Early effect of the autologous platelet concentrate on induced osteoarthrosis in rabbits: plasmatic levels of PDGF and TGF-β1 and his relation as biomarkers
The objective of this study was to determinate the plasmatic concentration of the PDGF and TGF-β1 growth factors in rabbits with induced osteoarthrosis by cranial cruciate ligament rupture nd treated with intra-articular autologous platelet concentrate or Ringer’s lactate solution. Fourteen adult ma...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Caldas
- Repositorio:
- Repositorio Institucional U. Caldas
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.ucaldas.edu.co:ucaldas/25466
- Acceso en línea:
- https://repositorio.ucaldas.edu.co/handle/ucaldas/25466
https://doi.org/10.17151/vetzo.2017.11.1.8
- Palabra clave:
- Cranial cruciate ligament
degenerative articular process
growth factors
plasma
regenerative therapy
fatores de crescimento
ligamento cruzado cranial
plasma
processo articular degenerativo
terapia regenerativa
- Rights
- openAccess
- License
- Revista Veterinaria y Zootecnia (On Line) - 2017
| Summary: | The objective of this study was to determinate the plasmatic concentration of the PDGF and TGF-β1 growth factors in rabbits with induced osteoarthrosis by cranial cruciate ligament rupture nd treated with intra-articular autologous platelet concentrate or Ringer’s lactate solution. Fourteen adult male rabbits with an average body mass of 3.0 kg of the New Zealand breed were divided into two groups (I and II). Blood samples were collected before cranial cruciate ligament rupture (moment 1), at substitution moment of the ligament (moment 2) and two weeks after ligament repair (moment 3) and centrifuged to obtain the plasma. The growth factors concentration was evaluated employing ELISA kits specifics for the specie and for growth factors. In autologous platelet concentrate group rabbits, was observed statistically significant difference (P<0.05) in the PDGF levels in moment 3 when compared to the group that received the intra-articular Ringer’s lactate solution. The plasmatic levels of TGF-β1 showed statistically significant difference (P<0.05) in the controlled group when compared moment 1 with moment 2 and moment 3, however no significant difference was observed when compared with the group receiving the autologous concentrate platelets in any of the evaluated moments. It can be concluded, that the PDGF can be used as evaluation parameter of the early effect of the intraarticular autologous platelet concentrate. |
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