The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro

The coronavirus disease 2019 (COVID-19) has become a serious problem for public health since it was identified in the province of Wuhan (China) and spread around the world producing high mortality rates and economic losses. Nowadays, WHO recognizes traditional, complementary, and alternative medicin...

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Autores:
Zapata Builes, Wildeman
Rugeles López, María Teresa
Yepes, Lina M.
Zapata Cardona, María Isabel
Flórez Álvarez, Lizdany
Aguilar Jiménez, Wbeimar
Oliveros, Cristian
Yepes Perez, Andres
Herrera Calderon, Oscar
Tipo de recurso:
Article of investigation
Fecha de publicación:
2021
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
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OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/33487
Acceso en línea:
https://hdl.handle.net/20.500.12494/33487
Palabra clave:
SARS-CoV-2
COVID-19
Uncaria tomentosa
Cat´s claw
Antivirals
Severe acute respiratory syndrome coronavirus 2
SARS-CoV-2
COVID-19
Uncaria tomentosa
Cat´s claw
Antivirals
Severe acute respiratory syndrome coronavirus 2
Rights
openAccess
License
Atribución
id COOPER2_eb57345a36cbb8e74eb046b4fbf0fff1
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/33487
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
dc.title.spa.fl_str_mv The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro
title The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro
spellingShingle The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro
SARS-CoV-2
COVID-19
Uncaria tomentosa
Cat´s claw
Antivirals
Severe acute respiratory syndrome coronavirus 2
SARS-CoV-2
COVID-19
Uncaria tomentosa
Cat´s claw
Antivirals
Severe acute respiratory syndrome coronavirus 2
title_short The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro
title_full The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro
title_fullStr The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro
title_full_unstemmed The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro
title_sort The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro
dc.creator.fl_str_mv Zapata Builes, Wildeman
Rugeles López, María Teresa
Yepes, Lina M.
Zapata Cardona, María Isabel
Flórez Álvarez, Lizdany
Aguilar Jiménez, Wbeimar
Oliveros, Cristian
Yepes Perez, Andres
Herrera Calderon, Oscar
dc.contributor.author.none.fl_str_mv Zapata Builes, Wildeman
Rugeles López, María Teresa
Yepes, Lina M.
Zapata Cardona, María Isabel
Flórez Álvarez, Lizdany
Aguilar Jiménez, Wbeimar
Oliveros, Cristian
Yepes Perez, Andres
Herrera Calderon, Oscar
dc.subject.spa.fl_str_mv SARS-CoV-2
COVID-19
Uncaria tomentosa
Cat´s claw
Antivirals
Severe acute respiratory syndrome coronavirus 2
topic SARS-CoV-2
COVID-19
Uncaria tomentosa
Cat´s claw
Antivirals
Severe acute respiratory syndrome coronavirus 2
SARS-CoV-2
COVID-19
Uncaria tomentosa
Cat´s claw
Antivirals
Severe acute respiratory syndrome coronavirus 2
dc.subject.other.spa.fl_str_mv SARS-CoV-2
COVID-19
Uncaria tomentosa
Cat´s claw
Antivirals
Severe acute respiratory syndrome coronavirus 2
description The coronavirus disease 2019 (COVID-19) has become a serious problem for public health since it was identified in the province of Wuhan (China) and spread around the world producing high mortality rates and economic losses. Nowadays, WHO recognizes traditional, complementary, and alternative medicine for treating COVID-19 symptoms. Therefore, we investigated the antiviral potential of the hydroalcoholic extract of Uncaria tomentosa stem bark from Peru against SARS-CoV-2 in vitro. The antiviral activity of U. tomentosa against SARS-CoV-2 in vitro was assessed in Vero E6 cells using cytopathic effect (CPE) and plaque reduction assay. After 48h of treatment, U. tomentosa showed an inhibition of 92.7 % of SARS-CoV-2 at 25.0 µg/mL (p<0.0001) by plaque reduction assay on Vero E6 cells. In addition, U. tomentosa, induced a reduction of 98.6 % (p=0.02) and 92.7 % (p=0.03) in the CPE caused by SARS-CoV-2 on Vero E6 cells at 25 µg/mL and 12.5 µg/mL, respectively. The EC50 calculated for U. tomentosa extract by plaque reduction assay was 6.6 µg/mL (4.89 – 8.85 µg/mL) for a selectivity index of 4.1. The EC50 calculated for U. tomentosa extract by TCID50 assay was 2.57 µg/mL (1.05 – 3.75 µg/mL) for a selectivity index of 10.54. These results showed that U. tomentosa known as Cat´s claw has antiviral effect against SARS-CoV-2 observed as a reduction in the viral titer and CPE after 48h of treatment on Vero E6 cells. Therefore, we hypothesized that U. tomentosa stem bark, could be promissory to the development of new therapeutic strategies against SARS-CoV-2.
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2021-03-01T13:05:22Z
dc.date.available.none.fl_str_mv 2021-03-01T13:05:22Z
dc.date.issued.none.fl_str_mv 2021-02-24
dc.type.none.fl_str_mv Artículos Científicos
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dc.identifier.issn.spa.fl_str_mv 1741-4288
dc.identifier.uri.spa.fl_str_mv 10.1155/2021/6679761
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/33487
dc.identifier.bibliographicCitation.spa.fl_str_mv Yepes Pérez, A. F., Herrera Calderón, O., Oliveros, C. A., Flórez Álvarez, L., Zapata Cardona, M. I., Yepes, L., Aguilar Jiménez, W., Rugeles, M. T. and Zapata, W. (2021). The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro. Evidence-Based Complementary and Alternative Medicine, vol. 2021, Article ID 6679761, 1-11. https://doi.org/10.1155/2021/6679761.
