Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry.
Introducción: la determinación de los niveles de carnitina mediante el uso de espectrometría de masas en tándem, utilizando plasma humano, para la detección de alteraciones en la oxidación mitocondrial de ácidos grasos y acidurias orgánicas, ha sido descrita, sin embargo es deseable mejorar la preci...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad de Caldas
- Repositorio:
- Repositorio Institucional U. Caldas
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.ucaldas.edu.co:ucaldas/23693
- Acceso en línea:
- https://repositorio.ucaldas.edu.co/handle/ucaldas/23693
https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/4713
- Palabra clave:
- carnitina
espectrometría de masas en tándem
análisis
carnitine
tandem mass spectrometry
analysis
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
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Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry. Evaluación de cinco funciones de escaneado para el análisis de carnitina en plasma humano por espectrometria de masas en tándem.. |
| title |
Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry. |
| spellingShingle |
Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry. carnitina espectrometría de masas en tándem análisis carnitine tandem mass spectrometry analysis |
| title_short |
Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry. |
| title_full |
Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry. |
| title_fullStr |
Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry. |
| title_full_unstemmed |
Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry. |
| title_sort |
Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry. |
| dc.subject.none.fl_str_mv |
carnitina espectrometría de masas en tándem análisis carnitine tandem mass spectrometry analysis |
| topic |
carnitina espectrometría de masas en tándem análisis carnitine tandem mass spectrometry analysis |
| description |
Introducción: la determinación de los niveles de carnitina mediante el uso de espectrometría de masas en tándem, utilizando plasma humano, para la detección de alteraciones en la oxidación mitocondrial de ácidos grasos y acidurias orgánicas, ha sido descrita, sin embargo es deseable mejorar la precisión de los métodos usados para tal fin. Materiales y métodos: la carnitina en plasma fue analizada mediante espectrometría de masas en tándem mediante 5 diferentes métodos: derivatizando (4 métodos) y sin derivatizar (1 método).  Resultados: no se observó diferencia significativa entre las cinco funciones evaluadas. Conclusión: cualquiera de las funciones aquí evaluadas, puede ser utilizada para determinar carnitina en plasma. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-03-17T00:36:43Z 2022-03-17T00:36:43Z 2022-03-17 2025-10-08T21:16:16Z 2025-10-08T21:16:16Z |
| dc.type.none.fl_str_mv |
Artículo de revista http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/resource_type/c_2df8fbb1 Text info:eu-repo/semantics/article Journal article http://purl.org/redcol/resource_type/ART info:eu-repo/semantics/publishedVersion http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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publishedVersion |
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1657-9550 https://repositorio.ucaldas.edu.co/handle/ucaldas/23693 2462-960X https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/4713 |
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1657-9550 2462-960X |
| url |
https://repositorio.ucaldas.edu.co/handle/ucaldas/23693 https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/4713 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
58 2 52 11 Biosalud Osorio JH. Patología molecular de los errores hereditarios de la β-oxidación mitocondrial de los ácidos grasos: alcances en el diagnóstico y tratamiento. Biosalud 2006; 5:71-83. Osorio JH. Carnitine a very important osmolite for mitocondrial fatty acid oxidation. Biosalud 2006; 5:85-92. Millington DS, Kodo N, Norwood DL, Roe CR. Tandem Mass Spectrometry: A new method for acylcarnitine profiling with potential for neonatal screening for inborn errors of metabolism. J Inher Metab Dis 1990; 13:321-324. Millington DS, Chace DH. Carnitine and acylcarnitines in metabolic disease diagnosis and management. In: Desiderio DM, ed. Mass spectrometry: clinical and biomedical applications. Vol. I. New York: Plenum Press, New York; 1992. p. 299-316. Kodo N, Millington DS, Norwood DL, Roe CR. Quantative assay of free