Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry

Antecedentes: las diferentes etapas que conforman el análisis de acilcarnitinas por espectrometría de masas en tándem, tales como extracción, derivatización, inyección y otras, pueden ser influenciadas por algunos factores técnicos incrementando o disminuyendo los niveles de detección. Objetivo: el...

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2022
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Universidad de Caldas
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Repositorio Institucional U. Caldas
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eng
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oai:repositorio.ucaldas.edu.co:ucaldas/23737
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https://repositorio.ucaldas.edu.co/handle/ucaldas/23737
https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5509
Palabra clave:
carnitina
acilcarnitinas
espectrometría de masas en tándem
MS/MS
tandem mass spectrometry
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openAccess
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https://creativecommons.org/licenses/by-nc-sa/4.0/
id REPOUCALDA_7d5aae0f2e834c6179f282b2b2a0e6b2
oai_identifier_str oai:repositorio.ucaldas.edu.co:ucaldas/23737
network_acronym_str REPOUCALDA
network_name_str Repositorio Institucional U. Caldas
repository_id_str
dc.title.none.fl_str_mv Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry
Efecto de las concentraciones de acetonitrilo en la determinación de acilcarnitinas mediante espectrometría de masas en tándem.
title Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry
spellingShingle Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry
carnitina
acilcarnitinas
espectrometría de masas en tándem
MS/MS
tandem mass spectrometry
title_short Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry
title_full Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry
title_fullStr Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry
title_full_unstemmed Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry
title_sort Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry
dc.subject.none.fl_str_mv carnitina
acilcarnitinas
espectrometría de masas en tándem
MS/MS
tandem mass spectrometry
topic carnitina
acilcarnitinas
espectrometría de masas en tándem
MS/MS
tandem mass spectrometry
description Antecedentes: las diferentes etapas que conforman el análisis de acilcarnitinas por espectrometría de masas en tándem, tales como extracción, derivatización, inyección y otras, pueden ser influenciadas por algunos factores técnicos incrementando o disminuyendo los niveles de detección. Objetivo: el presente estudio evaluó el efecto de la concentración del acetonitrilo utilizado durante la determinación de acilcarnitinas en sangre, sobre la sensibilidad obtenida durante el análisis. Metodología: antes del análisis de acilcarnitinas por espectrometría de masas en tándem, las muestras fueron disueltas en diferentes disoluciones de acetonitrilo en agua [50, 60, 70, 80, 90, and 100% (v/v)] y cada espécimen de sangre fue procesado 5 veces para cada dilución. Resultados: se observó que a medida que se incrementan los niveles de acetonitrilo, la intensidad de los picos de acilcarnitinas se incrementan significativamente. Sin embargo, a las concentraciones más altas de acetonitrilo, éste reacciona con el poliestireno de las bandejas, apareciendo picos en el perfil de acilcarnitinas, específicamente a m/z 302. Conclusión: una mezcla al 70% v/v acetonitrilo/ agua, se recomienda para usar como solvente óptimo, para la disolución de los extractos antes del análisis de acilcarnitinas en sangre mediante espectrometría de masas en tándem.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-17T00:37:08Z
2022-03-17T00:37:08Z
2022-03-17
2025-10-08T21:16:36Z
2025-10-08T21:16:36Z
dc.type.none.fl_str_mv Artículo de revista
http://purl.org/coar/resource_type/c_6501
http://purl.org/coar/resource_type/c_2df8fbb1
Text
info:eu-repo/semantics/article
Journal article
http://purl.org/redcol/resource_type/ART
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/version/c_970fb48d4fbd8a85
status_str publishedVersion
dc.identifier.none.fl_str_mv 1657-9550
https://repositorio.ucaldas.edu.co/handle/ucaldas/23737
2462-960X
https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5509
identifier_str_mv 1657-9550
2462-960X
url https://repositorio.ucaldas.edu.co/handle/ucaldas/23737
https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5509
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 16
1
9
9
Biosalud
Rose ME, Johnstone RAW. Mass spectrometry for chemists and biochemists. Cambridge: Cambridge University Press; 1982. p. 1-148.
