Morphology, mechanical strength and degradation of polyhydroxyalkanoate scaffolds
Tissue engineering (TE) seeks to improve the unsatisfactory development of implants and medical procedures to solve bone and cartilage injuries. TE aims at regenerating tissues using cell growth platforms (scaffolds), which may consist of natural polymers such as polyhydroxyalkanoate (PHA). PHA is a...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2018
- Institución:
- Universidad Pedagógica y Tecnológica de Colombia
- Repositorio:
- RiUPTC: Repositorio Institucional UPTC
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uptc.edu.co:001/14218
- Acceso en línea:
- https://revistas.uptc.edu.co/index.php/ingenieria/article/view/8073
https://repositorio.uptc.edu.co/handle/001/14218
- Palabra clave:
- articular cartilage
polyhydroxyalkanoate
scaffolds
tissue engineering
andamios
cartílago articular
ingeniería de tejidos
polihidroxialcanoato
- Rights
- License
- Copyright (c) 2018 Liliana Maria Arroyave-Muñoz, Claudia Patricia Ossa-Orozco
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| dc.title.en-US.fl_str_mv |
Morphology, mechanical strength and degradation of polyhydroxyalkanoate scaffolds |
| dc.title.es-ES.fl_str_mv |
Morfología, resistencia mecánica y degradación de plataformas de polihidroxialcanoato |
| title |
Morphology, mechanical strength and degradation of polyhydroxyalkanoate scaffolds |
| spellingShingle |
Morphology, mechanical strength and degradation of polyhydroxyalkanoate scaffolds articular cartilage polyhydroxyalkanoate scaffolds tissue engineering andamios cartílago articular ingeniería de tejidos polihidroxialcanoato |
| title_short |
Morphology, mechanical strength and degradation of polyhydroxyalkanoate scaffolds |
| title_full |
Morphology, mechanical strength and degradation of polyhydroxyalkanoate scaffolds |
| title_fullStr |
Morphology, mechanical strength and degradation of polyhydroxyalkanoate scaffolds |
| title_full_unstemmed |
Morphology, mechanical strength and degradation of polyhydroxyalkanoate scaffolds |
| title_sort |
Morphology, mechanical strength and degradation of polyhydroxyalkanoate scaffolds |
| dc.subject.en-US.fl_str_mv |
articular cartilage polyhydroxyalkanoate scaffolds tissue engineering |
| topic |
articular cartilage polyhydroxyalkanoate scaffolds tissue engineering andamios cartílago articular ingeniería de tejidos polihidroxialcanoato |
| dc.subject.es-ES.fl_str_mv |
andamios cartílago articular ingeniería de tejidos polihidroxialcanoato |
| description |
Tissue engineering (TE) seeks to improve the unsatisfactory development of implants and medical procedures to solve bone and cartilage injuries. TE aims at regenerating tissues using cell growth platforms (scaffolds), which may consist of natural polymers such as polyhydroxyalkanoate (PHA). PHA is an innovative material useful in medical applications due to its degradation capability and bacterial origin that allows large-scale production and control final properties. In this research, we developed commercial PHA scaffolds using the lyophilization technique with a factorial experimental design. We used dichloromethane as PHA solvent, tergitol as surfactant, and liquid nitrogen (N2) for the freezing process. We characterized the PHA by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA); and the scaffolds by scanning electron microscopy (SEM) and mechanical compression and hydrolysis degradation tests. The characterization of the PHA indicated that the material is a mixture of PHA and polylactic acid (PLA). The results showed a suitable pore distribution for migration of chondrocytes through the scaffold, in addition to a behavior similar to that of the articular cartilage, although it presented lower mechanical strength. Also, the scaffolds displayed mass loss in a non-linear way related to the percentage of PHA present in the sample. In conclusion, PHA scaffolds have a potential use in tissue engineering for restoring articular cartilage. |
| publishDate |
2018 |
| dc.date.accessioned.none.fl_str_mv |
2024-07-05T19:11:36Z |
| dc.date.available.none.fl_str_mv |
2024-07-05T19:11:36Z |
| dc.date.none.fl_str_mv |
2018-05-05 |
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research |
| dc.type.es-ES.fl_str_mv |
investigación |
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info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/version/c_970fb48d4fbd8a376 |
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publishedVersion |
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https://revistas.uptc.edu.co/index.php/ingenieria/article/view/8073 10.19053/01211129.v27.n48.2018.8073 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.uptc.edu.co/handle/001/14218 |
| url |
https://revistas.uptc.edu.co/index.php/ingenieria/article/view/8073 https://repositorio.uptc.edu.co/handle/001/14218 |
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10.19053/01211129.v27.n48.2018.8073 |
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eng |
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eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://revistas.uptc.edu.co/index.php/ingenieria/article/view/8073/6523 https://revistas.uptc.edu.co/index.php/ingenieria/article/view/8073/7189 |
| dc.rights.en-US.fl_str_mv |
Copyright (c) 2018 Liliana Maria Arroyave-Muñoz, Claudia Patricia Ossa-Orozco |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
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http://purl.org/coar/access_right/c_abf293 |
