Modificación del gen lipA codificante de la lipasa de Pseudomonas aeruginosa PSA01 dirigida a la alteración de su selectividad hacia ácidos grasos de diferente longitud de cadena
Lipase LipA from Pseudomonas aeruginosa is characterized by hydrolyzing a wide range of esterified fatty acids in triacylglycerols, from those with 4 carbons to those with 20 carbons. In this research, we sought to reduce the enzyme preference towards the hydrolysis of fatty acids of certain chain l...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad de la Sabana
- Repositorio:
- Repositorio Universidad de la Sabana
- Idioma:
- spa
- OAI Identifier:
- oai:intellectum.unisabana.edu.co:10818/67073
- Acceso en línea:
- https://hdl.handle.net/10818/67073
- Palabra clave:
- Lipasa de Pseudomonas aeruginosa
LipA
Error prone PCR
Mutagénesis al azar
- Rights
- License
- Attribution-NonCommercial-NoDerivatives 4.0 Internacional
| Summary: | Lipase LipA from Pseudomonas aeruginosa is characterized by hydrolyzing a wide range of esterified fatty acids in triacylglycerols, from those with 4 carbons to those with 20 carbons. In this research, we sought to reduce the enzyme preference towards the hydrolysis of fatty acids of certain chain length. Chemo selective lipases are interesting industrially because they favor the catalysis of fatty acids of specific chain lengths, with other similar ones in the same molecule, promoting their enrichment or selection and reducing additional purification steps. |
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