Metagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficile

Clostridioides difficile may have a negative impact on gut microbiota composition in terms of diversity and abundance, thereby triggering functional changes supported by the differential presence of genes involved in significant metabolic pathways, such as short-chain fatty acids (SCFA). This work h...

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Autores:
Tipo de recurso:
Fecha de publicación:
2024
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/44825
Acceso en línea:
https://doi.org/10.1016/j.micres.2024.127739
https://repository.urosario.edu.co/handle/10336/44825
Palabra clave:
Clostridioides difficile
Metagenome-assembled genomes (MAGs)
Gut microbiota composition
Metabolic pathways
Dysbiosis
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License
Attribution-NonCommercial-NoDerivatives 4.0 International
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spelling 07f5f951-ff31-4b0f-94f0-7492d0a86f05a5210654-4eca-41a2-a75a-1fc1d2c552e2683b92cc-eb73-488f-a7aa-72e6f29a2e0048f75542-191b-4166-8449-e16e615fcab2d47463d6-6b94-4fef-9cf9-7e20e59e94231011716118600453006a5-2ec3-4faf-8e32-a9d7075d519d2025-01-26T18:32:37Z2025-01-26T18:32:37Z2024-08-012024-08-01Clostridioides difficile may have a negative impact on gut microbiota composition in terms of diversity and abundance, thereby triggering functional changes supported by the differential presence of genes involved in significant metabolic pathways, such as short-chain fatty acids (SCFA). This work has evaluated shotgun metagenomics data regarding 48 samples from four groups classified according to diarrhea acquisition site (community- and healthcare facility-onset) and positive or negative Clostridioides difficile infection (CDI) result. The metagenomic-assembled genomes (MAGs) obtained from each sample were taxonomically assigned for preliminary comparative analysis concerning differences in composition among groups. The predicted genes involved in metabolism, transport, and signaling remained constant in microbiota memberscharacteristic patterns were observed in MAGs and genes involved in SCFA butyrate and acetate metabolic pathways for each study group. A decrease in genera and species, as well as relative MAG abundance with the presence of the acetate metabolism-related gene, was evident in the HCFO/- group. Increased antibiotic resistance markers (ARM) were observed in MAGs along with the genes involved in acetate metabolism. The results highlight the need to explore the role of acetate in greater depth as a potential protector of the imbalances produced by CDI, as occurs in other inflammatory intestinal diseases.application/pdfhttps://doi.org/10.1016/j.micres.2024.127739https://repository.urosario.edu.co/handle/10336/44825engMicrobiological ResearchMicrobiological ResearchAttribution-NonCommercial-NoDerivatives 4.0 InternationalAbierto (Texto Completo)http://creativecommons.org/licenses/by-nc-sa/4.0/http://purl.org/coar/access_right/c_abf2Microbiological Researchinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURClostridioides difficileMetagenome-assembled genomes (MAGs)Gut microbiota compositionMetabolic pathwaysDysbiosisMetagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficilearticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Herrera, GiovannyCastañeda, SergioArboleda, Juan CamiloPérez-Jaramillo, Juan E.Patarroyo, Manuel AlfonsoRamírez González, Juan DavidMuñoz, MarinaORIGINALMetagenome-assembled_genomes_MAGs_suggest_an_acetate-driven_protective.pdfapplication/pdf3503300https://repository.urosario.edu.co/bitstreams/989c5442-7daf-478f-a507-5f915818b35e/download098b8817e5924fb5b3ce8a76c444d551MD51TEXTMetagenome-assembled_genomes_MAGs_suggest_an_acetate-driven_protective.pdf.txtMetagenome-assembled_genomes_MAGs_suggest_an_acetate-driven_protective.pdf.txtExtracted texttext/plain61483https://repository.urosario.edu.co/bitstreams/7508f3dd-e4de-4aa8-a8c2-a8acc0698a41/download0241702bd4cd8950c26d770f731d4fa6MD52THUMBNAILMetagenome-assembled_genomes_MAGs_suggest_an_acetate-driven_protective.pdf.jpgMetagenome-assembled_genomes_MAGs_suggest_an_acetate-driven_protective.pdf.jpgGenerated Thumbnailimage/jpeg4504https://repository.urosario.edu.co/bitstreams/620a5ad1-8496-443a-8924-76202c113297/download6caac2aa982971a9c7595bdb128e1f14MD5310336/44825oai:repository.urosario.edu.co:10336/448252025-03-05 10:16:27.548http://creativecommons.org/licenses/by-nc-sa/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttps://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Metagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficile
title Metagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficile
spellingShingle Metagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficile
Clostridioides difficile
Metagenome-assembled genomes (MAGs)
Gut microbiota composition
Metabolic pathways
Dysbiosis
title_short Metagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficile
title_full Metagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficile
title_fullStr Metagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficile
title_full_unstemmed Metagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficile
title_sort Metagenome-assembled genomes (MAGs) suggest an acetate-driven protective role in gut microbiota disrupted by Clostridioides difficile
dc.subject.spa.fl_str_mv Clostridioides difficile
Metagenome-assembled genomes (MAGs)
Gut microbiota composition
Metabolic pathways
Dysbiosis
topic Clostridioides difficile
Metagenome-assembled genomes (MAGs)
Gut microbiota composition
Metabolic pathways
Dysbiosis
description Clostridioides difficile may have a negative impact on gut microbiota composition in terms of diversity and abundance, thereby triggering functional changes supported by the differential presence of genes involved in significant metabolic pathways, such as short-chain fatty acids (SCFA). This work has evaluated shotgun metagenomics data regarding 48 samples from four groups classified according to diarrhea acquisition site (community- and healthcare facility-onset) and positive or negative Clostridioides difficile infection (CDI) result. The metagenomic-assembled genomes (MAGs) obtained from each sample were taxonomically assigned for preliminary comparative analysis concerning differences in composition among groups. The predicted genes involved in metabolism, transport, and signaling remained constant in microbiota members
publishDate 2024
dc.date.created.spa.fl_str_mv 2024-08-01
dc.date.issued.spa.fl_str_mv 2024-08-01
dc.date.accessioned.none.fl_str_mv 2025-01-26T18:32:37Z
dc.date.available.none.fl_str_mv 2025-01-26T18:32:37Z
dc.type.spa.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.spa.fl_str_mv https://doi.org/10.1016/j.micres.2024.127739
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/44825
url https://doi.org/10.1016/j.micres.2024.127739
https://repository.urosario.edu.co/handle/10336/44825
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartof.spa.fl_str_mv Microbiological Research
dc.rights.spa.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
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dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
Abierto (Texto Completo)
http://creativecommons.org/licenses/by-nc-sa/4.0/
http://purl.org/coar/access_right/c_abf2
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dc.publisher.spa.fl_str_mv Microbiological Research
dc.source.spa.fl_str_mv Microbiological Research
institution Universidad del Rosario
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dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
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