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Copyright (c) 2018 Liliana Maria Arroyave-Muñoz, Claudia Patricia Ossa-Orozco http://purl.org/coar/access_right/c_abf293 http://purl.org/coar/access_right/c_abf2 |
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application/pdf application/xml |
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Universidad Pedagógica y Tecnológica de Colombia |
| dc.source.en-US.fl_str_mv |
Revista Facultad de Ingeniería; Vol. 27 No. 48 (2018); 61-70 |
| dc.source.es-ES.fl_str_mv |
Revista Facultad de Ingeniería; Vol. 27 Núm. 48 (2018); 61-70 |
| dc.source.none.fl_str_mv |
2357-5328 0121-1129 |
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Universidad Pedagógica y Tecnológica de Colombia |
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Repositorio Institucional UPTC |
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repositorio.uptc@uptc.edu.co |
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1839633866237673472 |
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2018-05-052024-07-05T19:11:36Z2024-07-05T19:11:36Zhttps://revistas.uptc.edu.co/index.php/ingenieria/article/view/807310.19053/01211129.v27.n48.2018.8073https://repositorio.uptc.edu.co/handle/001/14218Tissue engineering (TE) seeks to improve the unsatisfactory development of implants and medical procedures to solve bone and cartilage injuries. TE aims at regenerating tissues using cell growth platforms (scaffolds), which may consist of natural polymers such as polyhydroxyalkanoate (PHA). PHA is an innovative material useful in medical applications due to its degradation capability and bacterial origin that allows large-scale production and control final properties. In this research, we developed commercial PHA scaffolds using the lyophilization technique with a factorial experimental design. We used dichloromethane as PHA solvent, tergitol as surfactant, and liquid nitrogen (N2) for the freezing process. We characterized the PHA by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA); and the scaffolds by scanning electron microscopy (SEM) and mechanical compression and hydrolysis degradation tests. The characterization of the PHA indicated that the material is a mixture of PHA and polylactic acid (PLA). The results showed a suitable pore distribution for migration of chondrocytes through the scaffold, in addition to a behavior similar to that of the articular cartilage, although it presented lower mechanical strength. Also, the scaffolds displayed mass loss in a non-linear way related to the percentage of PHA present in the sample. In conclusion, PHA scaffolds have a potential use in tissue engineering for restoring articular cartilage.Actualmente, el desarrollo de implantes o procedimientos médicos para resolver lesiones óseas o cartilaginosas no cumple satisfactoriamente con los requerimientos funcionales del tejido afectado; una solución alternativa es el uso de la ingeniería de tejidos (IT), que busca regenerar el tejido con plataformas de crecimiento celular fabricadas, por ejemplo, con polímeros naturales, como el polihidroxialcanoato (PHA), que permite la reconstrucción del tejido gracias a su capacidad de degradación, y cuyo origen bacteriano permite la producción a gran escala y el control de las propiedades finales. En este proyecto se desarrollaron scaffolds de PHA comercial, mediante la técnica de liofilización, con un diseño experimental factorial, utilizando diclorometano como solvente, Tergitol como surfactante y nitrógeno líquido (N2) como congelante. El PHA se caracterizó con espectroscopia de infrarrojo (FTIR) y análisis termogravimétrico (TGA). Los scaffolds obtenidos se caracterizaron con microscopia electrónica de barrido (SEM), ensayos mecánicos de compresión y ensayos de degradación hidrolítica. Los análisis sobre el PHA indicaron que el material es una mezcla de dicho polímero y ácido poliláctico (PLA). Los scaffolds mostraron una distribución de poros adecuada para la migración de condrocitos a través de ellos y presentaron un comportamiento similar al cartílago articular, pero una menor resistencia mecánica; también se encontró que la pérdida de masa está relacionada con el porcentaje de PHA presente en la muestra de una forma no lineal. En conclusión, los scaffolds de PHA tienen un potencial uso en la ingeniería de tejidos para la restauración de cartílago articular.application/pdfapplication/xmlengengUniversidad Pedagógica y Tecnológica de Colombiahttps://revistas.uptc.edu.co/index.php/ingenieria/article/view/8073/6523https://revistas.uptc.edu.co/index.php/ingenieria/article/view/8073/7189Copyright (c) 2018 Liliana Maria Arroyave-Muñoz, Claudia Patricia Ossa-Orozcohttp://purl.org/coar/access_right/c_abf293http://purl.org/coar/access_right/c_abf2Revista Facultad de Ingeniería; Vol. 27 No. 48 (2018); 61-70Revista Facultad de Ingeniería; Vol. 27 Núm. 48 (2018); 61-702357-53280121-1129articular cartilagepolyhydroxyalkanoatescaffoldstissue engineeringandamioscartílago articularingeniería de tejidospolihidroxialcanoatoMorphology, mechanical strength and degradation of polyhydroxyalkanoate scaffoldsMorfología, resistencia mecánica y degradación de plataformas de polihidroxialcanoatoresearchinvestigacióninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a376http://purl.org/coar/version/c_970fb48d4fbd8a85Arroyave-Muñoz, Liliana MariaOssa-Orozco, Claudia Patricia001/14218oai:repositorio.uptc.edu.co:001/142182025-07-18 11:53:44.344metadata.onlyhttps://repositorio.uptc.edu.coRepositorio Institucional UPTCrepositorio.uptc@uptc.edu.co |