Wilson K. Mass spectrometric techniques. In: Wilson K, Walker J, eds. Practical Biochemistry. 4 ed. Cambridge: Cambridge University Press. 1994. p. 381-424.
Harrata AK, Domelsmith LN, Cole RB. Electrospray mass spectrometry for characterization of lipid A from Enterobacter agglomerans. Biol Mass Spectrom 1993;22:59-67.
Niwa T. Basic theory of mass spectrometry. Clin Chim Acta 1995; 241-242:15-71.
McClellan JE, Murphy JP, Mulholland J, Yost RA. Effects of fragile ions on mass resolution and on isolation for tandem mass spectrometry in the quadrupole ion trap mass spectrometer. Anal Chem 2002;74:402-412.
Johnstone RAW, Rose ME. Mass spectrometry for chemist and biochemist. 2 ed. Cambridge: Cambridge University Press; 1994.
Millington DS, Kodo N, Norwood DL, Roe CR. Tandem Mass Spectrometry: A new method for acylcarnitine profiling with potential for neonatal screening for inborn errors of metabolism. J Inher Metab Dis 1990;13:321-324.
Johnson JV, Lee MS, Lee MR, Yost T. Triple Quadrupole MS/MS in Biomedical Research. Mass Spectrometry in Biomedical Research. 2 ed. New York: Plenum Press; 1986. Chapter 25:459-457.
Millington DS, Chace DH. Carnitine and acylcarnitines in metabolic disease diagnosis and management. In: Desiderio DM, ed. Mass spectrometry: clinical and biomedical applications. Vol. I. New York: Plenum Press; 1992. p. 299-316.
Libert R, Van Hoof F, Thillaye M, Vincent MF, Nassogne MC, Stroobant V, et al. Identification of new medium-chain acylcarnitines present in urine of a patient with medium-chain acyl-CoA dehydrogenase deficiency. J Inher Metab Dis1999;22:9-18.
Shigmatsu Y, Hata I, Nakai A, Kikawa Y, Sudo M, Tanaka Y, et al. Prenatal Diagnosis of Organic Acidemias Based on Amniotic Fluid Levels of Acylcarnitines. Pediatr Res 1996;39:680-684.
Rashed MS, Ozand PT, Bennet MJ, Barnard JJ, Govindaraza DR, Rinaldo P. Inborn errors of metabolism diagnosed in sudden death cases by acylcarnitine analysis of postmorten bile. Clin Chem 1995;41:1109-1114.
Millington DS, Norwood DL, Kodo N, Roe CR, Inoue F. Application of fast atom bombardment with tandem mass spectrometry and liquid chromatography/mass spectrometry to the analysis of acylcarnitines in human urine, blood, and tissue. Anal Biochem 1989;180(2):331-9.
Stevens RD, Hillman SL, Worthy S, Sanders D, Millington DS. Assay for free and total carnitine in human plasma using tandem mass spectrometry. Clin Chem 2000;46(5):727-9.
Kodo N, Millington DS, Norwood DL, Roe CR. Quantitative assay of free and total carnitine using tandem mass spectrometry. Clin Chim Acta 1990;186(3):383-90.
Lepage N, Aucoin S. Measurement of plasma/serum acylcarnitines using tandem mass spectrometry. Methods Mol Biol 2010;603:9-25.
Osorio JH, Pourfarzam M. Hydrolysis of acylcarnitines during measurement in blood and plasma by tandem mass spectrometry. Acta Bioquim Clin Latinoam 2010;44(2):15-20.
Budd MA, Tanaka K, Holmes LM, Efron ML, Crawford JD, Isselbacher KJ. Isovalefic acidemia: clinical features of a new genetic defect of leucine metabolism. N Engl J Med 1967;277:321-4.