identifier_str_mv 1741-4288
10.1155/2021/6679761
Yepes Pérez, A. F., Herrera Calderón, O., Oliveros, C. A., Flórez Álvarez, L., Zapata Cardona, M. I., Yepes, L., Aguilar Jiménez, W., Rugeles, M. T. and Zapata, W. (2021). The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro. Evidence-Based Complementary and Alternative Medicine, vol. 2021, Article ID 6679761, 1-11. https://doi.org/10.1155/2021/6679761.
url https://hdl.handle.net/20.500.12494/33487
dc.relation.isversionof.spa.fl_str_mv https://www.hindawi.com/journals/ecam/2021/6679761/
dc.relation.ispartofjournal.spa.fl_str_mv Evidence-Based Complementary and Alternative Medicine
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Zheng Feei Ma
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spelling Zapata Builes, WildemanRugeles López, María TeresaYepes, Lina M.Zapata Cardona, María IsabelFlórez Álvarez, LizdanyAguilar Jiménez, WbeimarOliveros, CristianYepes Perez, AndresHerrera Calderon, Oscar20212021-03-01T13:05:22Z2021-03-01T13:05:22Z2021-02-241741-428810.1155/2021/6679761https://hdl.handle.net/20.500.12494/33487Yepes Pérez, A. F., Herrera Calderón, O., Oliveros, C. A., Flórez Álvarez, L., Zapata Cardona, M. I., Yepes, L., Aguilar Jiménez, W., Rugeles, M. T. and Zapata, W. (2021). The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In Vitro. Evidence-Based Complementary and Alternative Medicine, vol. 2021, Article ID 6679761, 1-11. https://doi.org/10.1155/2021/6679761.The coronavirus disease 2019 (COVID-19) has become a serious problem for public health since it was identified in the province of Wuhan (China) and spread around the world producing high mortality rates and economic losses. Nowadays, WHO recognizes traditional, complementary, and alternative medicine for treating COVID-19 symptoms. Therefore, we investigated the antiviral potential of the hydroalcoholic extract of Uncaria tomentosa stem bark from Peru against SARS-CoV-2 in vitro. The antiviral activity of U. tomentosa against SARS-CoV-2 in vitro was assessed in Vero E6 cells using cytopathic effect (CPE) and plaque reduction assay. After 48h of treatment, U. tomentosa showed an inhibition of 92.7 % of SARS-CoV-2 at 25.0 µg/mL (p<0.0001) by plaque reduction assay on Vero E6 cells. In addition, U. tomentosa, induced a reduction of 98.6 % (p=0.02) and 92.7 % (p=0.03) in the CPE caused by SARS-CoV-2 on Vero E6 cells at 25 µg/mL and 12.5 µg/mL, respectively. The EC50 calculated for U. tomentosa extract by plaque reduction assay was 6.6 µg/mL (4.89 – 8.85 µg/mL) for a selectivity index of 4.1. The EC50 calculated for U. tomentosa extract by TCID50 assay was 2.57 µg/mL (1.05 – 3.75 µg/mL) for a selectivity index of 10.54. These results showed that U. tomentosa known as Cat´s claw has antiviral effect against SARS-CoV-2 observed as a reduction in the viral titer and CPE after 48h of treatment on Vero E6 cells. Therefore, we hypothesized that U. tomentosa stem bark, could be promissory to the development of new therapeutic strategies against SARS-CoV-2.The coronavirus disease 2019 (COVID-19) has become a serious problem for public health since it was identified in the province of Wuhan (China) and spread around the world producing high mortality rates and economic losses. Nowadays, WHO recognizes traditional, complementary, and alternative medicine for treating COVID-19 symptoms. Therefore, we investigated the antiviral potential of the hydroalcoholic extract of Uncaria tomentosa stem bark from Peru against SARS-CoV-2 in vitro. The antiviral activity of U. tomentosa against SARS-CoV-2 in vitro was assessed in Vero E6 cells using cytopathic effect (CPE) and plaque reduction assay. After 48h of treatment, U. tomentosa showed an inhibition of 92.7 % of SARS-CoV-2 at 25.0 µg/mL (p<0.0001) by plaque reduction assay on Vero E6 cells. In addition, U. tomentosa, induced a reduction of 98.6 % (p=0.02) and 92.7 % (p=0.03) in the CPE caused by SARS-CoV-2 on Vero E6 cells at 25 µg/mL and 12.5 µg/mL, respectively. The EC50 calculated for U. tomentosa extract by plaque reduction assay was 6.6 µg/mL (4.89 – 8.85 µg/mL) for a selectivity index of 4.1. The EC50 calculated for U. tomentosa extract by TCID50 assay was 2.57 µg/mL (1.05 – 3.75 µg/mL) for a selectivity index of 10.54. These results showed that U. tomentosa known as Cat´s claw has antiviral effect against SARS-CoV-2 observed as a reduction in the viral titer and CPE after 48h of treatment on Vero E6 cells. 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DC. - ethnomedicinal use and new pharmacological, toxicological and botanical results,” Journal of Ethnopharmacology, vol. 64, no. 1, pp. 23–34, 1998.SARS-CoV-2COVID-19Uncaria tomentosaCat´s clawAntiviralsSevere acute respiratory syndrome coronavirus 2SARS-CoV-2COVID-19Uncaria tomentosaCat´s clawAntiviralsSevere acute respiratory syndrome coronavirus 2The Hydroalcoholic Extract of Uncaria tomentosa (Cat’s Claw) Inhibits the Infection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) In VitroArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-84334https://repository.ucc.edu.co/bitstreams/cd5ea5bf-f1ec-4957-88db-5d044e60259a/download3bce4f7ab09dfc588f126e1e36e98a45MD52ORIGINALuncaria tomentosa UCC 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