Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado
Figuras, tablas
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2025
- Institución:
- Universidad de Caldas
- Repositorio:
- Repositorio Institucional U. Caldas
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.ucaldas.edu.co:ucaldas/22417
- Acceso en línea:
- https://repositorio.ucaldas.edu.co/handle/ucaldas/22417
- Palabra clave:
- 570 - Biología
1. Ciencias Naturales
Microorganismos
Bioinformática
Biotecnología
Oxford nanopore
Butanol
Biología
Microbiología
- Rights
- License
- https://creativecommons.org/licenses/by/4.0/
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REPOUCALDA |
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Repositorio Institucional U. Caldas |
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| dc.title.none.fl_str_mv |
Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado |
| title |
Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado |
| spellingShingle |
Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado 570 - Biología 1. Ciencias Naturales Microorganismos Bioinformática Biotecnología Oxford nanopore Butanol Biología Microbiología |
| title_short |
Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado |
| title_full |
Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado |
| title_fullStr |
Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado |
| title_full_unstemmed |
Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado |
| title_sort |
Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado |
| dc.contributor.none.fl_str_mv |
ORJUELA RODRÍGUEZ, MARCELA MORALES EDWIN, DAVID |
| dc.subject.none.fl_str_mv |
570 - Biología 1. Ciencias Naturales Microorganismos Bioinformática Biotecnología Oxford nanopore Butanol Biología Microbiología |
| topic |
570 - Biología 1. Ciencias Naturales Microorganismos Bioinformática Biotecnología Oxford nanopore Butanol Biología Microbiología |
| description |
Figuras, tablas |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025-06-13T18:46:22Z 2025-06-13T18:46:22Z 2025-06-13 |
| dc.type.none.fl_str_mv |
Informe de investigación http://purl.org/coar/resource_type/c_7a1f Text info:eu-repo/semantics/report |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_93fc |
| dc.identifier.none.fl_str_mv |
https://repositorio.ucaldas.edu.co/handle/ucaldas/22417 Universidad de Caldas Repositorio Institucional Universidad de Caldas repositorio.ucaldas.edu.co |
| url |
https://repositorio.ucaldas.edu.co/handle/ucaldas/22417 |
| identifier_str_mv |
Universidad de Caldas Repositorio Institucional Universidad de Caldas repositorio.ucaldas.edu.co |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
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https://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional (CC BY 4.0) |
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https://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional (CC BY 4.0) http://purl.org/coar/access_right/c_abf2 |
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Universidad de Caldas Facultad de Ciencias Exactas y Naturales Manizales Biología |
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Universidad de Caldas Facultad de Ciencias Exactas y Naturales Manizales Biología |
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Universidad de Caldas |
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Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado570 - Biología1. Ciencias NaturalesMicroorganismosBioinformáticaBiotecnologíaOxford nanoporeButanolBiologíaMicrobiologíaFiguras, tablasLos residuos orgánicos representan uno de los principales desafíos ambientales y de salud pública en la actualidad, debido a las enormes cantidades que se generan diariamente (FAO, 2021). En particular, los residuos provenientes de plazas de mercado, compuestos principalmente por restos de frutas, verduras, hojas y otros productos vegetales, ya que se descomponen rápidamente. En este contexto, las comunidades microbianas que naturalmente colonizan este tipo de residuos desempeñan un papel fundamental, ya que son capaces de transformar la materia orgánica en productos de valor agregado, como biogás, enzimas, ácidos orgánicos y alcoholes. Este potencial convierte a los residuos orgánicos en una materia prima valiosa para la producción sostenible de compuestos químicos y energéticos renovables. Por lo tanto, el presente trabajo tiene como objetivo diseñar un flujo de trabajo que permita determinar, la composición y abundancia de los microorganismos presentes en los residuos orgánicos generados en plazas de mercado. Para lograr este propósito, se propone realizar una revisión bibliográfica enfocada en la recopilación y análisis de protocolos de laboratorio que aborden aspectos clave como la calidad y cuantificación de ADN, así como su secuenciación utilizando la tecnología de secuenciación de tercera generación de Oxford Nanopore. Además, se buscarán microorganismos con potencial biotecnológico, en especial aquellos capaces de generar alcoholes mediante procesos fermentativos. Particularmente, se espera la presencia de bacterias del género Clostridium, reconocidas por su capacidad para producir alcoholes, como el butanol, a través de procesos fermentativos en residuos orgánicos.Organic waste represents one of the main environmental and public health challenges today, due to the vast amounts generated daily (FAO, 2021). In particular, waste originating from public marketplaces—mainly composed of fruit and vegetable residues, leaves, and other plant-based materials—decomposes rapidly. In this context, microbial communities that naturally colonize this type of waste play a fundamental role, as they are capable of transforming organic matter into value-added products such as biogas, enzymes, organic acids, and alcohols. This potential positions organic waste as a valuable raw material for the sustainable production of chemical and renewable energy compounds. Therefore, the present study aims to design a workflow to determine the composition and abundance of microorganisms present in organic waste generated in public marketplaces. To achieve this objective, a literature review will be conducted focusing on the collection and analysis of laboratory protocols addressing key aspects such as DNA quality assessment, quantification, and sequencing using third-generation sequencing technology provided by Oxford Nanopore. Additionally, microorganisms with biotechnological potential will be identified, particularly those capable of producing alcohols through fermentative processes. In particular, the presence of bacteria from the genus Clostridium is expected, as they are well-known for their ability to produce alcohols such as butanol via fermentation of organic waste.Actividades realizadas durante la pasantía -- Participación de la ejecución de proyectos de investigación del área de biotecnología -- Participación de la formulación de proyectos de investigación -- Actividades de formación -- Logros alcanzados -- Desarrollo de un flujo de trabajo para la caracterización microbiana de residuos orgánicos en plazas de mercado -- Productos generados durante su estanciaPregradoMetodología: revisión sistemática usado PRISMA, tiene como objetivo identificar protocolos de extracción, secuenciación de ADN, softwares para el análisis bioinformático y potenciales microorganismo productores de butanol. Bases de datos usadas: Scopus y Web Of ScienceBiólogo(a)BiotecnologíaUniversidad de CaldasFacultad de Ciencias Exactas y NaturalesManizalesBiologíaORJUELA RODRÍGUEZ, MARCELAMORALES EDWIN, DAVIDBecerra López, Mayerli Alejandra2025-06-13T18:46:22Z2025-06-13T18:46:22Z2025-06-13Informe de investigaciónhttp://purl.org/coar/resource_type/c_7a1fTextinfo:eu-repo/semantics/reporthttp://purl.org/coar/resource_type/c_93fc23 páginasapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttps://repositorio.ucaldas.edu.co/handle/ucaldas/22417Universidad de CaldasRepositorio Institucional Universidad de Caldasrepositorio.ucaldas.edu.cospaAbendroth, C., Latorre-Pérez, A., Porcar, M., Simeonov, C., Luschnig, O., Vilanova, C., & Pascual, J. (2020). Shedding light on biogas: Phototrophic biofilms in anaerobic digesters hold potential for improved biogas production. Systematic https://doi.org/10.1016/j.syapm.2019.126024 and Applied Microbiology, 43(1).Abo, B. O., Gao, M., Wu, C., Zhu, W., & Wang, Q. 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