Osorio JH, Pourfarzam M. Early diagnosis of neurometabolic diseases by tandem mass spectrometry. Cord blood acylcarnitine profile. Rev Neurol 2004;38:11-16.
Núm. 1 , Año 2010 : Enero - Junio
https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/download/5509/4975
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
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dc.publisher.none.fl_str_mv Universidad de Caldas
publisher.none.fl_str_mv Universidad de Caldas
dc.source.none.fl_str_mv https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5509
institution Universidad de Caldas
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometryEfecto de las concentraciones de acetonitrilo en la determinación de acilcarnitinas mediante espectrometría de masas en tándem.carnitinaacilcarnitinasespectrometría de masas en tándemMS/MStandem mass spectrometryAntecedentes: las diferentes etapas que conforman el análisis de acilcarnitinas por espectrometría de masas en tándem, tales como extracción, derivatización, inyección y otras, pueden ser influenciadas por algunos factores técnicos incrementando o disminuyendo los niveles de detección. Objetivo: el presente estudio evaluó el efecto de la concentración del acetonitrilo utilizado durante la determinación de acilcarnitinas en sangre, sobre la sensibilidad obtenida durante el análisis. Metodología: antes del análisis de acilcarnitinas por espectrometría de masas en tándem, las muestras fueron disueltas en diferentes disoluciones de acetonitrilo en agua [50, 60, 70, 80, 90, and 100% (v/v)] y cada espécimen de sangre fue procesado 5 veces para cada dilución. Resultados: se observó que a medida que se incrementan los niveles de acetonitrilo, la intensidad de los picos de acilcarnitinas se incrementan significativamente. Sin embargo, a las concentraciones más altas de acetonitrilo, éste reacciona con el poliestireno de las bandejas, apareciendo picos en el perfil de acilcarnitinas, específicamente a m/z 302. Conclusión: una mezcla al 70% v/v acetonitrilo/ agua, se recomienda para usar como solvente óptimo, para la disolución de los extractos antes del análisis de acilcarnitinas en sangre mediante espectrometría de masas en tándem.Background: the several steps for acylcarnitine analysis by tandem mass spectrometry such as extraction, derivatisation, injection and others, can be influenced by some technical factors improving or impairing the levels of detection. Objective: the present study evaluated the effect of different concentrations of the acetonitrile used during blood acylcarnitines measurement on the sensitivity obtained during the analysis. Methodology: prior to acylcarnitine analysis by tandem mass spectrometry, samples were re-dissolved in different acetonitrile in water dilutions [50, 60, 70, 80, 90, and 100% (v/v)] and each single blood specimen was processed five times for each dilution. Results: it was observed that by increasing the concentration of acetonitrile the intensity of acylcarnitine peaks significantly increased. However at the highest concentrations of acetonitrile, this reacted with the polystyrene material of the microtitre plates, and resulted in peaks appearing in acylcarnitine profiles, specifically at m/z 302. Conclusion: a mixture of 70% v/v acetonitrile/water is recommended to use as optimum solvent to dissolve the extracts prior to blood acylcarnitine analysis by tandem mass spectrometry.Universidad de Caldas2022-03-17T00:37:08Z2025-10-08T21:16:36Z2022-03-17T00:37:08Z2025-10-08T21:16:36Z2022-03-17Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articleJournal articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85application/pdf1657-9550https://repositorio.ucaldas.edu.co/handle/ucaldas/237372462-960Xhttps://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5509https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5509eng16199BiosaludRose ME, Johnstone RAW. Mass spectrometry for chemists and biochemists. Cambridge: Cambridge University Press; 1982. p. 1-148.Wilson K. Mass spectrometric techniques. In: Wilson K, Walker J, eds. Practical Biochemistry. 