio. Con esto se determinó que a pH 7 y concentración de glucosa de 20 y 30 g/L fueron las condiciones más favorables para la esporulación del microorganismo, proceso relacionado a la producción de hidrofobinas clase II. Luego, la concentración de estas proteínas se realizó mediante fraccionamiento por espumado con CO2, seguido de loifilización. Mediante electroforesis SDS-PAGE tris-tricina, se detectaron bandas de proteínas de 7 y 14 kDa que presentan actividad en la interfase debido a que después del fraccionamiento se mantuvieron dichas bandas. Además, se presentó una mayor concentración de bandas en el medio con glucosa de 30 g/L.¿¿Universidad de La SabanaIngeniería QuímicaFacultad de IngenieríaJiménez Junca, Carlos AlbertoPrieto Correa, Rosa ErlideBeltrán Parada, Vivian EstefaníaHuertas Beltrán, Mauricio Andrés2016-11-04T13:33:46Z2016-11-04T13:33:46Z20162016Tesis/Trabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Textoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/redcol/resource_type/TPapplication/pdfAtanasova, L. (2014). Ecophysiology of Trichoderma in Genomic Perspective. In Biotechnology and biology of trichoderma (pp. 25¿28).Basheva, E. S., Kralchevsky, P. a., Christov, N. C., Danov, K. D., Stoyanov, S. D., Blijdenstein, T. B. J., ¿ Lips, A. (2011). Unique properties of bubbles and foam films stabilized by HFBII hydrophobin.Brown, D. E. (1975). The Effect of Acid pH on the Growth Kinetics of Trichoderma viride, XVII, 1199¿1210.Bureiko, A., Trybala, A., Kovalchuk, N., & Starov, V. (2015). Current applications of foams formed from mixed surfactant-polymer solutions. Advances in Colloid and Interface Science, 222, 670¿677.Burghoff, B. (2012). Foam fractionation applications. Journal of Biotechnology, 161(2), 126¿137Calonje, M., Bernardo, D., Novaes-Ledieu, M., & García Mendoza, C. (2002). Properties of a hydrophobin isolated from the mycoparasitic fungus Verticillium fungicola. Canadian Journal of Microbiology, 48(11), 1030¿ 1034.Cepero de García, M. C., Restrepo R., S., Franco-Molano, A. E., Cárdenas T., M., & Vargas E., N. (2012). Biologia de Hongos (Primera Ed). Bogotá: Universidad de los Andes.Cicatiello, P., Gravagnuolo, A. M., Gnavi, G., Varese, G. C., & Giardina, P. (2016). Marine fungi as source of new hydrophobins. International Journal of Biological Macromolecules, 92, 1229¿1233.Cox, A. R., Aldred, D. L., & Russell, A. B. (2009). Exceptional stability of food foams using class II hydrophobin HFBII. Food Hydrocolloids, 23, 366¿376.Cox, A. R., Cagnol, F., Russell, A. B., & Izzard, M. J. (2007). Surface properties of class ii hydrophobins from Trichoderma reesei and influence on bubble stability. Langmuir¿: The ACS Journal of Surfaces and Colloids, 23(15), 7995¿8002.Dimitrova, L. M., Petkov, P. V., Kralchevsky, P. A., Stoyanov, S. D., & Pelan, E. G. (2016). Production and characterization of stable foams with fine bubbles from solutions of hydrophobin HFBII and its mixtures with other proteins. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 1¿13EFE. (2014, December 10). Biotecnología en Colombia. El Tiempo.Fameau, A.