Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR

El objetivo del presente estudio es analizar el cariotipo bovino mediante la elaboración del cariograma, idiograma y carioidiograma; además, de identificar las regiones organizadoras de nucléolo (NOR) en bovinos de la especie Bos taurus. Muestras de sangre de hembras bovinas fueron cultivadas en per...

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Fecha de publicación:: 2018

Institución:: Universidad de Caldas

Repositorio:: Repositorio Institucional U. Caldas

Idioma:: spa

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network_acronym_str	REPOUCALDA
network_name_str	Repositorio Institucional U. Caldas
repository_id_str
dc.title.none.fl_str_mv	Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR Cytogenetic Analysis in Bos taurus females: ideogram, karyo-ideogram, Ag-NOR banding
title	Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR
spellingShingle	Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR bandeo cromosómico bovino cariotipo cromosoma cultivo celular chromosome banding bovine karyotype chromosome cell culture
title_short	Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR
title_full	Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR
title_fullStr	Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR
title_full_unstemmed	Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR
title_sort	Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR
dc.subject.none.fl_str_mv	bandeo cromosómico bovino cariotipo cromosoma cultivo celular chromosome banding bovine karyotype chromosome cell culture
topic	bandeo cromosómico bovino cariotipo cromosoma cultivo celular chromosome banding bovine karyotype chromosome cell culture
description	El objetivo del presente estudio es analizar el cariotipo bovino mediante la elaboración del cariograma, idiograma y carioidiograma; además, de identificar las regiones organizadoras de nucléolo (NOR) en bovinos de la especie Bos taurus. Muestras de sangre de hembras bovinas fueron cultivadas en períodos de 66 y 72 horas. El análisis citogenético, incluyó tinción convencional con Giemsa y bandeo Ag-NOR. El número diploide fue 2n = 60 y el número fundamental NF = 58. Para la clasificación cromosómica se halló tanto el índice centromérico (r) como la longitud cromosómica total relativa (% LT). El cariotipo se clasificó en tres grupos: grupo A, con 12 pares de cromosomas telocéntricos de tamaño mediano; grupo B, con 17 cromosomas telocéntricos pequeños y grupo C, con cromosomas sexuales metacéntricos. La tendencia en tamaño y forma fue analizada por medio del idiograma y carioidiograma. Seis NOR fueron identificadas en cuatro cromosomas del grupo A y dos del grupo B.
publishDate	2018
dc.date.none.fl_str_mv	2018-07-01T00:00:00Z 2018-07-01T00:00:00Z 2018-07-01 2025-10-08T21:57:17Z 2025-10-08T21:57:17Z
dc.type.none.fl_str_mv	Artículo de revista http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/resource_type/c_2df8fbb1 Text info:eu-repo/semantics/article Journal article http://purl.org/redcol/resource_type/ART info:eu-repo/semantics/publishedVersion http://purl.org/coar/version/c_970fb48d4fbd8a85
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://repositorio.ucaldas.edu.co/handle/ucaldas/25436 10.17151/vetzo.2018.12.2.2 2011-5415 https://doi.org/10.17151/vetzo.2018.12.2.2
url	https://repositorio.ucaldas.edu.co/handle/ucaldas/25436 https://doi.org/10.17151/vetzo.2018.12.2.2
identifier_str_mv	10.17151/vetzo.2018.12.2.2 2011-5415
dc.language.none.fl_str_mv	spa
language	spa