and total carnitine using tandem mass spectrometry. Clin Chim Acta 1989; 186:383-390. Sowell J, Fuqua M, Wood T. Quantification of Total and Free Carnitine in Human Plasma by Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry. J Chromatogr Sci 2011; 49(6):463-8. Johnson DW. An acid hydrolysis method for quantification of plasma free and total carnitine by flow injection tandem mass spectrometry. Clin Biochem 2010; 43(16-17):1362-7. Osorio JH, Pourfarzam M. Plasma free and total carnitine measured in children by tandem mass spectrometry. Braz J Med Biol Res 2002; 35:1265-1271. Hardy DT, Preece MA, Green A. Determination of plasma free carnitine by electrospray tandem mass spectrometry. Ann. Clin. Biochem 2001; 38: 665-670. Osorio JH, Loango N, Landazuri P. El tamizaje metabólico en los errores innatos del metabolismo. Biosalud 2008; 7:131-40. Osorio JH, Pourfarzam M. Implementación y estandarización de un método para la determinación de carnitina en plasma como herramienta diagnóstica de enfermedades hereditarias. Arch Med (Manizales) 2008; 8(2):126-133. Houten SM, Wanders RJ. A general introduction to the biochemistry of mitochondrial fatty acid β-oxidation. J Inherit Metab Dis 2010; 33(5):469-77. Osorio JH, Pourfarzam M. Early diagnosis of neurometabolic diseases by tandem mass spectrometry. Acylcarnitine profile from cord blood. Rev Neurol. 2004; 38(1):11-6. Thompson JW, Zhang H, Smith P, Hillman S, Moseley MA, Millington DS. Extraction and analysis of carnitine and acylcarnitines by electrospray ionization tandem mass spectrometry directly from dried blood and plasma spots using a novel autosampler. Rapid Commun Mass Spectrom. 2012; 26(21):2548-54. Lepage N, Aucoin S. Measurement of plasma/serum acylcarnitines using tandem mass spectrometry. Methods Mol Biol 2010; 603:9-25. Osorio JH. Uses of tandem mass spectrometry as a novel tool for research and diagnosis of intermediary metabolism diseases. Biosalud 2007; 6(1):161-74. Osorio JH, Pourfarzam M. Hydrolysis of acylcarnitines during measurement in blood and plasma by tandem mass spectrometry. Acta Bioquim Clin Latinoam 2010; 44(2):15-20. Osorio JH. Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry. Biosalud 2010; 9(1):9-16. Osorio JH. Effect of anticoagulant type during the blood sample collection process on the determination of the acylcarnitine profile in blood and plasma. Biosalud 2010; 9(2):9-13. Osorio JH, Pourfarzam M. Effect of adding the internal standard to blood samples, prior to the preparation of blood spots for acylcarnitine analysis. Biosalud 2010; 9(2):14-20. Núm. 2 , Año 2012 : Julio - Diciembre https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/download/4713/4302 |
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openAccess |
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application/pdf |
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Universidad de Caldas |
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Universidad de Caldas |
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https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/4713 |
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Universidad de Caldas |
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Evaluation of five different scan functions for the analysis of carnitine in human plasma by tandem mass spectrometry.Evaluación de cinco funciones de escaneado para el análisis de carnitina en plasma humano por espectrometria de masas en tándem..carnitinaespectrometría de masas en tándemanálisiscarnitinetandem mass spectrometryanalysisIntroducción: la determinación de los niveles de carnitina mediante el uso de espectrometría de masas en tándem, utilizando plasma humano, para la detección de alteraciones en la oxidación mitocondrial de ácidos grasos y acidurias orgánicas, ha sido descrita, sin embargo es deseable mejorar la precisión de los métodos usados para tal fin. Materiales y métodos: la carnitina en plasma fue analizada mediante espectrometría de masas en tándem mediante 5 diferentes métodos: derivatizando (4 métodos) y sin derivatizar (1 método).  