Species-specific PCR for neotropical felids

Felids are crucial components of their ecosystems, often serving as top predators and exerting profound effects on their environment. Some felids require extensive forest habitats to thrive and can be considered an "umbrella species," whose conservation benefits other species in the same a...

Full description

Autores:
Arce Orrego, Valeria
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad CES
Repositorio:
Repositorio Digital - Universidad CES
Idioma:
eng
OAI Identifier:
oai:repository.ces.edu.co:10946/7544
Acceso en línea:
https://hdl.handle.net/10946/7544
Palabra clave:
Felidae
Umbrella species
Simplex PCR
Genetic identification
Rights
closedAccess
License
http://purl.org/coar/access_right/c_14cb
Description
Summary:Felids are crucial components of their ecosystems, often serving as top predators and exerting profound effects on their environment. Some felids require extensive forest habitats to thrive and can be considered an "umbrella species," whose conservation benefits other species in the same area. Therefore, accurate genetic identification of these animals through minimally invasive and highly accurate techniques is of foremost importance, given their elusive nature and the challenges of monitoring them in the wild. Accordingly, six simplex PCRs have been standardized to identify several felid species, including Leopardus pardalis, Leopardus wiedii, Leopardus tigrinus, Puma concolor, Puma yagouaroundi and Panthera onca, using species-specific primer sets and several types of samples such as blood, muscle, hair, feces, among others. These tests have shown to be a reliable and cost-effective method for their identification and can have multiple applications regarding their conservation and future research.