dc.relation.none.fl_str_mv	21 2 10 12 Revista Veterinaria y Zootecnia (On Line)  Ahmad, I. et al. Screening of breeding bulls of different breeds through karyotyping.Pakistan Vet J, v. 24, n. 4, p. 190-192, 2004.  Barragán, K. Citogenética en cérvidos, con énfasis en Venado de cola Blanca (Odocoileus virginianus). Revista de la Asociación de Veterinarios de Vida Silvestre, v. 1, p. 3-9, 2005.  Biltueva, L. et al. A New Case of an Inherited Reciprocal Translocation in Cattle: rcp(13;26)(q24;q11). Cytogenet and Genome Research, v. 144, n. 3, p. 208-211, 2014.  Canavez, F.C. et al. Genome Sequence and Assembly of Bos indicus. Journal of Heredity, v. 103, n. 3, p. 342-348, 2012.  Carlyle, J.T.; Duncan, H.R. Enfermedades determinadas genéticamente. Citogenética. En: Patología veterinaria. Montevideo, Uruguay: Editorial Hemisferio Sur S.A., 1990. p. 249-286.  DiBerardino, D. et al. International System for Cytogenetic Nomenclature of Domestic Animals (1989). The Second International Conference on Standarization of Domestic Animal Karyotypes. Cytogenet Cell Genet, v. 53, p. 65-79, 1990.  Di Meo, G.P. et al. Chromosome evolution and improved cytogenetic maps of the Y chromosome in cattle, zebu, river buffalo, sheep and goat. Chromosome Research, v. 13, p. 349-355, 2005.  Fechheimer, N.S. Cytogenetics in Animal Production. J Dairy Sci, v. 62, p. 844-853.  Ford, C.E. et al. Proceedings of the First International Conference for the Standardization of Banded Karyotypes of Domestic Animals. Hereditas, v. 92, p. 145- 162, 1980.  Gallagher, D.S. Jr.; Womack, J.E. Chromosome Conservation in the Bovidae.Journal of Heredity, v. 83, p. 287-298, 1992.  Gustavsson, I. From Giessen to Toulouse: 20 years in domestic animal cytogenetics. Genet Sel Evol, v. 23, suppl. 1, p. 9-17, 1991.  Halnan, C.R.E.; Watson, J.I. Y chromosome variants in cattle Bos taurus and Bos indicus. Ann Génét Sél Anim, v. 14, n. 1, p. 1-16, 1982.  Hamerton, J.L. et al. Paris Conference (1971): Standardization in Human Cytogenetics. Cytogenetics, v. 11, p. 313-362, 1972.  Howell, W.M.; Black, D.A. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: 1-step method. Experientia, n. 36, p. 1014-1015, 1980.  Iannuzzi, L. et al. Identification of nucleolus organizer chromosomes and frequency of active NORs in river buffalo (Bubalus bubalis L.). Caryologia, v. 49, n. 1, p. 27-34, 1996.  Iannuzzi, L. The contribution of cytogenetics to the genetic improvement of livestock. Chromosome Res, v. 18, p. 711-755, 2010.  Issa, É.C. et al. Cytogenetic analysis of the y chromosome of native brazilian bovine breeds: Preliminary data. Arch Zootec, v. 58, n. 221, p. 93-101, 2009.  Jantarat, S. et al. Standardized karyotype and idiogram of Thai’s native cattle, Bos indicus (Artiodactyla, Bovidae) by convention staining, G-banding, C-banding and NOR-banding techniques. Thai Journal of Genetics, v. 2, n. 2, p. 164-174, 2009.  Kochneva, M.L.; Zhidenova, A.N. A new case of chromosomal mutation in cattle.Chromosome Res, v. 18, p. 711-755, 2010.  Kochneva, M.I. et al. New Case of Reciprocal Translocation rcp (13; 26) in Cattle. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], n. 6, p. 84-89, 2011.  Levan, A. et al. Nomenclature for centromeric position on chromosomes.Hereditas, v. 52, p. 201-220, 1964.  Marafiga, D.T. et al. Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed. Genetics and Molecular Biology, v. 37, n. 2, p. 375-380, 2014.  Mayr, B.; Gruber, K. Nucleolus organizer regions and heterochromatin in the zebu (Bos indicus L.). Theor Appl Genet, v. 73, p. 832-835, 1987.  Moorhead, P.S. et al. Chromosome preparations of leukocytes cultured from human peripheral blood. Experimental Cell Research, v. 20, p. 613-615, 1960.  