Resultados: no se observó diferencia significativa entre las cinco funciones evaluadas. Conclusión: cualquiera de las funciones aquí evaluadas, puede ser utilizada para determinar carnitina en plasma.Abstract: the determination of carnitine levels through electrospray tandem mass spectrometry using human plasma for the detection of alteration in mitochondrial oxidation of fatty acids and organic acidemias, has been described; however improving precision in the method used for such end is desirable. Materials y methods: carnitine in plasma was measured by tandem mass spectrometry using five different methods: derivatized (4 methods) and underivatized (1 method). Results: no significant difference was observed between the five functions evaluated. Conclusion: any of the functions evaluated can be used to determine carnitine in plasma.Universidad de Caldas2022-03-17T00:36:43Z2025-10-08T21:16:16Z2022-03-17T00:36:43Z2025-10-08T21:16:16Z2022-03-17Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articleJournal articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85application/pdf1657-9550https://repositorio.ucaldas.edu.co/handle/ucaldas/236932462-960Xhttps://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/4713https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/4713eng5825211BiosaludOsorio JH. Patología molecular de los errores hereditarios de la β-oxidación mitocondrial de los ácidos grasos: alcances en el diagnóstico y tratamiento. Biosalud 2006; 5:71-83.Osorio JH. Carnitine a very important osmolite for mitocondrial fatty acid oxidation. Biosalud 2006; 5:85-92.Millington DS, Kodo N, Norwood DL, Roe CR. Tandem Mass Spectrometry: A new method for acylcarnitine profiling with potential for neonatal screening for inborn errors of metabolism. J Inher Metab Dis 1990; 13:321-324.Millington DS, Chace DH. Carnitine and acylcarnitines in metabolic disease diagnosis and management. In: Desiderio DM, ed. Mass spectrometry: clinical and biomedical applications. Vol. I. New York: Plenum Press, New York; 1992. p. 299-316.Kodo N, Millington DS, Norwood DL, Roe CR. Quantative assay of free and total carnitine using tandem mass spectrometry. Clin Chim Acta 1989; 186:383-390.Sowell J, Fuqua M, Wood T. Quantification of Total and Free Carnitine in Human Plasma by Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry. J Chromatogr Sci 2011; 49(6):463-8.Johnson DW. An acid hydrolysis method for quantification of plasma free and total carnitine by flow injection tandem mass spectrometry. Clin Biochem 2010; 43(16-17):1362-7.Osorio JH, Pourfarzam M. Plasma free and total carnitine measured in children by tandem mass spectrometry. Braz J Med Biol Res 2002; 35:1265-1271.Hardy DT, Preece MA, Green A. Determination of plasma free carnitine by electrospray tandem mass spectrometry. Ann. Clin. Biochem 2001; 38: 665-670.Osorio JH, Loango N, Landazuri P. El tamizaje metabólico en los errores innatos del metabolismo. Biosalud 2008; 7:131-40.Osorio JH, Pourfarzam M. Implementación y estandarización de un método para la determinación de carnitina en plasma como herramienta diagnóstica de enfermedades hereditarias. Arch Med (Manizales) 2008; 8(2):126-133.Houten SM, Wanders RJ. A general introduction to the biochemistry of mitochondrial fatty acid β-oxidation. J Inherit Metab Dis 2010; 33(5):469-77.Osorio JH, Pourfarzam M. Early diagnosis of neurometabolic diseases by tandem mass spectrometry. Acylcarnitine profile from cord blood. Rev Neurol. 2004; 38(1):11-6.Thompson JW, Zhang H, Smith P, Hillman S, Moseley MA, Millington DS. Extraction and analysis of carnitine and acylcarnitines by electrospray ionization tandem mass spectrometry directly from dried blood and plasma spots using a novel autosampler. Rapid Commun Mass Spectrom. 2012; 26(21):2548-54.Lepage N, Aucoin S. Measurement of plasma/serum acylcarnitines using tandem mass spectrometry. Methods Mol Biol 2010; 603:9-25.Osorio JH. Uses of tandem mass spectrometry as a novel tool for research and diagnosis of intermediary metabolism diseases. Biosalud 2007; 6(1):161-74.Osorio JH, Pourfarzam M. Hydrolysis of acylcarnitines during measurement in blood and plasma by tandem mass spectrometry. Acta Bioquim Clin Latinoam 2010; 44(2):15-20.Osorio JH. Effect of acetonitrile concentration on acylcarnitines measurement by tandem mass spectrometry. Biosalud 2010; 9(1):9-16.Osorio JH. Effect of anticoagulant type during the blood sample collection process on the determination of the acylcarnitine profile in blood and plasma. Biosalud 2010; 9(2):9-13.Osorio JH, Pourfarzam M. Effect of adding the internal standard to blood samples, prior to the preparation of blood spots for acylcarnitine analysis. Biosalud 2010; 9(2):14-20.Núm. 2 , Año 2012 : Julio - Diciembrehttps://revistasojs.ucaldas.edu.co/index.php/biosalud/article/download/4713/4302https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Osorio, José Henryoai:repositorio.ucaldas.edu.co:ucaldas/236932025-10-08T21:16:16Z |