4 ed. Cambridge: Cambridge University Press. 1994. p. 381-424.Harrata AK, Domelsmith LN, Cole RB. Electrospray mass spectrometry for characterization of lipid A from Enterobacter agglomerans. Biol Mass Spectrom 1993;22:59-67.Niwa T. Basic theory of mass spectrometry. Clin Chim Acta 1995; 241-242:15-71.McClellan JE, Murphy JP, Mulholland J, Yost RA. Effects of fragile ions on mass resolution and on isolation for tandem mass spectrometry in the quadrupole ion trap mass spectrometer. Anal Chem 2002;74:402-412.Johnstone RAW, Rose ME. Mass spectrometry for chemist and biochemist. 2 ed. Cambridge: Cambridge University Press; 1994.Millington DS, Kodo N, Norwood DL, Roe CR. Tandem Mass Spectrometry: A new method for acylcarnitine profiling with potential for neonatal screening for inborn errors of metabolism. J Inher Metab Dis 1990;13:321-324.Johnson JV, Lee MS, Lee MR, Yost T. Triple Quadrupole MS/MS in Biomedical Research. Mass Spectrometry in Biomedical Research. 2 ed. New York: Plenum Press; 1986. Chapter 25:459-457.Millington DS, Chace DH. Carnitine and acylcarnitines in metabolic disease diagnosis and management. In: Desiderio DM, ed. Mass spectrometry: clinical and biomedical applications. Vol. I. New York: Plenum Press; 1992. p. 299-316.Libert R, Van Hoof F, Thillaye M, Vincent MF, Nassogne MC, Stroobant V, et al. Identification of new medium-chain acylcarnitines present in urine of a patient with medium-chain acyl-CoA dehydrogenase deficiency. J Inher Metab Dis1999;22:9-18.Shigmatsu Y, Hata I, Nakai A, Kikawa Y, Sudo M, Tanaka Y, et al. Prenatal Diagnosis of Organic Acidemias Based on Amniotic Fluid Levels of Acylcarnitines. Pediatr Res 1996;39:680-684.Rashed MS, Ozand PT, Bennet MJ, Barnard JJ, Govindaraza DR, Rinaldo P. Inborn errors of metabolism diagnosed in sudden death cases by acylcarnitine analysis of postmorten bile. Clin Chem 1995;41:1109-1114.Millington DS, Norwood DL, Kodo N, Roe CR, Inoue F. Application of fast atom bombardment with tandem mass spectrometry and liquid chromatography/mass spectrometry to the analysis of acylcarnitines in human urine, blood, and tissue. Anal Biochem 1989;180(2):331-9.Stevens RD, Hillman SL, Worthy S, Sanders D, Millington DS. Assay for free and total carnitine in human plasma using tandem mass spectrometry. Clin Chem 2000;46(5):727-9.Kodo N, Millington DS, Norwood DL, Roe CR. Quantitative assay of free and total carnitine using tandem mass spectrometry. Clin Chim Acta 1990;186(3):383-90.Lepage N, Aucoin S. Measurement of plasma/serum acylcarnitines using tandem mass spectrometry. Methods Mol Biol 2010;603:9-25.Osorio JH, Pourfarzam M. Hydrolysis of acylcarnitines during measurement in blood and plasma by tandem mass spectrometry. Acta Bioquim Clin Latinoam 2010;44(2):15-20.Budd MA, Tanaka K, Holmes LM, Efron ML, Crawford JD, Isselbacher KJ. Isovalefic acidemia: clinical features of a new genetic defect of leucine metabolism. N Engl J Med 1967;277:321-4.Osorio JH, Pourfarzam M. Early diagnosis of neurometabolic diseases by tandem mass spectrometry. Cord blood acylcarnitine profile. Rev Neurol 2004;38:11-16.Núm. 1 , Año 2010 : Enero - Juniohttps://revistasojs.ucaldas.edu.co/index.php/biosalud/article/download/5509/4975https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Osorio, José Henryoai:repositorio.ucaldas.edu.co:ucaldas/237372025-10-08T21:16:36Z

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