Parada, R. et al. Cytogenetic and genetic study of a Y-linked microsatellite polymorphism in Polish Black-and-White cattle breed. Saudi Journal of Biological Sciences, p. 1-5, 2017.  Parma, P. et al. Reciprocal Translocations in Cattle: frequency estimation.Chromosome Res, v. 18, p. 711-755, 2010.  Reig, O. et al. Chromosomal speciation in ressenkreis spiny rats (Genus Proechimys, Rodentia Echimyidae). Genetica, v. 52-53, p. 291-312, 1980.  Sánchez, I.C.A. et al. Introgresión genética de Bos indicus (Bovidae) en bovinos criollos colombianos de origen Bos taurus. Acta Biol Colomb, v. 13, n. 1, p. 131-142, 2008.  Spotorno, A.E. Conceptos y métodos de cariología descriptiva y comparada. En: UNESCO. El núcleo, los cromosomas y la evolución. Santiago de Chile, Chile: Impresores Alfabeta, 1985.  Spotorno, A.E. et al. Chromosome divergence of Octodon lunatus andAbrocoma bennetti and the origins of Octodontoidea (Rodentia: Histricognathi).Revista Chilena de Historia Natural, v. 68, p. 227-239, 1995.  Stranzinger, G.F. et al. Y chromosome polymorphism in various breeds of cattle (Bos taurus) in Switzerland. J Appl Genet, v. 48, n. 3, p. 241-245, 2007.  Trukhachev, V.I. et al. Characteristics of the Chromosome Set of Holstein Cows with Reproductive Disorders in the North-Caucasian Cattle Population. Cytology and Genetics, v. 51, n. 4, p. 272-277, 2017.  Verma, R.S.; Babú, A. Banding techniques. En: Human Chromosomes: Principles and techniques. New York, USA: McGraw-Hill, 1989. p. 45-62. Wurster, D.H.; Benirshcke, K. Chromosome Studies in the Superfamily Bovoidea.Chromosoma, v. 25, p. 152-171, 1968.  Yimer, N.; Rosnina, Y. Chromosomal Anomalies and Infertility in Farm Animals: A Review. Pertanika J Trop Agric Sci, v. 37, n. 1, p. 1-18, 2014. Núm. 2 , Año 2018 : Julio - Diciembre https://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/download/3275/3041
dc.rights.none.fl_str_mv	Revista Veterinaria y Zootecnia (On Line) - 2018 https://creativecommons.org/licenses/by-nc-sa/4.0/ info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv	Revista Veterinaria y Zootecnia (On Line) - 2018 https://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Universidad de Caldas
publisher.none.fl_str_mv	Universidad de Caldas
dc.source.none.fl_str_mv	https://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/view/3275
institution	Universidad de Caldas
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spelling	Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NORCytogenetic Analysis in Bos taurus females: ideogram, karyo-ideogram, Ag-NOR bandingbandeo cromosómicobovinocariotipocromosomacultivo celularchromosome bandingbovinekaryotypechromosomecell cultureEl objetivo del presente estudio es analizar el cariotipo bovino mediante la elaboración del cariograma, idiograma y carioidiograma; además, de identificar las regiones organizadoras de nucléolo (NOR) en bovinos de la especie Bos taurus. Muestras de sangre de hembras bovinas fueron cultivadas en períodos de 66 y 72 horas. El análisis citogenético, incluyó tinción convencional con Giemsa y bandeo Ag-NOR. El número diploide fue 2n = 60 y el número fundamental NF = 58. Para la clasificación cromosómica se halló tanto el índice centromérico (r) como la longitud cromosómica total relativa (% LT). El cariotipo se clasificó en tres grupos: grupo A, con 12 pares de cromosomas telocéntricos de tamaño mediano; grupo B, con 17 cromosomas telocéntricos pequeños y grupo C, con cromosomas sexuales metacéntricos. La tendencia en tamaño y forma fue analizada por medio del idiograma y carioidiograma. Seis NOR fueron identificadas en cuatro cromosomas del grupo A y dos del grupo B.The objective of the present study is to analyze the