-L., & Salonen, A. (2014). Effect of particles and aggregated structures on the foam stability and aging. Comptes Rendus Physique, 15, 748¿760.Gil, J. A., Vargas M., L. J., Florez A., Ó. A., & Tobón Z., G. E. (2009). THE EFFECT OF THE SOLVENT RECRYSTALLIZATION AND PARTICLE SIZE ON WETTABILITY OF IBUPROFEN. Vitae, Revista de La Facultad de Química Farmacéutica, 16, 49¿54.Green, A. J., Littlejohn, K. a., Hooley, P., & Cox, P. W. (2013). Formation and stability of food foams and aerated emulsions: Hydrophobins as novel functional ingredients. Current Opinion in Colloid and Interface Science, 18(4), 292¿301.Haas Jimoh Akanbi, M., Post, E., Meter-Arkema, A., Rink, R., Robillard, G. T., Wang, X., ¿ Scholtmeijer, K. (2010). Use of hydrophobins in formulation of water insoluble drugs for oral administration. Colloids and Surfaces. B, Biointerfaces, 75(2), 526¿31.Hakala, T. J., Metsäjoki, J., Granqvist, N., Milani, R., Szilvay, G. R., Elomaa, O., ¿ Li, F. (2015). Adsorption and lubricating properties of HFBII hydrophobins and diblock copolymer poly(methyl methacrylate-b-sodium acrylate) additives in water-lubricated copper vs. a-C:H contacts. Tribology International, 90, 60¿66.Hektor, H. J., & Scholtmeijer, K. (2005). Hydrophobins: proteins with potential. Current Opinion in Biotechnology, 16(4), 434¿439.Huang, Y., Mijiti, G., Wang, Z., Yu, W., Fan, H., Zhang, R., & Liu, Z. (2015). Functional analysis of the class II hydrophobin gene HFB2-6 from the biocontrol agent Trichoderma asperellum ACCC30536. Microbiological Research, 171, 8¿20.Israelichvili, J. (1991). Thermodynamic Principles of Self-Assembly - Chp. 19. Intermolecular & Surface ForcesJackson, A. M., Whipps, J. M., & Lynch, J. M. (1991). Effects of temperature, pH and water potential on growth of four fungi with disease biocontrol potential. World Journal of Microbiology & Biotechnology, 7(4), 494¿501.Jimenez-Junca, C., Sher, A., Gumy, J.-C., & Niranjan, K. (2015). Production of milk foams by steam injection: The effects of steam pressure and nozzle design. Journal of Food Engineering, 166, 247¿254.Kang, J., Hua, X., Yang, R., Chen, Y., & Yang, H. (2015). Characterization of natural low-methoxyl pectin from sunflower head extracted by sodium citrate and purified by ultrafiltration. Food Chemistry, 180, 98¿105.Khalesi, M., Gebruers, K., & Derdelinckx, G. (2015). Recent Advances in Fungal Hydrophobin Towards Using in Industry. The Protein Journal, 34(4), 243¿255.Khalesi, M., Venken, T., Deckers, S., Winterburn, J., Shokribousjein, Z., Gebruers, K., ¿ Derdelinckx, G. (2013). A novel method for hydrophobin extraction using CO2 foam fractionation system. Industrial Crops and Products, 43, 372¿377.Khondee, N., Tathong, S., Pinyakong, O., Müller, R., Soonglerdsongpha, S., Ruangchainikom, C., ¿ Luepromchai, E. (2015). Lipopeptide biosurfactant production by chitosan-immobilized Bacillus sp. GY19 and their recovery by foam fractionation. Biochemical Engineering Journal, 93, 47¿54.Lee, S., Røn, T., Pakkanen, K. I., & Linder, M. (2015). Hydrophobins as aqueous lubricant additive for a soft sliding contact. Colloids and Surfaces. B, Biointerfaces, 125, 264¿9.Li, X., & Stevenson, P. (2012). Foam fractionation. In Foam engineering (Primera, pp. 307¿330). United Kingdom: John Wiley & Sons.https://hdl.handle.net/10818/27994262795TE08639Universidad de La SabanaIntellectum Repositorio Universidad de la SabanaspaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/restrictedAccesshttp://purl.org/coar/access_right/c_16ecoai:intellectum.unisabana.edu.co:10818/279942025-12-15T17:38:07Z

Caracterización de espumas acuosas estabilizadas con extracto rico en hidrofobinas extraídas de una cepa de Trichoderma harzianum

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