bovine karyotype through the elaboration of the karyogram, ideogram and karyoideogram, in addition to identifying the Nuclear Organizer Regions (NORs) in cattle of the species Bos taurus. Female cattle blood samples were cultured in periods of 66 and 72 hours. The cytogenetic analysis included conventional Giemsa staining and banding Ag-NOR. The diploid number was 2n = 60 and the fundamental number FN = 58. For the chromosomal classification, both the centromeric index (r) and total chromosomal relative length (TL %) were found. The karyotype was classified into three groups, group A with 12 pairs of medium size telocentric chromosomes, group B with 17 small telocentric chromosomes, and group C with metacentric sex chromosomes. The trend in size and shape was analyzed by means of the ideogram and the karyoideogram. Six NORs were identified in four chromosomes from group A and two from Group B.Universidad de Caldas2018-07-01T00:00:00Z2025-10-08T21:57:17Z2018-07-01T00:00:00Z2025-10-08T21:57:17Z2018-07-01Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articleJournal articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85application/pdfhttps://repositorio.ucaldas.edu.co/handle/ucaldas/2543610.17151/vetzo.2018.12.2.22011-5415https://doi.org/10.17151/vetzo.2018.12.2.2https://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/view/3275spa2121012Revista Veterinaria y Zootecnia (On Line) Ahmad, I. et al. Screening of breeding bulls of different breeds through karyotyping.Pakistan Vet J, v. 24, n. 4, p. 190-192, 2004. Barragán, K. Citogenética en cérvidos, con énfasis en Venado de cola Blanca (Odocoileus virginianus). Revista de la Asociación de Veterinarios de Vida Silvestre, v. 1, p. 3-9, 2005. Biltueva, L. et al. A New Case of an Inherited Reciprocal Translocation in Cattle: rcp(13;26)(q24;q11). Cytogenet and Genome Research, v. 144, n. 3, p. 208-211, 2014. Canavez, F.C. et al. Genome Sequence and Assembly of Bos indicus. Journal of Heredity, v. 103, n. 3, p. 342-348, 2012. Carlyle, J.T.; Duncan, H.R. Enfermedades determinadas genéticamente. Citogenética. En: Patología veterinaria. Montevideo, Uruguay: Editorial Hemisferio Sur S.A., 1990. p. 249-286. DiBerardino, D. et al. International System for Cytogenetic Nomenclature of Domestic Animals (1989). The Second International Conference on Standarization of Domestic Animal Karyotypes. Cytogenet Cell Genet, v. 53, p. 65-79, 1990. Di Meo, G.P. et al. Chromosome evolution and improved cytogenetic maps of the Y chromosome in cattle, zebu, river buffalo, sheep and goat. Chromosome Research, v. 13, p. 349-355, 2005. Fechheimer, N.S. Cytogenetics in Animal Production. J Dairy Sci, v. 62, p. 844-853. Ford, C.E. et al. Proceedings of the First International Conference for the Standardization of Banded Karyotypes of Domestic Animals. Hereditas, v. 92, p. 145- 162, 1980. Gallagher, D.S. Jr.; Womack, J.E. Chromosome Conservation in the Bovidae.Journal of Heredity, v. 83, p. 287-298, 1992. Gustavsson, I. From Giessen to Toulouse: 20 years in domestic animal cytogenetics. Genet Sel Evol, v. 23, suppl. 1, p. 9-17, 1991. Halnan, C.R.E.; Watson, J.I. Y chromosome variants in cattle Bos taurus and Bos indicus. Ann Génét Sél Anim, v. 14, n. 1, p. 1-16, 1982. Hamerton, J.L. et al. Paris Conference (1971): Standardization in Human Cytogenetics. Cytogenetics, v. 11, p. 313-362, 1972. Howell, W.M.; Black, D.A. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: 1-step method. Experientia, n. 36, p. 1014-1015, 1980. Iannuzzi, L. et al. Identification of nucleolus organizer chromosomes and frequency of active NORs in river buffalo (Bubalus bubalis L.). Caryologia, v. 49, n. 1, p. 27-34, 1996. Iannuzzi, L. The contribution of cytogenetics to the genetic improvement of livestock. Chromosome Res, v. 18, p. 711-755, 2010. Issa, É.C. et al. Cytogenetic analysis of the y chromosome of native brazilian bovine breeds: Preliminary data. Arch Zootec, v. 58, n. 221, p. 93-101, 2009. Jantarat, S. et al. Standardized karyotype and idiogram of Thai’s native cattle, Bos indicus (Artiodactyla, Bovidae) by convention staining, G-banding, C-banding and NOR-banding techniques. Thai Journal of Genetics, v. 2, n. 2, p. 164-174, 2009. Kochneva, M.L.; Zhidenova, A.N. A new case of chromosomal mutation in cattle.Chromosome Res, v. 18, p. 711-755, 2010. Kochneva, M.I. et al. New Case of Reciprocal Translocation rcp (13; 26) in Cattle. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], n. 6, p. 84-89, 2011. Levan, A. et al. Nomenclature for centromeric position on chromosomes.Hereditas, v. 52, p. 201-220, 1964. Marafiga, D.T. et al. Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed. Genetics and Molecular Biology, v. 37, n. 2, p. 375-380, 2014. Mayr, B.; Gruber, K. Nucleolus organizer regions and heterochromatin in the zebu (Bos indicus L.). Theor Appl Genet, v. 73, p. 832-835, 1987. Moorhead, P.S. et al. Chromosome preparations of leukocytes cultured from human peripheral blood. Experimental Cell Research, v. 20, p. 613-615, 1960. Parada, R. et al. Cytogenetic and genetic study of a Y-linked microsatellite polymorphism in Polish Black-and-White cattle breed. Saudi Journal of Biological Sciences, p. 1-5, 2017. Parma, P. et al. Reciprocal Translocations in Cattle: frequency estimation.Chromosome Res, v. 18, p. 711-755, 2010. Reig, O. et al. Chromosomal speciation in ressenkreis spiny rats (Genus Proechimys, Rodentia Echimyidae). Genetica, v. 52-53, p. 291-312, 1980. Sánchez, I.C.A. et al. Introgresión genética de Bos indicus (Bovidae) en bovinos criollos colombianos de origen Bos taurus. Acta Biol Colomb, v. 13, n. 1, p. 131-142, 2008. Spotorno, A.E. Conceptos y métodos de cariología descriptiva y comparada. En: UNESCO. El núcleo, los cromosomas y la evolución. Santiago de Chile, Chile: Impresores Alfabeta, 1985. Spotorno, A.E. et al. Chromosome divergence of Octodon lunatus andAbrocoma bennetti and the origins of Octodontoidea (Rodentia: Histricognathi).Revista Chilena de Historia Natural, v. 68, p. 227-239, 1995. Stranzinger, G.F. et al. Y chromosome polymorphism in various breeds of cattle (Bos taurus) in Switzerland. J Appl Genet, v. 48, n. 3, p. 241-245, 2007. Trukhachev, V.I. et al. Characteristics of the Chromosome Set of Holstein Cows with Reproductive Disorders in the North-Caucasian Cattle Population. Cytology and Genetics, v. 51, n. 4, p. 272-277, 2017. Verma, R.S.; Babú, A. Banding techniques. En: Human Chromosomes: Principles and techniques. New York, USA: McGraw-Hill, 1989. p. 45-62. Wurster, D.H.; Benirshcke, K. Chromosome Studies in the Superfamily Bovoidea.Chromosoma, v. 25, p. 152-171, 1968. Yimer, N.; Rosnina, Y. Chromosomal Anomalies and Infertility in Farm Animals: A Review. Pertanika J Trop Agric Sci, v. 37, n. 1, p. 1-18, 2014.Núm. 2 , Año 2018 : Julio - Diciembrehttps://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/download/3275/3041Revista Veterinaria y Zootecnia (On Line) - 2018https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Clavijo-Maldonado, AlejandroGonzalez-Aguirre, Laura EstefaníaRodríguez-Valencia, AlonsoRamos-Salazar, Cesar Augustooai:repositorio.ucaldas.edu.co:ucaldas/254362025-10-08T21:57:17Z

Análisis citogenético en hembras Bos taurus: idiograma, carioidiograma y bandas Ag-NOR

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